Reelin levels in inflammatory bowel disease: A case-control study

Abstract

Background/Aim: Crohn's Disease (CD) and Ulcerative Colitis (UC) are grouped as Inflammatory Bowel Diseases (IBD). There are many similarities between these two diseases, and CD and UC cases cannot be separated at a rate varying between 5% and 10%. Reelin is an extracellular matrix protein first known for its vital role in neuronal migration. Studies in rodent small intestine suggested that reelin protects the organism from intestinal pathologies. In a 5-year retrospective case-control study, we aimed to detect the effectiveness of serum reelin level in patients with IBD in determining the severity and activation of the disease and compare healthy volunteers with patients in terms of inactivation and remission. Methods: The data of all 194 IBD patients diagnosed at Beyazit Training and Research Hospital between 2011-2015 were retrospectively reviewed. The patients were matched with 30 healthy volunteers. Risk factors were assessed by multivariate logistic regression analysis. Results: The serum reelin levels were similar between UC and CD patients, the control group, UC and CD groups (P=0.067), and those with active disease or disease in remission, and did not differ according to disease behavior or location of involvement. Conclusions: Our study shows that Reelin cannot be used as an activation/remission marker in IBD. In addition, it does not differentiate between UC and CD.

Keywords

• Reelin
• Inflammatory bowel diseases
• Crohn
• Ulcerative colitis

Öz

ÖZET İnflamatuvar barsak hastalıkları aktivasyon ve remisyon ile giden ve etiyolojisi bilinmeyen kronik intestinal hastalıklardır. Aktiviteyi belirlemek inflamatuvar barsak hastalığında tedavinin düzenlenmesi ve prognozu göstermesi açısından büyük önem taşır. Aktiviteyi belirlemek için halen ortak bir konsensüs oluşmamıştır. Reelin de nörolojik gelişim ve sinaptik plastisite önemli roller oynamaktadır. Reelinin şizofreni patogenezinde rolü bulunmaktadır. Araştırma Ankara Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi Gastroenteroloji – İBH Polikliniği’nde gerçekleştirilmiştir. Takip ve tedavisi yapılan İBH vakası 194 hasta ve 30 sağlıklı kontrol dâhil edilmiştir. Hastaların verileri poliklinik dosya ve hastane bilgi işlem sisteminden elde edilmiştir. CH aktivite indeksi ile UC True Love aktivite indeksi kullanılmış ve serumda Reelin düzeyi çalışılmıştır. Çalışma retrospektif olup hastalara ek herhangi bir işlem uygulanmamıştır. Araştırmada da; Reelin düzeyleri ÜK total: 0,6216 +/- 1,0835 aktif:0,3846 +/- 0,4850 remisyon:0,4354 +/- 0,4354 idi. CD’da Reelin total:0,3993 +/-0,5101 aktif: 0,3675 +/- 0,5789 remisyon: 0,4354 +/- 0,5789 idi ve kontrol grubunda: 0,5576 +/- 0,6142 olarak saptandı. ÜK ve CH arasında serum Reelin düzeyi ile anlamlı fark saptanmamış, ÜK ve CH ile ayrı ayrı kontrol grubu ile olan karşılaştırma sonucu anlamlı fark bulunmamıştır. (p > 0,05). Bu bağlamda da görülmektedir ki, serum Reelin düzeyinin ÜK ve CH vakalarını etkileme düzeyi farklılık içermemektedir. Araştırma kapsamında, ÜK ve CH aktif ve kontrol grubu arasında istatistik bakımdan anlamlı bulunmamıştır (p > 0,05). ÜK reelin düzeyleri:0,6216 +/- 1,0835 (X2 = 0,109; p > 0,05) ve aktif ÜK reelin düzeyleri: 0,3846 +/- 0,4850 (X2 = 0,820; p > 0,05) tutulum görülen lokasyona göre anlamlı farklılık göstermemektedir. Gruplar bazında genel olarak değerlendirildiğinde de görülmektedir ki, Reelin düzeyleri tutulum görülen lokasyona göre anlamlı farklılık göstermemektedir (X2 = 1,007; p> 0,05). Sonuç olarak çalışmamız; Reelinin, İBH’da aktivasyon remisyon markerı olarak kullanılamayacağını ve ayrıca ÜK-Crohn arasında ayrıcı tanıda belirteç olmadığını göstermektedir

Anahtar Kelimeler

• Reelin, İnflamatuvar Barsak Hastalığı, Crohn Hastalığı, Ülseratif Kolit.

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