Research Article
BibTex RIS Cite
Year 2020, , 37 - 42, 15.02.2020
https://doi.org/10.18596/jotcsa.628615

Abstract

References

  • 1. Kalaycıoğlu Z., Erim FB., Total phenolic contents, antioxidant activities, and bioactive ingredients of juices pomegranate cultivars worldwide, Food Chemistry, 2017; 221; 496-507.
  • 2. TÜİK, Turkish Statistical Institute,2019, http://tuik.gov.tr
  • 3. Adhami VM., Mukhtar H., Polyphenols from green tea and pomegranate for prevention prostate cancer, Free Radic. Res. 2006; 40; 1095-104.
  • 4. Colombo E., Sangiovanni E., Dell’Agli M., A review on the anti-inflammatory activity of pomegranate in the gastrointestinal tract., Evidence-Based Complementary and Alternative Medicine, 2013; 1-12.
  • 5. Lansky EP., Newman RA., Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer, Journal of Ethnopharmacology, 2007; 109; 177-206.
  • 6. Sharma P., McCless .F., Afaq F., Pomegranate for prevention and treatment of cancer: an update, Molecules, 2017; 22(1); 177.
  • 7. Williams AR., Soelberg J., Jäger AK., Anthelmentic properties of traditional African and Carribbean medicinal plants: identification of extracts with potential activity against Ascaris suum in vitro, Parasite, 2016; 23(24);1-6.
  • 8. Nadia Z., Aicha M., Sihem H., Abdelmalik B., In vivo analgesic activities and safety assesment of Vitis vinifera L and Punica granatum L fruits extracts, Tropical Journal of Pharmaceutical Research, 2017; 16(3); 553-61.
  • 9. Bassiri-Jahromi S., Doostkam A., Comparative evaluation of bioactive compounds of various cultivars of pomegranate (Punica granatum) in different world regions, AIMS Agriculture and Food, 2019; 4(1); 41-55.
  • 10. DaSilva NA., Nahar PP., Ma H., Eid A., Wei Z., Meschwitz S., Zawia NH., Slitt AL., Seeram NP., Pomegranate ellagitannin-gut microbial-derived metabolites, urolithins, inhibit neuroinflammation in vitro, Nutritional Neuroscience, 2019; 22(3); 185-95.
  • 11. Sharayei P., Azarpazhooh E., Zomorodi S., Ramaswamy HS., Ultrasound assisted extraction of bioactive compounds from pomegranate (Punica granatum L.) peel, LWT, 2019; 101; 342-50.
  • 12. Moghaddam G., Sharifzadeh M., Hassanzadeh G., Khanavi M., Dolatshahi F., Sadeghi N., Oveisi MR., Hajimahmoodi M., Anti-ulcerative potential of Punica granatum L. (Lythraceace) hydroalcohol fruit peel extract,Tropical Journal of Pharmaceutical Research, 2014; 13(7); 1093-7.
  • 13. Zhao S-S., Ma D-X., Zhu Y., Zhao J-H., Zhang Y., Chen J-Q., Sheng Z-L., Antidiarrheal effect of bioactivity-guided fractions and bioactive components of pomegranate (Punica granatum L.) peels, Neurogastroenterology & Motility, 2018; 30(7); 1-10.
  • 14. Bekir J., Cazaux S., Mars M., Bouajila J., In vitro anti-cholinesterase and anti-hyperglycemic activities of flowers extracts from seven pomegranate varieties, Industrial Crops and Products, 2016; 81; 176-9.
  • 15. Wu S., Tian L., A new flavone glucoside together with known ellagitannins and flavones with anti-diabetic and anti-obesity activities from the flowers of pomegranate (Punica granatum), Natural Product Research, 2019; 33(2); 252-7.
  • 16. Abdolahi N., Soltani A., Mirzaali A., Soltani S., Balakheyli H., Aghaei M., Antibacterial and antioxidant activities and phytochemical properties of Punica granatum flowers in Iran, Iranian Journal of Science and Technology, Transactions A: Science, 2018; 42; 1105-10.
  • 17. Huang THW., Peng G., Kota BP., Li GQ., Yamahara J., Roufogalis BD., Li Y., Anti-diabetic action of Punica granatum flower extract: Activation of PPAR-γ and identification of an active component, Toxicology and Applied Pharmacology, 2005; 207(2); 160-9.
  • 18. Bekir J., Mars M., Vicendo P., Fterrich A., Bouajila J., Chemical composition and antioxidant, anti-inflammatory and antiproliferation activities of pomegranate (Punica granatum) flowers, Journal of Medicinal Food, 2013; 16(6); 544-50.
  • 19. Singleton VL., Rossi JA., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, American Journal of Enology and Viticulture, 1965; 16; 144-58.
  • 20. Zhishen J., Mengcheng T., Jianming W., The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals, Food Chemistry, 1999; 64(4); 555-9.
  • 21. Blois MS., Antioxidant determinations by the use of a stable free radical, Nature, 1958; 181; 1199-200.
  • 22. Oyaizu M., Studies on product of browning reaction prepared from glucose amine, Japanese Journal of Nutrition, 1986; 44; 307-15.
  • 23. Dinis TCP., Madeira VMC., Almeida LM., Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Archives of Biochemistry and Biophysics, 1994; 315(1); 161-169.
  • 24. Eddebbagh M., Messaoudi M., Abourriche A., Berrada M., Attaleb M, Benbacer L., Bennamara A., Correlation of the cytotoxic and antioxidant activities of Moroccan pomegranate (Punica Granatum) with phenolic and flavonoid contents, Journal of Pharmacy and Pharmacology, 2016; 4; 511-9.
  • 25. Rashid M., Shafi S., Evaluation of in vitro antioxidant and anti-diabetic potential of Kashmiri pomegranate (Punica granatum Linn.) flower extract, International Research Journal of Pharmacy, 2018; 9(9); 117-24.
  • 26. Elfellah W., Hannachi H., Tlili N., Yahia Y., Nasri N., Ferchichi A., Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower, Journal of Medicinal Plants Research, 2012; 6; 4724-30.

