Effect of 3-Amino-1,2,4-Triazole-5-Carboxylic Acid on Human Blood Erythrocyte Catalase

Year 2024, , 541 - 546, 15.05.2024

Kübra Çıkrıkcı , Nahit Gencer

https://doi.org/10.18596/jotcsa.1381362

Abstract

Catalase is an antioxidant enzyme with great therapeutic potential that scavenges hydrogen peroxide, a reactive oxygen species produced during cellular metabolism. Substances containing 1,2,4-triazole structures are biologically important heterocyclic compounds found in the structure of many pharmaceutical drugs used in drug discovery studies against various types of diseases in the human body. In this study, the effect of phosphate buffer prepared at different pHs and 3-amino-1,2,4-triazole-5-carboxylic acid (ATZc) on catalase enzyme activity in human blood erythrocytes was determined. It was determined that the catalase enzyme was inhibited by ATZc at different pH levels. The weakest inhibition was observed at pH 5.5 (IC50:49.01 µM), whereas the strongest inhibition was observed at pH 7.5 (IC50:23.21 µM).

Keywords

3-amino-1;2;4-triazole-5-carboxylic acid, Catalase, enzyme inhibition

References

• 1. Yuriy K, Kusdemir G, Volodymyr P, Tüzün B, Taslimi P, Karatas OF, et al. A biochemistry‐oriented drug design: synthesis, anticancer activity, enzymes inhibition, molecular docking studies of novel 1,2,4-triazole derivatives. J Biomol Struct Dyn. 2023 Sept;1-17. Available from: <URL>
• 2. Nasri S, Bayat M, Kochia K. Strategies for synthesis of 1,2,4-triazole-containing scaffolds using 3-amino-1,2,4-triazole. Mol Divers. 2022 Feb;26(1):717-39. Available from: <URL>
• 3. Tian G, Song Q, Liu Z, Guo J, Cao S, Long S. Recent advances in 1,2,3- and 1,2,4-triazole hybrids as antimicrobials and their SAR: A critical review. Eur J Med Chem. 2023 Nov;259:115603. Available from: <URL>
• 4. Xu D, Wu L, Yao H, Zhao L. Catalase‐Like Nanozymes: Classification, Catalytic Mechanisms, and Their Applications. Small. 2022 Sept;18(37):2203400. Available from: <URL>
• 5. Shahraki S, Shiri F, Razmara Z. Improving enzymatic performance of antioxidant enzyme catalase in combination with [Mn (phen) 2 Cl.H 2 O]Cl.tu complex. Appl Organomet Chem. 2023 Apr;37(4):e7061. Available from: <URL>
• 6. Margoliash E, Schejter A. Kinetics of the Irreversible Inhibition of Catalase by 3-Amino-1,2,4-triazole in the Presence of Hydrogen Peroxide and Catalase-Hydrogen Peroxide Complex I Hydrogen Donors. J Biol Chem.1962 Jly;237(7):2359-63. Available from: <URL>
• 7. Koohshekan B, Divsalar A, Saiedifar M, Saboury AA, Ghalandari B, Gholamian A, et al. Protective effects of aspirin on the function of bovine liver catalase: A spectroscopy and molecular docking study. J Mol Liq. 2016 Jun;218:8-15. Available from: <URL>
• 8. Quds R, Iqbal Z, Arif A, Mahmood R. Mancozeb-induced cytotoxicity in human erythrocytes: enhanced generation of reactive species, hemoglobin oxidation, diminished antioxidant power, membrane damage and morphological changes. Pestic Biochem Physiol. 2023 Jun;193:105453. Available from: <URL>
• 9. Aebi H, Suter H, Feinstein RN. Activity and stability of catalase in blood and tissues of normal and acatalasemic mice. Biochem. Genet. 1968 Aug;2:245-251. Available from: <URL>
• 10. Grytsai O, Valiashko O, Penco-Campillo M, Dufies M, Hagege A, Demange L, et al. Synthesis and biological evaluation of 3-amino-1,2,4-triazole derivatives as potential anticancer compounds. Bioorganic Chem. 2020 Nov;104:104271.<URL>
• 11. Küçükgüzel ŞG, Çıkla-Süzgün P. Recent advances bioactive 1,2,4-triazole-3-thiones. Eur J Med Chem. 2015 Jun;97:830-70. Available from: <URL>
• 12. Akhtar T, Zafar W, Sumrra S. A Review on the Assessment of Enzyme Inhibition Activity by 1, 2, 4-Triazole Derivatives (Review). South J of Res. 2022 Jly;2(2):189-203. Available from: <URL>
• 13. Galasso M, Gambino S, Romanelli MG, Donadelli M, Scupoli MT. Browsing the oldest antioxidant enzyme: catalase and its multiple regulation in cancer. Free Radic Biol Med. 2021 Aug;172:264-72. Available from: <URL>
• 14. Costa SA, Tzanov T, Filipa Carneiro A, Paar A, Gübitz GM, Cavaco-Paulo A. Studies of stabilization of native catalase using additives. Enzyme Microb Technol. 2002 Mar;30(3):387-91. Available from: <URL>
• 15. Goyal MM, Basak A. Human catalase: looking for complete identity. Protein Cell. 2010 Oct;1(10):888-97. Available from: <URL>
• 16. Aydemir T, Kuru K. Purification and partial characterization of catalase from chicken erythrocytes and the effect of various inhibitors on enzyme activity. Turk J Chem. 2003;27(1):85-97. Available from:<URL>
• 17. Pontes RB, Colombari DSA, De Paula PM, Colombari E, Andrade CAF, De Luca LA, et al. Sympathetic and angiotensinergic activity in spontaneously hypertensive rats treated with 3-amino-1,2,4-triazole. Auton Neurosci. 2023 Sept;248:103107. Available from: <URL>
• 18. Tada S, Hatano M, Nakayama Y, Volrath S, Cuyer D, Ward E, and Ohta D. lnsect Cell Expression of Recombinant lmidazoleglycerolphosphate Dehydratase of Arabidopsis and heat and lnhibition by Triazole Herbicides. Plant Physiol. 1995;109:153-159. Available from: <URL>