Research Article
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Taxifolinin biyokimyasal ve histopatolojik değerlendirilmesi: Rat modelinde karaciğer iskemi reperfüzyon hasarı üzerine deneysel çalışma

Year 2019, Volume: 3 Issue: 7, 494 - 497, 29.07.2019
https://doi.org/10.28982/josam.587598

Abstract

Amaç: Karaciğer rezeksiyonu ve transplantasyon
sırasında uygulanan iskemi reperfüzyon (I/R) prosedürü karaciğerde oksidatif
hasara yol açmaktadır. Bu çalışmanın amacı ratlarda iskemi reperfüzyonun neden olduğu
karaciğer hasarına taxifolinin etkisini incelemektir. Yöntemler: Albino Wistar
erkek ratlar her grupta 6 rat olmak üzere üç gruba ayrıldı: karaciğer iskemi
reperfüzyon (LIR), 50 mg/kg taxifolin + karaciğer iskemi reperfüzyon hasarı
(TLIR) ve sham operasyon (SHAM). Thiopental sodium anestezisinden bir saat önce
TLIR grubuna 50 mg/kg taxifolin; LIR ve SHAM grubuna ise distile su uygulandı.
TLIR ve LIR gruplarında hepatik arter, portal ven ve safra kanalına bir saat
iskemi ve altı saat reperfüzyon oluşturmak üzere klemp yerleştirildi. SHAM
grubunda herhangi bir prosedür uygulanmadan karın boşluğu cerrahi dikişle
kapatıldı. Bu periyodun sonunda ratlar yüksek doz anestezi ile sakrifiye
edildi. Karaciğer dokuları biyokimyasal ve histopatolojik inceleme için
çıkarıldı. I/R prosedüründe SHAM grubu ile kıyaslandığında karaciğer dokusunda
MDA (P<0,001), AST, ALT (P<0,001) değerlerinde anlamlı artış
ve tGSH (P<0,001) değerinde azalma
görüldü. Taxifolin uygulanan grupta bu etki baskılandı.

Bulgular: I/R grubunda patolojik olarak dilate
konjeste kan damarları, hemoraji, karaciğer parankiminde destrüksiyon ve ödem
gözlendi. Taxifolin uygulanan grupta karaciğer dokusu orta derecede sinusoidal
konjesyon dışında normale yakın görünümdeydi.





Sonuç: Sonuçlarımız taxifolinin iskemi reperfüzyon
sonrası oluşan karaciğer hasarını önlemede etkili olduğunu göstermektedir.

