Chemical compounds, antioxidant properties and antimicrobial activity of olive leaves derived volatile oil in West Anatolia

Year 2021, , 511 - 518, 31.05.2021

Nilüfer Vural , Mehmet Abdulkadir Akay

https://doi.org/10.18596/jotcsa.833139

Cited By: 4

Abstract

In this article, the chemical compounds, antimicrobial and antioxidant activity of the volatile oil from leaves of Olea europaea L. cultivar from Turkey (Ayvalık) has been studied. The essential oil was provided with a Clevenger apparatus and analyzed by GC-MS/FID. This analysis leads to the detection of 42 compounds representing 99.59±1.15% of the total oil. The major constituents were a-pinene (9.82±0.33%), benzylalcohol (8.83±0.27%), phenethylalcohol (8.52±0.25%), 2-monopalmitin (8.13±0.28%), palmitic acid (5.53±0.41%), octadecanoic acid 2,3-dihydroxypropylester (5.84±0.42%), phytol (4.22±0.17%), and benzaldehyde (4.21±0.38%). The antimicrobial activities of the dried leaves essential oils were assessed against seven bacterial and four fungal strains. Significantly, the essential oil has an efficient antibacterial activity toward to the bacterial strains such as Bacillus cereus ATCC 14579, Candida albicans ATCC 10231, Enterococcus faecalis ATCC 29212 and Klebsiella pneumoniae ATCC 13883. The olive a leaf essential oils showed significant antimicrobial and antioxidant effects. This study gives more knowledge for the development of this crucial therapeutic plant.

Keywords

Olive leaves volatiles, GC-MS/FID, Chemical composition, Antimicrobial activity, Antioxidant activity