Evaluation of phytochemical contents and antioxidant activity of pomegranate flower

Year 2020, , 37 - 42, 15.02.2020
https://doi.org/10.18596/jotcsa.628615

Abstract




Pomegranate is one of the most abundant
fruit consumed in Turkey. This study aimed to determine the content of total
phenolic, total flavonoid and antioxidant activity of Punica granatum L.
flower in different extracts. Antioxidant activities of different extracts were
determined 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging, reducing
power and metal chelating methods. The result showed that, total phenolic
content for the extracts was ranged from 14.82 to 90.86 mg gallic acid
equivalents(GAE) / g extract. The contents of flavonoids were found to range
from 7.35 to 500.00 mg quercetin equivalents (QUE)/ g extract. All pomegranate
flower extracts displayed remarkable antioxidant activity according to DPPH and
reducing power assays. Especially the methanolic extract of pomegranate flower
possesses significant scavenging activity against DPPH• (85.80 %), as well as
the largest contents of
flavonoids
and phenolic compounds. The antioxidant capacity of the methanolic extract was
also greater than those of BHT and α-tocopherol in DPPH and reducing power
assays. The results demonstrated that the antioxidant activity of extracts of Punica
granatum
L. flower might be, at least in part, due to the existence of high
content of flavonoids compounds and other phenolics.  