References

  • 1. Jeon BR, Yeom DH, Lee SM. Protective effect of allopurinol on hepatic energy metabolism in ischemic and reperfused rat liver. Shock. 2001;15:112-7.
  • 2. Markmann JF. Relaxing liver ischemia reperfusion injury down 1 notch. Am J Transplant. 2018;18:1587-8.
  • 3. Kong R, Gao Y, Sun B. The strategy of combined ischemia preconditioning and salvianolic acid-B pretreatment to prevent hepatic ischemia-reperfusion injury in rats. Dig Dis Sci. 2009;54:2568-76.
  • 4. Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am. 1992;72:65-83.
  • 5. Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology 2001;94:1133-8.
  • 6. Zhai Y, Busuttil RW, Kupiec‐Weglinski JW. Liver Ischemia and Reperfusion Injury: New Insights into Mechanisms of Innate—Adaptive Immune‐Mediated Tissue Inflammation. Am J Transplant 2011;11:1563-9.
  • 7. Jaeschke H. Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning. Am J Physiol Gastrointest Liver Physiol 2003;284:15-26.
  • 8. Kisaoglu A, Ozogul B, Turan MB Damage induced by paracetamol compared with N-acetylcysteine. J Chin Med Assoc. 2014;77:463-8.
  • 9. Deng WS, Xu Q, Liu Y, Jiang CH, Zhou H, Gu L. Effects of melatonin on liver function and lipid peroxidation in a rat model of hepatic ischemia/reperfusion injury. Exp Ther Med. 2016;11:1955-60.
  • 10. Wang Q, Wang L, Gaiping L, Baoxian Y. A simple and sensitive method for determination of taxifolin on palladium nanoparticles supported poly (diallyldimethylammonium chloride) functionalized graphene modified electrode. Talanta 2017;164:323-9.
  • 11. Topal F, Nar M, Gocer H Antioxidant activity of taxifolin: An activity–structure relationship. J Enzyme Inhib Med Chem. 2016;31:674-83.
  • 12. Gupta MB, Bhalla TN, Gupta GP, Mitra CR, Bhargava KP. Anti-inflammatory activity of taxifolin. Jpn J Pharmacol. 1971;21:377-82.
  • 13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351-8.
  • 14. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;25:192-205.
  • 15. Halliwell B, Chirico S. Lipid peroxidation: its mechanism, measurement, and significance. Am J Clinl Nutr. 1993;5:715-25.
  • 16. Goulart M, Batoréu MC, Rodrigues AS, Laires A, Rueff J. Lipoperoxidation products and thiol antioxidants in chromium exposed workers. Mutagenesis 2005;20:311-5.
  • 17. Sener MT, Suleyman H, Hacimuftuoglu A. Estimating the postmortem interval by the difference between oxidant/antioxidant parameters in liver tissue. Adv Clin Exp Med. 2012;21:727-33.
  • 18. Demiryilmaz I, Sener E, Cetin N Biochemically and histopathologically comparative review of thiamine’s and thiamine pyrophosphate’s oxidative stress effects generated with methotrexate in rat liver. Med Sci Monit. 2012;18:475-81.
  • 19. Coskun AK, Yigiter M, Oral A The effects of Montelukast on antioxidant enzymes and proinflammatory cytokines on the heart, liver, lungs, and kidneys in a rat model of cecal ligation and puncture–induced sepsis. Sci World J. 2011;11:1341-56.
  • 20. Ingec M, Isaoglu U, Yilmaz M. Prevention of ischemia-reperfusion injury in rat ovarian tissue with the on-off method. J Physiol Pharmacol 2011;62:575-82.
  • 21. Altuner D, Cetin N, Suleyman B Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney. Indian J Pharmacol. 2013;45:339-43.
  • 22. Polat B, Suleyman H, Alp HH. Adaptation of rat gastric tissue against indomethacin toxicity. Chem Biol Interact. 2010;186:82-9.
  • 23. Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 2003;189:41-54.
  • 24. Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med. 2013;45:47-9.
  • 25. Murray RK, Granner DK, Mayes PA, Rodwell VM. Harper's Biochemistry. 25th ed, McGraw-Hill Press: USA; 2000.
  • 26. Yilmaz I, Demiryilmaz I, Turan MI, Cetin N, Gul MA, Suleyman H. The effects of thiamine and thiamine pyrophosphate on alcohol-induced hepatic damage biomarkers in rats. Eur Rev Med Pharmacol Sci. 2015;19:664-70.
  • 27. Chiu YJ, Chou SC, Chiu CS. Hepatoprotective effect of the ethanol extract of Polygonum orientale on carbon tetrachloride-induced acute liver injury in mice. J Food Drug Anal. 2018;26:369-79.
  • 28. Chies AB, Nakazato PCG, Spadella MA Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver. Acta Cir Bras. 2018;33:775-84.
  • 29. Lott J, Wolf P. Alanine and aspartate aminotransferase (ALT and AST). Clinical Enzymology: a case-oriented approach. 1st ed. Chicago:Year Book Medical Publishers;1986. p. 111-138.
  • 30. Akinmoladun AC, Oladejo CO, Josiah SS, Famusiwa CD, Ojo OB, Olaleye MT. Catechin, quercetin and taxifolin improve redox and biochemical imbalances in rotenone-induced hepatocellular dysfunction: Relevance for therapy in pesticide-induced liver toxicity? Pathophysiology 2018;25:365-71.
  • 31. Çimen O, Çimen FK, Gülapoğlu M. The effect of metyrosine on oxidative gastric damage induced by ischemia/reperfusion in rats. Biochemical and histopathological evaluation. Acta Cir Bras. 2018;33:259-67.
  • 32. Baykara B, Tekmen I, Pekcetin C The protective effects of carnosine and melatonin in ischemia-reperfusion injury in the rat liver. Acta Histochem. 2009;111:42-51.

Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury

Year 2019, Volume: 3 Issue: 7, 494 - 497, 29.07.2019
https://doi.org/10.28982/josam.587598

Abstract

Aim:
Ischemia/reperfusion (I/R) procedure applied during liver resection and
transplantation in clinic settings causes liver oxidative damage. The aim of
this study was to examine the effects of taxifolin on liver injury induced by
I/R in rats.

