DETERMINATION OF COCAINE AND BENZOYLECGONINE IN BIOLOGICAL MATRICES BY HPLC AND LC-MS/MS.

Year 2016, Volume: 3 Issue: 3, 535 - 550, 08.01.2017

Zinar Pinar Gumus , Veysel Umut Celenk Emine Guler Bilal Demir Hakan Coskunol Suna Timur

https://doi.org/10.18596/jotcsa.82665

Cited By: 3

Abstract

Cocaine is a powerfully addictive illicit drug. Cocaine abuse and addiction continue to increase in the world. Most analytical tests for the detection of cocaine use include the analysis of the metabolite, benzoylecgonine, in the urine. Benzoylecgonine is a major urinary metabolite of cocaine. Generally, the most common biologic matrices used for the analysis of cocaine contain the urine and blood however saliva was also added as a matrix in this study. Practical, quick, reliable, precise and accurate, reproducible analytical methods have been developed and validated for cocaine and benzoylecgonine. In addition, this chromatographic techniques were used as both initial test and confirmatory. The validated chromatographic method was successfully applied to the analysis of cocaine and benzoylecgonine compounds in synthetic biological matrix. Confirmation analyses were made by LC-MS/MS to support reliability of HPLC results. As a result of this study, it can be claimed that HPLC could be a good alternative for the analyses of various biological matrices in forensic studies. Also the matrices and concentrations were determined by HPLC instrument could be used where necessary.

Keywords

cocaine, benzoylecgonine, HPLC, LC-MS/MS

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