Characterization, Antioxidant and Anticholinesterase Activity of Compounds Isolated from Alkaloid Extracts of Citrus aurantifolia Root Bark

Year 2024, , 1227 - 1236, 30.08.2024

Djélé Alette Edwige Zıale , Demel Axel Adou , Kohue Christelle Chantal N'gaman-kouassı , Julia Deschamp , Nadia Bouchemal , Tony Lionel Palama , Marc Lecouvey , Janat Akhanovna Mamyrbekova-békro , Yves-alain Bekro

https://doi.org/10.18596/jotcsa.1395690

Abstract

Xanthyletin (1), edulinin (2), 1-methyl-2-[(E)-(prop-1-enyl)]quinolin-4-one (3), 1,2-dimethylquinolin-4-one (4), lonchocarpol A (5), grandisinin (6), citracridone-I (7) and 5-hydroxynoracronycin (8) were isolated from alkaloid extracts of the root bark of Citrus aurantifolia. Their structures were characterized using spectral data (NMR, HRMS, and IR). Furthermore, compounds 2, 3, 4, and 6 known in the literature have been isolated for the first time in Citrus aurantifolia. However, compounds 1, 5, 7 and 8 are already known from the plant studied. The concentration required to reduce 50% of free radicals (RC50) was carried out. Compounds 3 (RC50= 185.836 µmol/L) and 4 (RC50= 218.277 µmol/L) compared with vitamin C (RC50= 17.033 µmol/L) showed antioxidant efficacy. The highest anticholinesterase activity was observed for compound 6 at 21.129 µM followed by compounds 3 and 4 at 251.130, 287.208 µM, respectively.

Keywords

Citrus aurantifolia, alkaloids, isollation, antioxidant, anticholinesterase

Thanks

The authors thank the "Ministère de l'Enseignement Supérieur et de la Recherche Scientifique de Côte d'Ivoire (MESRSCI) for the financial support given to Djélé Alette Edwige Zialé for her PhD training at the Laboratoire CSPBAT of the Université Sorbonne Paris Nord, Bobigny (France). They also express their gratitude to the USPN for the access to the NMR Platform and to Professor Malan Djah François for the identification of the plant species studied.

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