Isolation and Characterization of Cholest-5-en-3-ol Compound from the Ethyl Acetate Extract of Stem Bark Ulin Plant (Eusideroxylon zwageri Teijm & Binn)

Abstract

Cholest-5-en-3-ol is an organic compound that belongs to the category of steroidal secondary metabolites. The compound has a double bond and a hydroxyl group and is commonly found in plants that contain oils such as coconut, nuts, and others. So far, there have been no reports of isolating the compound cholest-5-en-3-ol from the extract of the stem bark of the ulin plant. This compound has been found in very small amounts. A maceration method was used to obtain the extract. The isolation method used for the isolation of the compound was chromatography, while for the elucidation of the structure of the compound, UV-Vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance spectroscopy (NMR) were used. The ethyl acetate extract is the most abundant with 85.5 g. The isolation results were obtained as a white needle-like solid. Based on the spectra of UV-Vis spectrophotometry, FTIR spectra, and chemical shifts of NMR, the elucidated compound is the cholest-5-en-3-ol.

Keywords

• Isolation
• Characterization
• Ulin
• Cholest-5-en-3-ol.

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