Relationship between vitamin D receptor gene polymorphisms and vitamin D levels in children

Abstract

Background/Aim: Vitamin D exerts its effects in the body through its receptors. Polymorphisms in vitamin D Receptor (VDR) gene are known to cause certain diseases and affect vitamin D levels. In this study, we planned to examine the relationship between vitamin D levels and Vitamin D gene polymorphisms among children. Methods: The study group included 124 healthy children living in the same region. Vitamin D (VitD), Parathyroid Hormone (PTH), Alkaline phosphatase (ALP), Calcium (Ca), Phosphorus (P) and Magnesium (Mg) were examined in the blood samples taken. In terms of measured Vitamin D levels, children were divided into group 1 (Vitamin D < 20 ng/mL) and group 2 (Vitamin D ≥ 20 ng/mL). Deoxyribonucleic Acid (DNA) was isolated from the serum sample, VDR ApaI and TaqI polymorphisms were determined by Polymerase Chain Reaction (PCR) method, and comparisons were made between groups. Results: The overall mean age of the children included in the study was 8.11 (4.98) years. The mean ages of participants in Groups 1 and 2 were 9.38 (4.87) years and 6.38 (4.62) years, respectively (P=0.091). The mean vitamin D levels of Groups 1, 2 and overall were 13.82 (3.29) ng/mL, 33.96 (20.47) ng/mL, and 23.49 (21.54) ng/mL, respectively (P=0.509). The two groups were similar in terms of serum ALP, PTH, Ca, Mg and P levels (P>0.05 for all). VDR polymorphisms were found to have no effect on Vitamin D levels. Conclusion: In our study, no relationship was found between the genotypes of ApaI and TaqI polymorphisms and Vitamin D levels. This study is important in terms of remarking the fact that Vitamin D deficiency is still a public health problem and its contributions to VDR gene polymorphism research.

Keywords

• Vitamin D
• Vitamin D Receptor
• Polymorphism

References

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