Eco-friendly Approach for Silver Nanoparticles Synthesis from Lemon Extract and their Anti-oxidant, Anti-bacterial, and Anti-cancer Activities

An Erratum to this article was published on May 31, 2023. https://dergipark.org.tr/en/pub/jotcsa/article/1286431

Abstract

To create silver nanoparticles, researchers use bionanotechnology techniques because they are economical and environmentally friendly. The current study shows that lemon juice (Citrus-limon) can be used to biosynthesize silver nanoparticles (Ag NPs). The synthesized silver nanoparticles have been characterized by the surface plasmon resonance (SPR) measured at λmax = 430 nm, confirming the formation of AgNPs. Moreover, Fourier Transform Infrared (FTIR) analysis was carried out to identify possible bio-molecules responsible for the bio-reduction of silver ions. The x-ray diffraction (XRD) peaks at (111, 200, 220, 222, and 311) confirm the found face-centered cubic (FCC) crystal structure of AgNPs in solution. Transmission-Electron-Microscopy (TEM) images showed that AgNPs have spherical morphology with sizes ranging from 10-50 nm. Furthermore, the Particles Size Analyzer (PSA) confirmed these sizes and ranges. Synthesized AgNPs have high anti-oxidant activity according to the (scavenging of DPPH radicals, total anti-oxidant, and reducing power) assays. Also, the anti-bacterial activity of AgNPs was evaluated by a well diffusion method, and the results suggest that they are more sensitive to gram-positive bacteria than gram-negative ones, with the average diameter of the inhibition zones for AgNPs ranging from 4.11 to 25.87 mm and 1.38 to 22.3 mm against S. aureus and E. coli bacteria, respectively. In vitro studies of AgNPs against MCF-7 breast cancer cell s lines showed a good cytotoxic effect p<0.05 with an IC50 value of 47 μg/mL; this study could be beneficial for nanotechnology-based pharmaceutical and biomedical applications.    

Keywords

• Silver nano-particles
• Citrus limon extract
• Anti-oxidant activity
• Anti-bacterial activity
• Anti-cancer activity.

References

1. 1. Kumar B, Smita K, Cumbal L, Debut A, Pathak RN. Sonochemical Synthesis of Silver Nanoparticles Using Starch: A Comparison. Bioinorganic Chemistry and Applications. 2014;2014:1–8.
2. 2. Mohammed RS, Aadim KA, Ahmed KA. Synthesis of CuO/ZnO and MgO/ZnO Core/Shell Nanoparticles with Plasma Gets and Study of their Structural and Optical Properties. Journal of Modern Science. 2022;8(2):9.
3. 3. Jakinala P, Lingampally N, Hameeda B, Sayyed RZ, Khan M. Y, Elsayed EA, et al. Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential. Kumar P, editor. PLoS ONE. 2021 Mar 18;16(3):e0241729.
4. 4. Ahmed RH, Mustafa DE. Green synthesis of silver nanoparticles mediated by traditionally used medicinal plants in Sudan. Int Nano Lett. 2020 Mar;10(1):1–14.
5. 5. Niluxsshun MCD, Masilamani K, Mathiventhan U. Green Synthesis of Silver Nanoparticles from the Extracts of Fruit Peel of Citrus tangerina, Citrus sinensis, and Citrus limon for Antibacterial Activities. Ciccarella G, editor. Bioinorganic Chemistry and Applications. 2021 Feb 2;2021:1–8.
6. 6. Choudhury R, Majumder M, Roy DN, Basumallick S, Misra TK. Phytotoxicity of Ag nanoparticles prepared by biogenic and chemical methods. Int Nano Lett. 2016 Sep;6(3):153–9.
7. 7. Pirtarighat S, Ghannadnia M, Baghshahi S. Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. J Nanostruct Chem. 2019 Mar;9(1):1–9.
8. 8. Vasyliev G, Vorobyova V, Skiba M, Khrokalo L. Green Synthesis of Silver Nanoparticles Using Waste Products (Apricot and Black Currant Pomace) Aqueous Extracts and Their Characterization. Advances in Materials Science and Engineering. 2020 Jul 13;2020:1–11.

