Taxifolinin biyokimyasal ve histopatolojik değerlendirilmesi: Rat modelinde karaciğer iskemi reperfüzyon hasarı üzerine deneysel çalışma

Year 2019, Volume: 3 Issue: 7, 494 - 497, 29.07.2019

Huseyin Eken , Eray Kurnaz

https://doi.org/10.28982/josam.587598

Cited By: 2

Abstract

Amaç: Karaciğer rezeksiyonu ve transplantasyon
sırasında uygulanan iskemi reperfüzyon (I/R) prosedürü karaciğerde oksidatif
hasara yol açmaktadır. Bu çalışmanın amacı ratlarda iskemi reperfüzyonun neden olduğu
karaciğer hasarına taxifolinin etkisini incelemektir. Yöntemler: Albino Wistar
erkek ratlar her grupta 6 rat olmak üzere üç gruba ayrıldı: karaciğer iskemi
reperfüzyon (LIR), 50 mg/kg taxifolin + karaciğer iskemi reperfüzyon hasarı
(TLIR) ve sham operasyon (SHAM). Thiopental sodium anestezisinden bir saat önce
TLIR grubuna 50 mg/kg taxifolin; LIR ve SHAM grubuna ise distile su uygulandı.
TLIR ve LIR gruplarında hepatik arter, portal ven ve safra kanalına bir saat
iskemi ve altı saat reperfüzyon oluşturmak üzere klemp yerleştirildi. SHAM
grubunda herhangi bir prosedür uygulanmadan karın boşluğu cerrahi dikişle
kapatıldı. Bu periyodun sonunda ratlar yüksek doz anestezi ile sakrifiye
edildi. Karaciğer dokuları biyokimyasal ve histopatolojik inceleme için
çıkarıldı. I/R prosedüründe SHAM grubu ile kıyaslandığında karaciğer dokusunda
MDA (P<0,001), AST, ALT (P<0,001) değerlerinde anlamlı artış
ve tGSH (P<0,001) değerinde azalma
görüldü. Taxifolin uygulanan grupta bu etki baskılandı.

Bulgular: I/R grubunda patolojik olarak dilate
konjeste kan damarları, hemoraji, karaciğer parankiminde destrüksiyon ve ödem
gözlendi. Taxifolin uygulanan grupta karaciğer dokusu orta derecede sinusoidal
konjesyon dışında normale yakın görünümdeydi.

Sonuç: Sonuçlarımız taxifolinin iskemi reperfüzyon
sonrası oluşan karaciğer hasarını önlemede etkili olduğunu göstermektedir.

Keywords

İskemi, Reperfüzyon, Rat, Taxifolin

Biochemical and histopathological evaluation of taxifolin: An experimental study in a rat model of liver ischemia reperfusion injury

Abstract

Aim:
Ischemia/reperfusion (I/R) procedure applied during liver resection and
transplantation in clinic settings causes liver oxidative damage. The aim of
this study was to examine the effects of taxifolin on liver injury induced by
I/R in rats.

Methods: Albino
Wistar male rats divided into three groups with 6 rats in each group: liver
ischemia-reperfusion (LIR), 50 mg/kg taxifolin + liver ischemia reperfusion
(TLIR) and sham operation (SHAM). An hour before thiopental sodium anesthesia,
50 mg/kg taxifolin was orally administered to the TLIR group and distilled
water to the LIR and SHAM groups. In the TLIR and LIR groups, a clamp was
placed in the hepatic artery, portal vein and bile duct, thereby inducing
ischemia for one hour, and reperfusion for six hours. In the SHAM group, the
abdominal cavity was closed by surgical suture without any procedure. At the
end of this period, rats were sacrificed with high dose anesthesia. Liver
tissues were removed for biochemical and histopathological examinations

 Results: I/R procedure significantly increased
MDA (P<0.001), ALT, AST levels (P<0.001) and decreased tGSH
level (P<0.001) in liver tissue compared to SHAM group. In the taxifolin
treated group, this effect was suppressed. Histopathologically, in the I/R
induced group, pathological findings such as dilated congested blood vessel,
hemorrhage, destruction in the liver parenchyma and edema observed. Liver
tissue in the taxifolin group had near-normal appearance except mild sinusoidal
congestion.

Conclusion: Our results
indicate that taxifolin is an effective agent in reducing hepatic damage caused
by I/R.

Keywords

Ischemia, Reperfusion, Rat, Taxifolin

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