Biogenic Copper Oxide Nanoparticles Synthesized from Whole Plant Extract of Nicotiana plumbaginifolia Viv.: Characterization, Antibacterial, and Antioxidant Properties

Year 2024, , 1005 - 1016, 30.08.2024

Abhimanyu Pawar , Arvind Mungole , Kıshor Naktode

https://doi.org/10.18596/jotcsa.1422924

Abstract

Nanoparticles crafted through biological processes show potential for advancing medicine. Plant-derived compounds, produced through environmentally friendly green synthesis, present distinctive and beneficial applications in the field of nanomedicine. This study describes an easy, sustainable, environmentally friendly, and cost-efficient method to create copper oxide nanoparticles (CuO NPs) using whole part of Nicotiana plumbaginifolia Viv. extract. The characterization involved various techniques like solid UV-Visible-DR analysis, Fourier transform infrared (FTIR), EDAX analysis, X-ray diffraction (XRD), transmitted electron microscopy (TEM), and scanning electron microscopy (SEM). The copper oxide nanoparticles (CuO NPs) were found to be quasi-spherical pattern, with sizes ranging from 12 to 14 nm, and exhibited a crystal structure identified as monoclinic. The resulting copper oxide nanoparticles (CuO NPs) were examined for antimicrobial and antioxidant properties. It showed suppressing bacterial growth against tested human pathogenic bacteria, emphasizing their potential as antimicrobial agents. Results revealed that the maximum zone of inhibition was observed when the concentrations (25, 50, and 100 μL,) of NPs is increased against S. aureus i.e. 17 mm, 20 mm and 22 mm respectively. Whereas findings also reveal potent antioxidant activity, with escalating CuO nanoparticle concentrations correlating to increased percentage inhibition 50 μg/mL – 1.68%, 100 μg/mL – 10.45%, 150 μg/mL – 18.54%, 200 μg/mL – 37.83%, and 250 μg/mL – 51.72%. The highest activity, at 51.72%, occurs at 250 μg/mL.

Keywords

Biogenic synthesis, CuO nanoparticles, Antimicrobial activity, Antioxidant activity

Thanks

We extend heartfelt appreciation to the principal of Nevjabai Hitkarini College, Bramhapuri, Maharashtra (India), for steadfast support, encouragement, and providing research facilities. Our gratitude also extends to the Sophisticated Test and Instrumentation Centre (STIC) at Cochin University of Science and Technology, Cochin, Kerala, India, for their invaluable assistance in characterizing the samples.

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