References

  • 1. Kalaycıoğlu Z., Erim FB., Total phenolic contents, antioxidant activities, and bioactive ingredients of juices pomegranate cultivars worldwide, Food Chemistry, 2017; 221; 496-507.
  • 2. TÜİK, Turkish Statistical Institute,2019, http://tuik.gov.tr
  • 3. Adhami VM., Mukhtar H., Polyphenols from green tea and pomegranate for prevention prostate cancer, Free Radic. Res. 2006; 40; 1095-104.
  • 4. Colombo E., Sangiovanni E., Dell’Agli M., A review on the anti-inflammatory activity of pomegranate in the gastrointestinal tract., Evidence-Based Complementary and Alternative Medicine, 2013; 1-12.
  • 5. Lansky EP., Newman RA., Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer, Journal of Ethnopharmacology, 2007; 109; 177-206.
  • 6. Sharma P., McCless .F., Afaq F., Pomegranate for prevention and treatment of cancer: an update, Molecules, 2017; 22(1); 177.
  • 7. Williams AR., Soelberg J., Jäger AK., Anthelmentic properties of traditional African and Carribbean medicinal plants: identification of extracts with potential activity against Ascaris suum in vitro, Parasite, 2016; 23(24);1-6.
  • 8. Nadia Z., Aicha M., Sihem H., Abdelmalik B., In vivo analgesic activities and safety assesment of Vitis vinifera L and Punica granatum L fruits extracts, Tropical Journal of Pharmaceutical Research, 2017; 16(3); 553-61.
  • 9. Bassiri-Jahromi S., Doostkam A., Comparative evaluation of bioactive compounds of various cultivars of pomegranate (Punica granatum) in different world regions, AIMS Agriculture and Food, 2019; 4(1); 41-55.
  • 10. DaSilva NA., Nahar PP., Ma H., Eid A., Wei Z., Meschwitz S., Zawia NH., Slitt AL., Seeram NP., Pomegranate ellagitannin-gut microbial-derived metabolites, urolithins, inhibit neuroinflammation in vitro, Nutritional Neuroscience, 2019; 22(3); 185-95.
  • 11. Sharayei P., Azarpazhooh E., Zomorodi S., Ramaswamy HS., Ultrasound assisted extraction of bioactive compounds from pomegranate (Punica granatum L.) peel, LWT, 2019; 101; 342-50.
  • 12. Moghaddam G., Sharifzadeh M., Hassanzadeh G., Khanavi M., Dolatshahi F., Sadeghi N., Oveisi MR., Hajimahmoodi M., Anti-ulcerative potential of Punica granatum L. (Lythraceace) hydroalcohol fruit peel extract,Tropical Journal of Pharmaceutical Research, 2014; 13(7); 1093-7.
  • 13. Zhao S-S., Ma D-X., Zhu Y., Zhao J-H., Zhang Y., Chen J-Q., Sheng Z-L., Antidiarrheal effect of bioactivity-guided fractions and bioactive components of pomegranate (Punica granatum L.) peels, Neurogastroenterology & Motility, 2018; 30(7); 1-10.
  • 14. Bekir J., Cazaux S., Mars M., Bouajila J., In vitro anti-cholinesterase and anti-hyperglycemic activities of flowers extracts from seven pomegranate varieties, Industrial Crops and Products, 2016; 81; 176-9.
  • 15. Wu S., Tian L., A new flavone glucoside together with known ellagitannins and flavones with anti-diabetic and anti-obesity activities from the flowers of pomegranate (Punica granatum), Natural Product Research, 2019; 33(2); 252-7.
  • 16. Abdolahi N., Soltani A., Mirzaali A., Soltani S., Balakheyli H., Aghaei M., Antibacterial and antioxidant activities and phytochemical properties of Punica granatum flowers in Iran, Iranian Journal of Science and Technology, Transactions A: Science, 2018; 42; 1105-10.
  • 17. Huang THW., Peng G., Kota BP., Li GQ., Yamahara J., Roufogalis BD., Li Y., Anti-diabetic action of Punica granatum flower extract: Activation of PPAR-γ and identification of an active component, Toxicology and Applied Pharmacology, 2005; 207(2); 160-9.
  • 18. Bekir J., Mars M., Vicendo P., Fterrich A., Bouajila J., Chemical composition and antioxidant, anti-inflammatory and antiproliferation activities of pomegranate (Punica granatum) flowers, Journal of Medicinal Food, 2013; 16(6); 544-50.
  • 19. Singleton VL., Rossi JA., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, American Journal of Enology and Viticulture, 1965; 16; 144-58.
  • 20. Zhishen J., Mengcheng T., Jianming W., The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals, Food Chemistry, 1999; 64(4); 555-9.
  • 21. Blois MS., Antioxidant determinations by the use of a stable free radical, Nature, 1958; 181; 1199-200.
  • 22. Oyaizu M., Studies on product of browning reaction prepared from glucose amine, Japanese Journal of Nutrition, 1986; 44; 307-15.
  • 23. Dinis TCP., Madeira VMC., Almeida LM., Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers, Archives of Biochemistry and Biophysics, 1994; 315(1); 161-169.
  • 24. Eddebbagh M., Messaoudi M., Abourriche A., Berrada M., Attaleb M, Benbacer L., Bennamara A., Correlation of the cytotoxic and antioxidant activities of Moroccan pomegranate (Punica Granatum) with phenolic and flavonoid contents, Journal of Pharmacy and Pharmacology, 2016; 4; 511-9.
  • 25. Rashid M., Shafi S., Evaluation of in vitro antioxidant and anti-diabetic potential of Kashmiri pomegranate (Punica granatum Linn.) flower extract, International Research Journal of Pharmacy, 2018; 9(9); 117-24.
  • 26. Elfellah W., Hannachi H., Tlili N., Yahia Y., Nasri N., Ferchichi A., Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower, Journal of Medicinal Plants Research, 2012; 6; 4724-30.
There are 26 citations in total.

Details

Primary Language English
Subjects Analytical Chemistry
Journal Section Articles
Authors

F. Zehra Küçükbay 0000-0002-7180-9486

Zehra Tekin 0000-0003-3682-3044

Publication Date February 15, 2020
Submission Date October 2, 2019
Acceptance Date October 13, 2019
Published in Issue Year 2020

Cite

Vancouver Küçükbay FZ, Tekin Z. Evaluation of phytochemical contents and antioxidant activity of pomegranate flower. JOTCSA. 2020;7(1):37-42.