Gastroprotective effects of hydrogen sulfide, carbon monoxide and nitric oxide on an experimental ulcer model in rats

Abstract

Background/Aim: Gastric mucosal injury induced by several agents such as ethanol, stress or nonsteroidal anti-inflammatory drugs (NSAIDs) is a common severe disorder. Hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) are gaseous autacoids that are endogenously produced in mammalian tissues. Recently, several studies confirmed that H2S, CO and NO play a role in gastroprotection. Our work aimed to evaluate and compared the gastroprotective effects of H2S, CO and NO on ethanol-, indomethacin- and stress-induced rat ulcer models. Methods: The effects of NaHS (5 mg/kg), CORM-2 (5 mg/kg) and L-arginine (100 mg/kg) were investigated on gastric ulcer models induced by ethanol (1 ml 96% i.g.), stress (cold+immobility) and indomethacin (40 mg/kg i.g.). The ulcer index, gastric mucus secretion, free and total acidity, and levels of TNF-α, PGE2, MDA GSH, COX-1, COX-2 were measured. Results: NaHS and CORM-2 decreased the increased TNF-α and MDA levels in ethanol-induced ulcer. L-arginine reduced mucin secretion, TNF-α and GSH levels in stress-induced gastric ulcer. Conclusion: The present study showed that H2S and CO may have gastroprotective activity against ethanol-induced ulcers and NO may be gastroprotective against stress-induced ulcers.

Keywords

• Gasotransmitters
• Hydrogen sulfide
• Carbon monoxide
• Nitric oxide
• Ulcer

Hidrojen sülfür, karbon monoksit ve nitrik oksidin sıçanlarda deneysel ülser modelleri üzerindeki gastroprotektif etkileri

Öz

Giriş / Amaç: Mide mukozal hasarı, etanol, stres veya nonsteroid antiinflamatuvar ilaçlar (NSAID'ler) gibi çeşitli ajanların neden olduğu yaygın görülen ciddi bir hastalıktır. Hidrojen sülfür (H2S), karbon monoksit (CO) ve nitrik oksit (NO), memeli dokularında endojen olarak üretilen gaz halindeki otakoidlerdir. Son zamanlarda birkaç çalışma, H2S, CO ve NO'nun gastroproteksiyonda rol oynadığını doğrulamıştır. Çalışmamız, H2S, CO ve NO'nun etanol, indometazin ve stres kaynaklı sıçan ülseri modelleri üzerindeki gastroprotektif etkilerini değerlendirmeyi ve karşılaştırmayı amaçlamıştır. Yöntemler: NaHS (5 mg/kg), CORM-2 (5 mg/kg) ve L-argininin (100 mg/kg) etkileri, etanol (1 ml% 96 ​​i.g), stres (soğuk + hareketsizlik) ve indometasin (40 mg/kg, i.g) ile indüklenen mide ülseri modellerinde araştırılmıştır. . Ülser indeksi, mide mukus sekresyonu, serbest ve toplam asidite, TNF-α, PGE2, MDA GSH, COX-1, COX-2 seviyeleri ölçülmüştür. Bulgular: H2S ve CO'nun etanol ile indüklenen ülsere karşı ve NO'nun stres kaynaklı ülsere karşı koruyucu etkileri olduğu tespit edilmiştir. Sonuç: Bu çalışma, H2S ve CO'nun etanol kaynaklı ülserlere ve NO'nun strese bağlı ülserlere karşı koruyucu aktiviteye sahip olabileceğini göstermiştir.

Anahtar Kelimeler

• gaz transmitterler
• hidrojen sülfür
• nitrik oksit
• karbon monoksit

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