Anticancer Activity of Papaver Somniferum

Abstract

This work describes the pharmacological activity of extracts of Papaver somniferum, a poppy species. P. somniferum products are still considered as a unique source of drug for many diseases. The present study was designed to determine antiproliferative and cytotoxic effects of P. somniferum extracts on HeLa (Human Cervix Carcinoma), HT29 (Human Colorectal Adenocarcinoma), C6 (Rat Brain Tumor Cells), and Vero (African Green Monkey Kidney) cell lines. Alkaloids-rich extracts of P. somniferum exhibited antiproliferative effects on various cancer cell lines, especially at high concentrations. We assessed the ability of extracts of P. somniferum to harm the membrane of the cells. Results indicated that P. somniferum extracts destroy cellular membrane in tumor cell lines at high concentrations. Remarkably, the LDH test results disclosed that cytotoxicity of P. somniferum on cells was low at mid concentrations. This may indicate its cytostatic potential. The results of this study support the efficacy of P. somniferum extracts as an anticancer agent.   

References

1. World Cancer Report 2014. http://www.iarc.fr/en/publications/books/wcr/wcr-order.php (accessed 16.04.16).
2. Elgorashi E. Screening of medicinal plants used in South African traditional medicine for genotoxic effects. Toxicol. Lett. 2003; 143, 195–207. doi:10.1016/S0378-4274(03)00176-0.
3. Fennell CW, Lindsey KL, McGaw LJ, Sparg SG, Stafford GI, Elgorashi EE, Grace OM, van Staden J. Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. J. Ethnopharmacol. 2004; 94, 205–217.
4. Sithranga Boopathy N, Kathiresan K. Anticancer Drugs from Marine Flora: An Overview. J. Oncol. 2010; 1–18. doi:10.1155/2010/214186.
5. Weerapreeyakul N, Nonpunya A, Barusrux S, Thitimetharoch T, Sripanidkulchai B. Evaluation of the anticancer potential of six herbs against a hepatoma cell line Chin. Med. 2012; 7, 15.
6. Lee CC, Houghton P. Cytotoxicity of plants from Malaysia and Thailand used traditionally to treat cancer. J. Ethnopharmacol. 2005; 100, 237–243.
7. Berrington D, Lall N. Anticancer Activity of Certain Herbs and Spices on the Cervical Epithelial Carcinoma (HeLa) Cell Line. Evidence-Based Complement. Altern. Med. 2012; 1–11.
8. Itharat A, Houghton PJ, Eno-Amooquaye E, Burke P, Sampson JH, Raman A. In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. J. Ethnopharmacol. 2004; 90, 33–38.

9. Gürkök T, Parmaksız İ, Boztepe G, Kaymak E. Alkaloid biosynthesis Mechanism in Opium Poppy (Papaver somniferum L.). Electronic Journal of BioTechnology 2010; 1(2) 31-45.
10. Parmaksiz I, Özcan S. Morphological, chemical and molecular analyses of Turkish Papaver accessions (Sect. Oxytona). Turk J. Bot. 2011; 35, 1-16.
11. Gürkök T, Kaymak E, Boztepe G, Koyuncu M, Parmaksız İ. Molecular characterization of the genus Papaver section Oxytona using ISSR markers. Turk J. Bot. 2013; 37:644-650.
12. Chen X, Dang T-TT, Facchini PJ. Noscapine comes of age. Phytochemistry 2015; 111, 7–13.
13. Priyadarshani A, Chuttani K, Mittal G, Bhatnagar A. Radiolabeling, biodistribution and gamma scintigraphy of noscapine hydrochloride in normal and polycystic ovary induced rats. J. Ovarian Res. 2010; 3, 10.
14. Mahmoudian M, Rahimi-Moghaddam P. The anti-cancer activity of noscapine: a review. Recent Pat. Anticancer. Drug Discov. 2009; 4, 92–7.
15. Takeuchi R, Hoshijima H, Onuki N, Nagasaka H, Chowdhury SA, Kawase M, Sakagami H. Effect of anticancer agents on codeinone-induced apoptosis in human cancer cell lines. Anticancer Res. 2005; 25, 4037–41.
16. Sueoka E, Sueoka N, Kai Y, Okabe S, Suganuma M, Kanematsu K, Yamamoto T, Fujiki H. Anticancer activity of morphine and its synthetic derivative, KT-90, mediated through apoptosis and inhibition of NF-kappaB activation. Biochem. Biophys. Res. Commun. 1998; 252, 566–70.
17. Porstmann T, Ternynck T, Avrameas S. Quantitation of 5-bromo-2-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of the lymphoid cell proliferative response. J Immunol Methods 1985; 82(1): 169-79. doi:10.1016/0022-1759(85)90236-4.
18. Segal MS, Goldstein MM, Attinger EO. The use of noscapine (narcotine) as an antitussive agent. CHEST Journal. 1957; 32(3), 305-9.
19. Joshi HC, Zhou J. Noscapine and analogues as potential chemotherapeutic agents. Drug News Perspect. 2000;13(9), 543-6.
20. Anderson JT, Ting AE, Boozer S, Brunden KR, Danzig J, Dent T. Discovery of S-phase arresting agents derived from noscapine. J. Med. Chem. 2005; 48, 2756–2758.
21. Aneja R, Vangapandu SN, Lopus M, Chandra R, Panda D, Joshi HC. Development of a novel nitro-derivative of noscapine for the potential treatment of drug-resistant ovarian cancer and T-cell lymphoma. Mol. Pharmacol. 2006; 69, 1801–1809.
22. Boke H, Ozhuner E, Turktas M, Iskender I, Sebahattin O, Unver T. Regulation of the alkaloid biosynthesis by miRNA in opium poppy. Plant Biotech. 2015; 1-12. DOI: 10.1111/pbi.12346.
23. Gurkok T, Turktas M, Parmaksiz I, Unver T. Transcriptome Profiling of Alkaloid Biosynthesis in Elicitor Induced Opium Poppy. Plant Mol. Biol. Report. 2015; 33, 673–688.
24. Facchini PJ, De Luca V. Opium poppy and Madagascar periwinkle: model non-model systems to investigate alkaloid biosynthesis in plants. Plant J. 2008; 54, 763–784.
25. Alagöz Y, Gürkök T, Parmaksız İ, Ünver T. Identification and sequence analysis of alkaloid biosynthesisgenes in Papaver section Oxytona. TURKISH J. Biol. 2016; 40, 174–183.
26. Gurkok T, Ozhuner E, Parmaksiz I, Özcan S, Turktas M, İpek A, Demirtas I, Okay S, Unver T. Functional Characterization of 4′OMT and 7OMT Genes in BIA Biosynthesis. Front. Plant Sci. 2016; 7.