9. 9. Yazdanian M, Rostamzadeh P, Rahbar M, Alam M, Abbasi K, Tahmasebi E, et al. The Potential Application of Green-Synthesized Metal Nanoparticles in Dentistry: A Comprehensive Review. De Matteis V, editor. Bioinorganic Chemistry and Applications. 2022 Mar 3;2022:1–27.
10. 10. Vankar PS, Shukla D. Biosynthesis of silver nanoparticles using lemon leaves extract and its application for antimicrobial finish on fabric. Appl Nanosci. 2012 Jun;2(2):163–8.
11. 11. Vinson JA, Su X, Zubik L, Bose P. Phenol Antioxidant Quantity and Quality in Foods: Fruits. J Agric Food Chem. 2001 Nov 1;49(11):5315–21.
12. 12. Selvam SI, Joicesky SMB, Dashli AA, Vinothini A, Premkumar K. Assessment of anti bacterial, anti inflammation and wound healing activity in Wistar albino rats using green silver nanoparticles synthesized from Tagetes erecta leaves. JANS. 2021 Mar 14;13(1):343–51.
13. 13. Pandey S, Oza G, Vishwanathan M, Sharon M. Biosynthesis of highly stable gold nanoparticles using Citrus limone. Ann Biol Res. 2012;3(5):2378–82.
14. 14. Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J, Srinivasan K. Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2011 Aug;79(3):594–8.
15. 15. Rai A, Chaudhary M, Ahmad A, Bhargava S, Sastry M. Synthesis of triangular Au core–Ag shell nanoparticles. Materials Research Bulletin. 2007 Jul;42(7):1212–20.
16. 16. Linh DHT, Anh NP, Mi TTA, Tinh NT, Cuong HT, Quynh TL, et al. Biosynthesis, Characteristics and Antibacterial Activity of Silver Nanoparticles Using Lemon Citrus Latifolia Extract. Mater Trans. 2018 Sep 1;59(9):1501–5.
17. 17. Prathna TC, Chandrasekaran N, Raichur AM, Mukherjee A. Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids and Surfaces B: Biointerfaces. 2011 Jan;82(1):152–9.
18. 18. Biv K, Nolan R. Green Synthesis of Silver Nanoparticles using Lemon Extract, Characterization, and Antimicrobial Properties [Internet]. Poster presentation presented at: Undergraduate Posters; 2021 May; Salem, Massachusetts.
19. 19. Mosae Selvakumar P, Antonyraj CA, Babu R, Dakhsinamurthy A, Manikandan N, Palanivel A. Green Synthesis and Antimicrobial Activity of Monodispersed Silver Nanoparticles Synthesized Using Lemon Extract. Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry. 2016 Feb 1;46(2):291–4.
20. 20. Niraimathi KL, Sudha V, Lavanya R, Brindha P. Biosynthesis of silver nanoparticles using Alternanthera sessilis (Linn.) extract and their antimicrobial, antioxidant activities. Colloids and Surfaces B: Biointerfaces. 2013 Feb;102:288–91.
21. 21. Hawar SN, Al-Shmgani HS, Al-Kubaisi ZA, Sulaiman GM, Dewir YH, Rikisahedew JJ. Green Synthesis of Silver Nanoparticles from Alhagi graecorum Leaf Extract and Evaluation of Their Cytotoxicity and Antifungal Activity. Omri A, editor. Journal of Nanomaterials. 2022 Jan 5;2022:1–8.
22. 22. Gondwal M, Joshi nee Pant G. Synthesis and Catalytic and Biological Activities of Silver and Copper Nanoparticles Using Cassia occidentalis. International Journal of Biomaterials. 2018;2018:1–10.
23. 23. Mohamed NH, Ismail MA, Abdel-Mageed WM, Mohamed Shoreit AA. Antimicrobial activity of latex silver nanoparticles using Calotropis procera. Asian Pacific Journal of Tropical Biomedicine. 2014 Nov;4(11):876–83.
24. 24. Villaño D, Fernández-Pachón MS, Moyá ML, Troncoso AM, García-Parrilla MC. Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta. 2007 Jan 15;71(1):230–5.
25. 25. Prieto P, Pineda M, Aguilar M. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry. 1999 May;269(2):337–41.
26. 26. Singh R, Singh S, Kumar S, Arora S. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food and Chemical Toxicology. 2007 Jul;45(7):1216–23.
27. 27. Mulvaney P. Surface Plasmon Spectroscopy of Nanosized Metal Particles. Langmuir. 1996 Jan 1;12(3):788–800.
