Optimization of Hydrophilic-Lipophilic Balance Solid-Phase Extraction of Phthalates in Pharmaceutical Preparations

Year 2019, , 271 - 280, 20.10.2019

Pelin Köseoğlu Yılmaz

https://doi.org/10.18596/jotcsa.526124

Cited By: 2

Abstract

Phthalates,
which are used as plasticizers to soften rough polymers in the industrial
processes, possess endocrine system disrupting activities. In this study, a hydrophilic-lipophilic
balance solid-phase extraction method was optimized for seven phthalates as
dimethyl, dipropyl, benzyl butyl, di-n-butyl, dicyclohexyl, di-(2-ethylhexyl)
and di-n-octyl phthalates in terms of the type and the volume of the
conditioning and elution solvents. The phthalates were quantified by a
validated HPLC/UV method. The recovery values were higher than 80% for
dimethyl, dipropyl, benzylbutyl, di-n-butyl and dicyclohexyl phthalates. Using
the optimized conditions three types of liquid pharmaceutical preparations as an
intravenous isotonic sodium chloride solution, an intravenous dextrose solution
and an osmotic laxative kept in polymeric packages were analyzed with high
accuracy, precision and recovery. Only the intravenous isotonic sodium chloride
solution was determined to be contaminated with dipropyl phthalate at a
concentration of 13.2 μg/L.

Keywords

Hydrophilic-lipophilic balance, high performance liquid chromatography, pharmaceutical, phthalate, solid-phase extraction

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