Methods: Albino
Wistar male rats divided into three groups with 6 rats in each group: liver
ischemia-reperfusion (LIR), 50 mg/kg taxifolin + liver ischemia reperfusion
(TLIR) and sham operation (SHAM). An hour before thiopental sodium anesthesia,
50 mg/kg taxifolin was orally administered to the TLIR group and distilled
water to the LIR and SHAM groups. In the TLIR and LIR groups, a clamp was
placed in the hepatic artery, portal vein and bile duct, thereby inducing
ischemia for one hour, and reperfusion for six hours. In the SHAM group, the
abdominal cavity was closed by surgical suture without any procedure. At the
end of this period, rats were sacrificed with high dose anesthesia. Liver
tissues were removed for biochemical and histopathological examinations

 Results: I/R procedure significantly increased
MDA (P<0.001), ALT, AST levels (P<0.001) and decreased tGSH
level (P<0.001) in liver tissue compared to SHAM group. In the taxifolin
treated group, this effect was suppressed. Histopathologically, in the I/R
induced group, pathological findings such as dilated congested blood vessel,
hemorrhage, destruction in the liver parenchyma and edema observed. Liver
tissue in the taxifolin group had near-normal appearance except mild sinusoidal
congestion.







Conclusion: Our results
indicate that taxifolin is an effective agent in reducing hepatic damage caused
by I/R.

References

  • 1. Jeon BR, Yeom DH, Lee SM. Protective effect of allopurinol on hepatic energy metabolism in ischemic and reperfused rat liver. Shock. 2001;15:112-7.
  • 2. Markmann JF. Relaxing liver ischemia reperfusion injury down 1 notch. Am J Transplant. 2018;18:1587-8.
  • 3. Kong R, Gao Y, Sun B. The strategy of combined ischemia preconditioning and salvianolic acid-B pretreatment to prevent hepatic ischemia-reperfusion injury in rats. Dig Dis Sci. 2009;54:2568-76.
  • 4. Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am. 1992;72:65-83.
  • 5. Collard CD, Gelman S. Pathophysiology, clinical manifestations, and prevention of ischemia-reperfusion injury. Anesthesiology 2001;94:1133-8.
  • 6. Zhai Y, Busuttil RW, Kupiec‐Weglinski JW. Liver Ischemia and Reperfusion Injury: New Insights into Mechanisms of Innate—Adaptive Immune‐Mediated Tissue Inflammation. Am J Transplant 2011;11:1563-9.
  • 7. Jaeschke H. Molecular mechanisms of hepatic ischemia-reperfusion injury and preconditioning. Am J Physiol Gastrointest Liver Physiol 2003;284:15-26.
  • 8. Kisaoglu A, Ozogul B, Turan MB Damage induced by paracetamol compared with N-acetylcysteine. J Chin Med Assoc. 2014;77:463-8.
  • 9. Deng WS, Xu Q, Liu Y, Jiang CH, Zhou H, Gu L. Effects of melatonin on liver function and lipid peroxidation in a rat model of hepatic ischemia/reperfusion injury. Exp Ther Med. 2016;11:1955-60.
  • 10. Wang Q, Wang L, Gaiping L, Baoxian Y. A simple and sensitive method for determination of taxifolin on palladium nanoparticles supported poly (diallyldimethylammonium chloride) functionalized graphene modified electrode. Talanta 2017;164:323-9.
  • 11. Topal F, Nar M, Gocer H Antioxidant activity of taxifolin: An activity–structure relationship. J Enzyme Inhib Med Chem. 2016;31:674-83.
  • 12. Gupta MB, Bhalla TN, Gupta GP, Mitra CR, Bhargava KP. Anti-inflammatory activity of taxifolin. Jpn J Pharmacol. 1971;21:377-82.
  • 13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351-8.
  • 14. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968;25:192-205.
  • 15. Halliwell B, Chirico S. Lipid peroxidation: its mechanism, measurement, and significance. Am J Clinl Nutr. 1993;5:715-25.
  • 16. Goulart M, Batoréu MC, Rodrigues AS, Laires A, Rueff J. Lipoperoxidation products and thiol antioxidants in chromium exposed workers. Mutagenesis 2005;20:311-5.
  • 17. Sener MT, Suleyman H, Hacimuftuoglu A. Estimating the postmortem interval by the difference between oxidant/antioxidant parameters in liver tissue. Adv Clin Exp Med. 2012;21:727-33.
  • 18. Demiryilmaz I, Sener E, Cetin N Biochemically and histopathologically comparative review of thiamine’s and thiamine pyrophosphate’s oxidative stress effects generated with methotrexate in rat liver. Med Sci Monit. 2012;18:475-81.
  • 19. Coskun AK, Yigiter M, Oral A The effects of Montelukast on antioxidant enzymes and proinflammatory cytokines on the heart, liver, lungs, and kidneys in a rat model of cecal ligation and puncture–induced sepsis. Sci World J. 2011;11:1341-56.
  • 20. Ingec M, Isaoglu U, Yilmaz M. Prevention of ischemia-reperfusion injury in rat ovarian tissue with the on-off method. J Physiol Pharmacol 2011;62:575-82.
  • 21. Altuner D, Cetin N, Suleyman B Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney. Indian J Pharmacol. 2013;45:339-43.
  • 22. Polat B, Suleyman H, Alp HH. Adaptation of rat gastric tissue against indomethacin toxicity. Chem Biol Interact. 2010;186:82-9.
  • 23. Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 2003;189:41-54.
  • 24. Kisaoglu A, Borekci B, Yapca OE, Bilen H, Suleyman H. Tissue damage and oxidant/antioxidant balance. Eurasian J Med. 2013;45:47-9.
  • 25. Murray RK, Granner DK, Mayes PA, Rodwell VM. Harper's Biochemistry. 25th ed, McGraw-Hill Press: USA; 2000.
  • 26. Yilmaz I, Demiryilmaz I, Turan MI, Cetin N, Gul MA, Suleyman H. The effects of thiamine and thiamine pyrophosphate on alcohol-induced hepatic damage biomarkers in rats. Eur Rev Med Pharmacol Sci. 2015;19:664-70.
  • 27. Chiu YJ, Chou SC, Chiu CS. Hepatoprotective effect of the ethanol extract of Polygonum orientale on carbon tetrachloride-induced acute liver injury in mice. J Food Drug Anal. 2018;26:369-79.
  • 28. Chies AB, Nakazato PCG, Spadella MA Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver. Acta Cir Bras. 2018;33:775-84.
  • 29. Lott J, Wolf P. Alanine and aspartate aminotransferase (ALT and AST). Clinical Enzymology: a case-oriented approach. 1st ed. Chicago:Year Book Medical Publishers;1986. p. 111-138.
  • 30. Akinmoladun AC, Oladejo CO, Josiah SS, Famusiwa CD, Ojo OB, Olaleye MT. Catechin, quercetin and taxifolin improve redox and biochemical imbalances in rotenone-induced hepatocellular dysfunction: Relevance for therapy in pesticide-induced liver toxicity? Pathophysiology 2018;25:365-71.
  • 31. Çimen O, Çimen FK, Gülapoğlu M. The effect of metyrosine on oxidative gastric damage induced by ischemia/reperfusion in rats. Biochemical and histopathological evaluation. Acta Cir Bras. 2018;33:259-67.
  • 32. Baykara B, Tekmen I, Pekcetin C The protective effects of carnosine and melatonin in ischemia-reperfusion injury in the rat liver. Acta Histochem. 2009;111:42-51.
There are 32 citations in total.

