Year 2017, Volume 4 , Issue 1, Pages 133 - 148 2017-01-09

The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels

Kadir Erol [1]

The separation and purification methods for the isolation of an important biomolecule calmoduline protein is extremely important. Among these methods, the adsorption technique is extremely popular, and the cryogels as adsorbents with the macro porous structure and interconnected flow channels cryogel they have are preferred in this field. In this study, the adsorption of calmoduline via Ca(II) immobilized poly (2-hydroxyethyl methacrylate-glycidyl methacrylate), poly (HEMA-GMA), cryogels through changing interaction time, calmoduline initial concentration and temperature conditions. For the characterization of cryogels, the swelling test, Fourier Transform Infrared (FT-IR) Spectroscopy, Scanning Electron Microscopy (SEM), surface area (BET), elemental analysis and ICP-OES methods were performed. Nicotinamide molecule was used as Ca (II) chelating agent and the adsorption capacity of the cryogels was estimated as 1.812 mg calmoduline / g cryogel. The adsorption models of the adsorption reaction were examined by the Langmuir and Freundlich isotherm models and was determined to be more appropriate for Langmuir isotherm model.

Adsorption, calmoduline; cryogel; nicotinamide.
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Journal Section Articles

Author: Kadir Erol


Application Date : September 28, 2016
Acceptance Date : July 10, 2020
Publication Date : January 9, 2017

Vancouver Erol K . The Adsorption of Calmoduline via Nicotinamide Immobilized Poly(HEMA-GMA) Cryogels. Journal of the Turkish Chemical Society Section A: Chemistry. 2017; 133-148.