References

• 1. Brahmi F, Flamini G, Issaoui M, Dhibi M, Dabbou S, Mastouri M, Hammami M. Chemical composition and biological activities of volatile fractions from three Tunisian cultivars of olive leaves. Medicinal Chemistry Research. 2012; 21(10): 2863-72. Doi: 10.1007/s00044-011-9817-8
• 2. Borges A, Jose H, Homem V, Simoes M. Comparison of techniques and solvents on the antimicrobial and antioxidant potential of extracts from Acacia dealbata and Olea europaea. Antibiotics. 2020; 9(2): 48. Doi:10.3390/antibiotics9020048
• 3. Debib A, Boukhatem MN. Phenolic content, antioxidant and antimicrobial activities of “Chemlali” olive leaf (Olea europaea L.) extracts. International Journal of Pharmacology, Phytochemistry and Ethnomedicine. 2017; 6:38-46.Doi:10.18052/www.scipress.com/IJPPE. 6.38.
• 4. Vural N, Algan Cavuldak Ö, Akay MA. D-Optimal design and multi-objective optimization for green extraction conditions developed with ultrasonic probe for oleuropein. Journal of Applied Research on Medicinal and Aromatic Plants. 2021; 20: 100279. Doi: 10.1016/j.jarmap.2020.100279
• 5. Difonzo G, Squeo G, Pasqualone A, Summo C, Paradiso VM, Caponio F. The challenge of exploiting polyphenols from olive leaves: addition to foods to improve their shelf‐life and nutritional value. Journal of the Science of Food and Agriculture. 2020. Doi: 10.1002/jsfa.10986
• 6. Nicolì F, Negro C, Vergine M, Aprile A, Nutricati E, Sabella E, Miceli A, Luvisi A, De Bellis L. Evaluation of phytochemical and antioxidant properties of 15 Italian Olea europaea L. Cultivar Leaves. Molecules. 2019; 24(10): 1998. Doi: 10.3390/molecules24101998
• 7. Chiaino E, Micucci M, Cosconati S, Novellino E, Budriesi R, Chiarini A, Frosini M. Olive leaves and Hibiscus flowers extracts-based preparation protect brain from oxidative stress-induced injury. Antioxidants. 2020; 9(9):806. Doi: 10.3390/antiox9090806
• 8. Samouh Y, Lemrani A, Mimouni H, Mohamad J, Said AAH. Ethnopharmacological Study of Herbal Medicines used to treat Cancer in Morocco. J Phytopharmacol 2019; 8(3):135-41. Doi:10.31254/phyto.2019.8309
• 9. Castillo JJ, Alcaraz M, Benavente-Garcia O. Antioxidant and radioprotective effects of olive leaf extract. Olives and olive oil in Health and Disease Prevention. 2010; 12: 951-8. Doi: 10.1016/B978-0-12-374420-3.00102-9
• 10. Venditti A, Serrilli AM, Rizza L, Frasca G, Cardile V, Bonina FP, Bianco A. Aromadendrine, a new component of the flavonoid pattern of Olea europaea L. and its anti-inflammatory activity. Natural Product Research. 2013; 27(4):340-9. Doi: 10.1080/14786419.2012.693924
• 11. Khayyal MT, El-Ghazaly MA, Abdallah DM,.Nassar NN, Okpanyi SN, Kreuter MH. Blood pressure lowering effect of an olive leaf extract (Olea europaea) in L-NAME induced hypertension in rats. Arzneimittel-Forschung/Drug Research, 2002; 52(11):797–802.
• 12. Agalias A, Melliou E, Magiatis P, Mitaku S, Gikas E, Tsarbopoulos A. Quantitation of oleuropein and related metabolites in decoctions of Olea leaves from ten Greek cultivated varieties by HPLC with diode array detection (HPLC-DAD). Journal of Liq. Chr. & Related Tech. 2005; 28: 1557-71. Doi: 10.1081/JLC-200058355
• 13. Merrouni IA, Elachouri M. Anticancer medicinal plants used by Moroccan people: Ethnobotanical, preclinical, phytochemical and clinical evidence. Journal of Ethnopharmacology. 2021; 266:113435. Doi:10.1016/j.jep.2020.113435
• 14.Özcan MM, Matthäus B. A review: benefit and bioactive properties of olive (Olea europaea L.) leaves. European Food Research and Technology. 2017; 243(1): 89-99. Doi: 10.1007/s00217-016-2726-9
• 15. Çilingiroğlu S. Evaluation of coronary artery anomalies with angiography in Turkish adult population. Turkiye Klinikleri J Cardiovasc Sci. 2009; 21(3):363-9.
• 16. Upadhyay RK. Evaluation of antibacterial and antifungal activities of olive (Olea europaea) essential oil. International Journal of Green Pharmacy. 2014; 8(3): 180-6. Doi: 10.22377/ijgp.v8i3.382
• 17. Adams RP. Identification of essential oil components by Gas Chromatography/Quadrupole Mass Spectroscopy. 4th ed. Allured Publishing Corp, Carol Stream, IL. ISBN-10:1932633219. 2007.
• 18. Gil MI, Tomás-Barberán F A, Hess-Pierce B, Kader AA. Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. Journal of Agricultural and Food Chemistry. 2002; 50(17): 4976–82. Doi:10.1021/jf020136b 
• 19. Vardar-Ünlü G, Ünlü M, Dönmez E, Vural N. Chemical composition and in vitro antimicrobial activity of the essential oil of Origanum minutiflorum O Schwarz & PH Davis. Journal of the Science of Food and Agriculture. 2007; 87(2): 255-9. Doi: 10.1002/jsfa.2708
• 20. Upadhyay RK. GC-MS analysis and in vitro antimicrobial susceptibility of Foeniculum vulgare seed essential oil. American Journal of Plant Sciences. 2015; 6: 1058-68. Doi: 10.4236/ajps.2015.67110.
• 21. NCCLS (National Committee for Clinical Laboratory Standards). Performance Standards for Antimicrobial Susceptibility Testing, 9th International Supplement, M100-S9, Wayne, PA. 1999.
• 22. Ünlü M, Ergene E, Ünlü G V, Zeytinoglu H. S, Vural N. Composition, antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum Blume (Lauraceae). Food and Chemical Toxicology. 2010; 48(11): 3274-80. Doi: 10.1016/j.fct.2010.09.001
• 23. SAS/STAT® 9.1, SAS Institute, SAS User’s Guide. SAS Institue Inc.,Cary, NC. 2004.
• 24. Campeol E, Flamini G, Chericoni S, Catalano S, Cremonini R. Volatile compounds from three cultivars of Olea europaea from Italy. Journal of Agricultural and Food Chemistry. 2001; 49(11): 5409-11. Doi: 10.1021/jf010455n
• 25. Campeol E, Flamini G, Cioni P. L, Morelli I, Cremonini R, Ceccarini L. Volatile fractions from three cultivars of Olea europaea L. collected in two different seasons. Journal of Agriculture Food Chem. 2003; 51: 1994−9. Doi: 10.1021/jf026025u
• 26. Haloui E, Marzouk Z, Marzouk B, Bouftira I, Bouraoui A, Fenina N. 2010. Pharmacological activities and chemical composition of the Olea europaea L. leaf essential oils from Tunisia. Journal of Food Agriculture & Environment. 8(2): 204-8.
• 27.Brahmi F, Flamini G, Mechri B, Dhibi M, Hammami M. Antioxidant activity of the leaf volatile oil and extracts of Olea europaea L.cv. Chetoui from N. Tunisia. Journal of Agricultural and Food Chemistry. 2015; 4(3):1-5. Doi: 10.4172/2324-9323.1000171
• 28. Boukhebti H, Chaker AN, Lograda T, Ramdani M. Chemical and antimicrobial properties of essential oils of Olea europea L. International Journal of Pharmacology & Toxicology. 2015; 5(1): 42-6.
• 29. Keskin D, Uğur A, Ceyhan N, Dbeys AD. Antimicrobial activity and chemical constitutions of West Anatolian olive (Olea europaea L.) leave. Journal of Food, Agriculture & Environment. 2012; 10(2): 99-102.