Details

Primary Language English
Subjects Surgery
Journal Section Research article
Authors

Huseyin Eken 0000-0001-6095-6002

Eray Kurnaz 0000-0002-9043-558X

Publication Date July 29, 2019
Published in Issue Year 2019 Volume: 3 Issue: 7

Cite

APA Eken, H., & Kurnaz, E. (2019). Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury. Journal of Surgery and Medicine, 3(7), 494-497. https://doi.org/10.28982/josam.587598
AMA Eken H, Kurnaz E. Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury. J Surg Med. July 2019;3(7):494-497. doi:10.28982/josam.587598
Chicago Eken, Huseyin, and Eray Kurnaz. “Biochemical and Histopathological Evaluation of Taxifolin: An Experimental Study in a Rat Model of Liver Ischemia Reperfusion Injury”. Journal of Surgery and Medicine 3, no. 7 (July 2019): 494-97. https://doi.org/10.28982/josam.587598.
EndNote Eken H, Kurnaz E (July 1, 2019) Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury. Journal of Surgery and Medicine 3 7 494–497.
IEEE H. Eken and E. Kurnaz, “Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury”, J Surg Med, vol. 3, no. 7, pp. 494–497, 2019, doi: 10.28982/josam.587598.
ISNAD Eken, Huseyin - Kurnaz, Eray. “Biochemical and Histopathological Evaluation of Taxifolin: An Experimental Study in a Rat Model of Liver Ischemia Reperfusion Injury”. Journal of Surgery and Medicine 3/7 (July 2019), 494-497. https://doi.org/10.28982/josam.587598.
JAMA Eken H, Kurnaz E. Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury. J Surg Med. 2019;3:494–497.
MLA Eken, Huseyin and Eray Kurnaz. “Biochemical and Histopathological Evaluation of Taxifolin: An Experimental Study in a Rat Model of Liver Ischemia Reperfusion Injury”. Journal of Surgery and Medicine, vol. 3, no. 7, 2019, pp. 494-7, doi:10.28982/josam.587598.
Vancouver Eken H, Kurnaz E. Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury. J Surg Med. 2019;3(7):494-7.