Year 2016, Volume 3 , Issue 3, Pages 131 - 144 2017-01-08

Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds

Nimet Bölgen [1] , Didem Demir [2] , Fatma Öfkeli [3] , Seda Ceylan [4]

Polymeric scaffolds produced by cryogelation technique have attracted increasing attention for tissue engineering applications. Cryogelation is a technique which enables to produce interconnected porous matrices from the frozen reaction mixtures of polymers or monomeric precursors. Chitosan is a biocompatible, biodegradable, nontoxic, antibacterial, antioxidant and antifungal natural polymer that is obtained by deacetylation of chitin, which is mostly found in the exoskeleton of many crustacean. In this study, chitin was isolated from the exoskeleton of blue crap (Callinectes sapidus) using a chemical method. Callinectes sapidus samples were collected from a market, as a waste material after it has been consumed as food. Demineralization, deproteinization and decolorization steps were applied to the samples to obtain chitin. Chitosan was prepared from isolated chitin by deacetylation at high temperatures. The chemical compositon of crab shell, extracted chitin and chitosan were characterized with FTIR analyses. And also to determine the physicochemical and functional properties of the produced chitosan; solubility, water binding and fat binding analysis were performed. Chitosan cryogel scaffolds were prepared by crosslinking reaction at cryogenic conditions at constant amount of chitosan (1%, w/v) with different ratios of glutaraldehyde (1, 3, and 6%, v/v) as crosslinker. The chemical structure of the scaffolds were examined by FTIR. Also, the water uptake capacity of scaffolds have been determined. Collectively, the results suggested that the characterized chitosan cryogels can be potential scaffolds to be used in tissue engineering applications.
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Journal Section Articles

Author: Nimet Bölgen

Author: Didem Demir

Author: Fatma Öfkeli

Author: Seda Ceylan


Application Date : July 4, 2016
Acceptance Date : January 18, 2021
Publication Date : January 8, 2017

Vancouver Bölgen N , Demir D , Öfkeli F , Ceylan S . Extraction and Characterization of Chitin and Chitosan from Blue Crab and Synthesis of Chitosan Cryogel Scaffolds. Journal of the Turkish Chemical Society Section A: Chemistry. 2017; 131-144.