An experimental study on the long-term and short-term effects of PRP treatment on the endometrium and ovaries

Abstract

Aim: It has been reported that ovaries have stem cells, and they can be used in the treatment of patients with low ovarian reserves by maturing them through some growth factors. PRP, a new therapy, has many bioactive compounds including growth factors. This study aimed to investigate the long and short-term biochemical and histopathological effects of platelet-rich plasma (PRP) treatments on the lining of the uterus and the ovaries in rats. Methods: Female rats (n = 21) were randomly divided into 3 groups: A sham group, a short-term PRP group and a long-term PRP group. At the end of the study, anti-Mullerian hormone (AMH) and follicle-stimulating hormone (FSH) levels were examined in blood samples obtained from the intracardiac area, and the ovaries and uterus of the rats were examined to determine the histopathological and immunohistochemical findings. Results: The results of this study demonstrated that, in the blood samples of the rats, the AMH levels increased and FSH levels decreased in the short-term PRP group (P<0.05). When the uteri and ovaries of the rats were examined histopathologically, positive and beneficial effects of the PRP therapy were found in the short-term PRP group. However, considering the long-term results, the positive effects of PRP decrease as time goes on. Conclusion: This study showed that PRP application delivers beneficial effects to the uterus and ovaries in female rats in the short term.

Keywords

• Anti Mullerian Hormone
• follicle stimulating hormone
• ovary
• platalet-rich plasma
• rat
• endometrium

PRP tedavisinin endometriyum ve yumurtalıklar üzerindeki uzun vadeli ve kısa vadeli etkileri üzerine deneysel bir çalışma

Abstract

Amaç: Yumurtalıkların kök hücrelere sahip olduğu ve yumurtalık rezervi düşük olan hastaların bazı büyüme faktörleri ile olgunlaştırılarak tedavisinde kullanılabileceği bildirilmiştir. Yeni bir tedavi olan PRP, büyüme faktörleri dahil olmak üzere birçok biyoaktif bileşiğe sahiptir. Bu çalışma, sıçanlarda trombositten zengin plazma (PRP) tedavilerinin uterus ve yumurtalıkların iç yüzeyine uzun ve kısa vadeli biyokimyasal ve histopatolojik etkilerini araştırmayı amaçladı. Yöntemler: Dişi sıçanlar (n=21) rastgele 3 gruba ayrıldı: Sham grup, kısa süreli bir PRP grubu ve bir uzun süreli PRP grubu. Çalışma sonunda intrakardiyak alandan alınan kan örneklerinde anti-Mullerian hormonu (AMH) ve folikül stimüle edici hormon (FSH) seviyeleri incelendi ve ratların yumurtalık ve uterusu incelendiğinde histopatolojik ve immünohistokimyasal bulgular. Bulgular: Sıçanların kan örneklerinde kısa süreli PRP grubunda AMH düzeylerinin arttığını ve FSH düzeylerinin düştüğünü göstermiştir (P<0.05). Sıçanların rahim ve yumurtalıkları histopatolojik olarak incelendiğinde, kısa süreli PRP grubunda PRP tedavisinin olumlu ve faydalı etkileri tespit edildi. Ancak uzun vadeli sonuçlar dikkate alındığında PRP'nin olumlu etkileri zaman geçtikçe azalmaktadır. Sonuç: PRP uygulamasının kısa vadede dişi sıçanlarda rahim ve yumurtalıklara faydalı etkiler sağladığını göstermiştir.

Keywords

• Anti-Mullerian hormone
• Follicle-stimulating hormone
• Over
• Platelet-Rich plasma
• Rat
• Endometriyum

Supporting Institution

MADDİ DESTEĞİMİZ YO

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