Serulein ile Oluşturulmuş Akut Pankreatit Modelinde Astaksantin ve Koenzim Q10’nin Etkisi Sinerjistik Değildir

Öz

 

Amaç: Astaksantin (ATX) ve Koenzim Q10'un (Ubiquinone) çeşitli antiinflamatuvar etkileri farklı inflamasyon modellerinde incelenmiştir. Bu çalışma, sıçan akut pankreatit modelinde astaksantin ve Koenzim Q10'un sinerjistik etkisini araştırmayı amaçlamaktadır.

Gereç ve Yöntem: 24 adet Wistar sıçan; kontrol (C), taşıyıcı  kontrol (AP), astaksantin (ATX; 40 mg / kg), astaksantin + koenzim Q10 (ATX + Q10; ATX+Q10; 40 mg/kg and 1 gr/kg) olarak gruplandırıldı.  Bir saat ara ile iki kez serulein (50 ug / kg) uygulandı. İlk serulein enjeksiyonundan 7 saat sonra sıçanlar öldürüldü. Pankreatik oksidatif hasar, lipaz ve amilazın serum düzeyinde, miyeloperoksidaz aktivitesi (MPO), malondialdehit (MDA), luminol ve lucigenin doku seviyelerinde artışı ve glutatyonın doku seviyesindeki azalması ile değerlendirildi.

Bulgular: Tüm AP gruplarında MDA, MPO artarken GSH azaldı (p <0.001). ATX veya ATX + Q10, artmış MDA ve MPO düzeylerini anlamlı şekilde düşürürken (p <0.001) azalmış GSH düzeyinde artışa neden oldu. (p <0.01) . Her iki tedavi de luminol düzeylerini arttırdı (p <0.001) (p <0.01). Lucigenin Q10 grubunda belirgin olarak azaldı (p <0.01).

Sonuç: Antioksidan kombinasyon tedavisi pankreatik dokudaki oksidatif hasarı tek başına astaksantinden daha iyi hafifletmemektedir.

 

Anahtar Kelimeler

• Astaksantin,Koenzim Q10,oksidatif hasar

Astaxanthin and Coenzyme Q10 are not synergistic against oxidative damage in cerulein-induced acute pancreatitis

Abstract

Background/Aim: The anti-inflammatory effects of Astaxanthin (ATX) and Coenzyme Q10 (Ubiquinone) are studied in different inflammation models. The study aims to investigate the synergistic effect of astaxanthin and Coenzyme Q10 in a rat model of acute pancreatitis. Methods: Twenty-four female Wistar rats were grouped as control (C), carrier-treated control (AP), astaxanthin-treated (ATX; 40 mg/kg), astaxanthin+coenzyme Q10- treated (ATX+Q10; 40 mg/kg and 1 gr/kg) groups. Cerulein was administered (50 µg/kg) twice, one hour apart. Seven hours after the first cerulein injection, the rats were sacrificed. Pancreatic oxidative damage was evaluated with an increase in the serum activity of lipase and amylase, tissue levels of myeloperoxidase activity (MPO), malondialdehyde (MDA), luminol and lucigenin and decrease of glutathione. Results: In all AP groups, MDA and MPO increased while GSH decreased (P<0.001). ATX and ATX+Q10 both decreased MDA and MPO (P<0.001) and increased GSH (P<0.01). Both therapies significantly increased luminol levels (P<0.001, and P<0.01, respectively). Lucigenin markedly decreased in the ATX+Q10 group (P<0.01). Conclusion: Antioxidant combination therapy does not alleviate oxidative damage in pancreatic tissue better than astaxanthin alone.

Keywords

• Astaxanthin
• Coenzyme Q10
• Oxidative Damage
• Acute pancreatitis

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