28. 28. Jinu U, Gomathi M, Saiqa I, Geetha N, Benelli G, Venkatachalam P. Green engineered biomolecule-capped silver and copper nanohybrids using Prosopis cineraria leaf extract: Enhanced antibacterial activity against microbial pathogens of public health relevance and cytotoxicity on human breast cancer cells (MCF-7). Microbial Pathogenesis. 2017 Apr;105:86–95.
29. 29. Wanjari AK, Patil MP, Chaudhari UE, Gulhane VN, Kim GD, Kiddane AT. Bactericidal and photocatalytic degradation of methyl orange of silver-silver chloride nanoparticles synthesized using aqueous phyto-extract. Particulate Science and Technology. 2022 Nov 17;40(8):1033–40.
30. 30. Pourmortazavi SM, Taghdiri M, Makari V, Rahimi-Nasrabadi M. Procedure optimization for green synthesis of silver nanoparticles by aqueous extract of Eucalyptus oleosa. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2015 Feb;136:1249–54.
31. 31. Iftikhar M, Zahoor M, Naz S, Nazir N, Batiha GES, Ullah R, et al. Green Synthesis of Silver Nanoparticles Using Grewia optiva Leaf Aqueous Extract and Isolated Compounds as Reducing Agent and Their Biological Activities. Yin J, editor. Journal of Nanomaterials. 2020 Oct 24;2020:1–10.
32. 32. Tyagi PK, Tyagi S, Gola D, Arya A, Ayatollahi SA, Alshehri MM, et al. Ascorbic Acid and Polyphenols Mediated Green Synthesis of Silver Nanoparticles from Tagetes erecta L. Aqueous Leaf Extract and Studied Their Antioxidant Properties. Tapia Hernández JA, editor. Journal of Nanomaterials. 2021 Aug 2;2021:1–9.
33. 33. Udayasoorian C, Kumar KV, Jayabalakrishnan M. Extracellular synthesis of silver nanoparticles using leaf extract of Cassia auriculata. Dig J Nanomater Biostruct. 2011;6(1):279–83.
34. 34. Göl F, Aygün A, Seyrankaya A, Gür T, Yenikaya C, Şen F. Green synthesis and characterization of Camellia sinensis mediated silver nanoparticles for antibacterial ceramic applications. Materials Chemistry and Physics. 2020 Aug;250:123037.
35. 35. Kharabi Masooleh A, Ahmadikhah A, Saidi A. Green synthesis of stable silver nanoparticles by the main reduction component of green tea ( Camellia sinensis L.). IET nanobiotechnol. 2019 Apr;13(2):183–8.
36. 36. Ali S, Jalal M, Ahmad H, Sharma D, Ahmad A, Umar K, et al. Green Synthesis of Silver Nanoparticles from Camellia sinensis and Its Antimicrobial and Antibiofilm Effect against Clinical Isolates. Materials. 2022 Oct 8;15(19):6978.
37. 37. Chandra A, Bhattarai A, Yadav AK, Adhikari J, Singh M, Giri B. Green Synthesis of Silver Nanoparticles Using Tea Leaves from Three Different Elevations. ChemistrySelect. 2020 Apr 16;5(14):4239–46.
38. 38. Gomaa EZ. Antimicrobial, antioxidant and antitumor activities of silver nanoparticles synthesized by Allium cepa extract: A green approach. Journal of Genetic Engineering and Biotechnology. 2017 Jun;15(1):49–57.
39. 39. Es-haghi A, Javadi F, Taghavizadeh Yazdi ME, Amiri MS. The Expression of Antioxidant Genes and Cytotoxicity of Biosynthesized Cerium Oxide Nanoparticles Against Hepatic Carcinoma Cell Line. Avicenna J Med Biochem. 2019 Jun 25;7(1):16–20.
40. 40. Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci. 2008 Jun;4(2):89–96.
41. 41. M G, Dj M, Vinaykiya V, V B, Dutta S, Pawar R, et al. Screening of Antibacterial and Antioxidant Activity of Biogenically Synthesized Silver Nanoparticles from Alternaria alternata, Endophytic Fungus of Dendrophthoe falcata-a Parasitic Plant. BioNanoSci. 2022 Mar;12(1):128–41.
42. 42. Taha ZK, Hawar SN, Sulaiman GM. Extracellular biosynthesis of silver nanoparticles from Penicillium italicum and its antioxidant, antimicrobial and cytotoxicity activities. Biotechnol Lett. 2019 Sep;41(8–9):899–914.
43. 43. Khane Y, Benouis K, Albukhaty S, Sulaiman GM, Abomughaid MM, Al Ali A, et al. Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties. Nanomaterials. 2022 Jun 10;12(12):2013.
44. 44. Alavi M, Karimi N. Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract. Artificial Cells, Nanomedicine, and Biotechnology. 2017 Dec 12;1–16.
