Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity

Rana Alhakimi; Zerrin Zerenler Çalışkan

doi:10.18596/jotcsa.1770080

Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity

Abstract

Lipases are versatile green biocatalysts from the hydrolase family, valued for their high selectivity, specificity, and catalytic efficiency in diverse industrial applications, including pharmaceuticals, food, and bioenergy. Their activity and productivity are strongly influenced by a range of physicochemical and operational factors. This review summarizes the key parameters affecting lipase performance, including temperature, pH, metal ions, nitrogen sources, surfactants, agitation speed, and incubation time. The roles of substrate-related factors-such as concentration, derivative type, substrate ratio, and enzyme loading-are also described. In addition, recent methods and emerging techniques aimed at enhancing lipase activity and reducing product losses, such as lipase engineering, immobilization, bioimprinting, interfacial imprinting, the use of conventional organic co-solvents, additives, natural deep eutectic solvents, and multi-lipase systems, are outlined. By compiling and organizing current knowledge, this work serves as a practical reference for optimizing lipase-based processes in both research and industrial contexts.

Keywords

Thanks

The authors gratefully acknowledge the support of Yildiz Technical University and access to relevant databases.

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Review

Authors

Rana Alhakimi
0009-0001-1617-1520
Türkiye

Zerrin Zerenler Çalışkan ^*
0000-0003-0010-1744
Türkiye

Publication Date

January 30, 2026

Submission Date

August 21, 2025

Acceptance Date

December 4, 2025

Published in Issue

Year 2026 Number: 2026-1

DOI

https://doi.org/10.18596/jotcsa.1770080

IZ

https://izlik.org/JA74SY66JT

Cite

RIS / Bibtex

APA

Alhakimi, R., & Zerenler Çalışkan, Z. (2026). Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity. Journal of the Turkish Chemical Society Section A: Chemistry, 2026-1, 1-15. https://doi.org/10.18596/jotcsa.1770080

AMA

1.Alhakimi R, Zerenler Çalışkan Z. Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity. JOTCSA. 2026;(2026-1):1-15. doi:10.18596/jotcsa.1770080

Chicago

Alhakimi, Rana, and Zerrin Zerenler Çalışkan. 2026. “Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity”. Journal of the Turkish Chemical Society Section A: Chemistry, no. 2026-1: 1-15. https://doi.org/10.18596/jotcsa.1770080.

EndNote

Alhakimi R, Zerenler Çalışkan Z (January 1, 2026) Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity. Journal of the Turkish Chemical Society Section A: Chemistry 2026-1 1–15.

IEEE

[1]R. Alhakimi and Z. Zerenler Çalışkan, “Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity”, JOTCSA, no. 2026-1, pp. 1–15, Jan. 2026, doi: 10.18596/jotcsa.1770080.

ISNAD

Alhakimi, Rana - Zerenler Çalışkan, Zerrin. “Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity”. Journal of the Turkish Chemical Society Section A: Chemistry. 2026-1 (January 1, 2026): 1-15. https://doi.org/10.18596/jotcsa.1770080.

JAMA

1.Alhakimi R, Zerenler Çalışkan Z. Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity. JOTCSA. 2026;:1–15.

MLA

Alhakimi, Rana, and Zerrin Zerenler Çalışkan. “Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity”. Journal of the Turkish Chemical Society Section A: Chemistry, no. 2026-1, Jan. 2026, pp. 1-15, doi:10.18596/jotcsa.1770080.

Vancouver

1.Rana Alhakimi, Zerrin Zerenler Çalışkan. Physicochemical Parameters Affecting Lipase Performance: Including New Methods and Techniques Increasing Lipase Activity. JOTCSA. 2026 Jan. 1;(2026-1):1-15. doi:10.18596/jotcsa.1770080