45. 45. Samreen FG, Muzaffar R, Nawaz M, Gul S, Basra MAR. Synthesis, Characterization and Anti-Microbial Activity of Citrus limon Mediated Nanoparticles [Internet]. LIFE SCIENCES; 2018 Nov [cited 2023 Feb 5].
46. 46. Link MP, Goorin AM, Miser AW, Green AA, Pratt CB, Belasco JB, et al. The Effect of Adjuvant Chemotherapy on Relapse-Free Survival in Patients with Osteosarcoma of the Extremity. N Engl J Med. 1986 Jun 19;314(25):1600–6.
47. 47. Prasad KS, Pathak D, Patel A, Dalwadi P, Prasad R, Patel P, et al. Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. African Journal of Biotechnology. 2011;10(41):8122.
48. 48. Verma A, Mehata MS. Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity. Journal of Radiation Research and Applied Sciences. 2016 Jan;9(1):109–15.
49. 49. Babu RH, Yugandhar P, Savithramma N. Synthesis, characterization and antimicrobial studies of bio silica nanoparticles prepared from Cynodon dactylon L.: a green approach. Bull Mater Sci. 2018 Jun;41(3):65.
50. 50. Patil MP, Rokade AA, Ngabire D, Kim GD. Green Synthesis of Silver Nanoparticles Using Water Extract from Galls of Rhus Chinensis and Its Antibacterial Activity. J Clust Sci. 2016 Sep;27(5):1737–50.
51. 51. Paul B, Bhuyan B, Purkayastha DD, Dhar SS. Photocatalytic and antibacterial activities of gold and silver nanoparticles synthesized using biomass of Parkia roxburghii leaf. Journal of Photochemistry and Photobiology B: Biology. 2016 Jan;154:1–7.
52. 52. Khan SS, Mukherjee A, Chandrasekaran N. Studies on interaction of colloidal silver nanoparticles (SNPs) with five different bacterial species. Colloids and Surfaces B: Biointerfaces. 2011 Oct;87(1):129–38.
53. 53. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science. 2004 Jul;275(1):177–82.
54. 54. Patil SV, Borase HP, Patil CD, Salunke BK. Biosynthesis of Silver Nanoparticles Using Latex from Few Euphorbian Plants and Their Antimicrobial Potential. Appl Biochem Biotechnol. 2012 Jun;167(4):776–90.
55. 55. Patil MP, Kim GD. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Appl Microbiol Biotechnol. 2017 Jan;101(1):79–92.
56. 56. Majeed S, Danish M, Zakariya NA, Hashim R, Ansari MT, Alkahtani S, et al. In Vitro Evaluation of Antibacterial, Antioxidant, and Antidiabetic Activities and Glucose Uptake through 2-NBDG by Hep-2 Liver Cancer Cells Treated with Green Synthesized Silver Nanoparticles. Srivastava S, editor. Oxidative Medicine and Cellular Longevity. 2022 May 17;2022:1–14.
57. 57. Ovais M, Khalil AT, Raza A, Khan MA, Ahmad I, Islam NU, et al. Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics. Nanomedicine. 2016 Dec;11(23):3157–77.
58. 58. Banerjee PP, Bandyopadhyay A, Nagesh H, Policegoudra R, Bhattacharya S, Karak N, et al. Mentha arvensis (Linn.)-mediated green silver nanoparticles trigger caspase 9-dependent cell death in MCF7 and MDA-MB-231 cells. BCTT. 2017 Apr;Volume 9:265–78.
59. 59. Soshnikova V, Kim YJ, Singh P, Huo Y, Markus J, Ahn S, et al. Cardamom fruits as a green resource for facile synthesis of gold and silver nanoparticles and their biological applications. Artificial Cells, Nanomedicine, and Biotechnology. 2018 Jan 2;46(1):108–17.
60. 60. Mohammadinejad R, Moosavi MA, Tavakol S, Vardar DÖ, Hosseini A, Rahmati M, et al. Necrotic, apoptotic and autophagic cell fates triggered by nanoparticles. Autophagy. 2019 Jan 2;15(1):4–33.
61. 61. Barabadi H, Vahidi H, Damavandi Kamali K, Rashedi M, Saravanan M. Antineoplastic Biogenic Silver Nanomaterials to Combat Cervical Cancer: A Novel Approach in Cancer Therapeutics. J Clust Sci. 2020 Jul;31(4):659–72.
62. 62. Hembram KC, Kumar R, Kandha L, Parhi PK, Kundu CN, Bindhani BK. Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent. Artificial Cells, Nanomedicine, and Biotechnology. 2018 Nov 12;46(sup3):38–51.