Evaluation of Total Polyphenol Content, Antioxidant Activity and Chemical Composition of Methanolic Extract from Allium Kharputense Freyn et. Sint. and Determination of Mineral and Trace Elements

Year 2017, Volume: 4 Issue: 3, 691 - 708, 01.07.2017

Erdal Yabalak , Ahmet Murat Gizir

https://doi.org/10.18596/jotcsa.315094

Cited By: 12

Abstract

Allium
kharputense Freyn Et. Sint. (A. kharputense) was analyzed for total polyphenol
content, antioxidant activity, chemical composition and mineral and trace
element composition. Total polyphenol content was determined as 257 mg GA 100 g^-1
dried weight by using Folin-Ciocalteu method. DPPH test was performed for antioxidant
activity analysis. IC₅₀ values of gallic acid and Trolox were
determined as 0.02642 mg mL^-1 and 0.225 mg mL^-1,
respectively. IC₅₀ value of A.
kharputense was found as 2.186 mg mL^-1. DPPH free
radical-scavenging activity of extract from 1 mg of A. kharputense was determined 0.01207 mg GAE and 0.1029 mg TrE,
respectively. GC-MS was used to determine chemical composition. Dimethyl
trisulfide, methyl (methylthio)methyl disulfide, 2-Pyrrolidinone,
4-vinylphenol, 2-Phenylpyrrole,
norharmane, linoleic acid, palmitic
acid, γ-Tocopherol and α-Tocopherol are some of 40 compounds which were
detected in methanolic extracts of A.
Kharputense. 20 Mineral and trace elements were determined along with their
levels by ICP-MS using microwave digestion procedure for preparing acid extracts.

Keywords

Antioxidant activity, Allium Kharputense Freyn Et. Sint, Polyphenolic content, DPPH

References

• Firat M. The Ethnobotanical Usage of Some East Anatolian. Manas J Agric Life Sci. 2015; 5(1): 80-6.
• Block E. The Chemistry of Garlic and Onions. Sci Am. 1985; 252(3): 114-8.
• Hanen N, Fattouch S, Ammar E, Neffati M. Antimicrobial potentials of Allium roseum: Recent Advances and Trends. Sci against Microb Pathog Commun Curr Res Technol Adv. 2011; 2(1): 1205-10.
• Demirtas I, Erenler R, Elmastas M, Goktasoglu A. Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chem. 2013; 136(1): 34-40.
• Reuter HD. Allium sativum and Allium ursinum: Part 2 pharmacology and medicinal application. Phytomedicine. Gustav Fischer Verlag, Struttgart • Jena • New York; 1995; 2(1): 73-91.
• Fritsch RM, Keusgen M. Occurrence and taxonomic significance of cysteine sulphoxides in the genus Allium L. (Alliaceae). Phytochemistry. 2006; 67(11): 1127-35.
• Panomket P, Wanram S, Srivorasmas T, Pongprom N. Bioactivity of plant extracts against Burkholderia pseudomallei. Asian Biomed. 2012; 6(4):619-23.
• Corea G, Fattorusso E, Lanzotti V. Saponins and flavonoids of Allium triquetrum. J Nat Prod. 2003; 66(11): 1405-11.
• Kimbaris AC, Siatis NG, Daferera DJ, Tarantilis PA, Pappas CS, Polissiou MG. Comparison of distillation and ultrasound-assisted extraction methods for the isolation of sensitive aroma compounds from garlic (Allium sativum). Ultrason Sonochem. 2006; 13(1): 54-60.
• Banerjee SK, Mukherjee PK, Maulik SK. Garlic as an antioxidant: The good, the bad and the ugly. Phyther Res. 2003; 17(2): 97-106.
• Erdogan EA, Yabalak E, Everest A, Gizir AM. Mutagenic and antimicrobial evaluation of methanol extract from Allium kharputense Freyn Et. Sint 2015; 5(2): 83-7.
• Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006; 19(6-7): 669-75.
• Dziri S, Hassen I, Fatnassi S, Mrabet Y, Casabianca H, Hanchi B, et al. Phenolic constituents, antioxidant and antimicrobial activities of rosy garlic (Allium roseum var. odoratissimum). J Funct Foods. 2012; 4(2): 423-32.
• Figueiredo C, Barroso J, Pedro L, Scheefeer J. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Fragr J. 2007; 22(November): 206-13.
• Amorati R, Valgimigli L. Advantages and limitations of common testing methods for antioxidants. Free Radic Res. 2015; 49(5): 633-49.
• Wollinger A, Perrin E, Chahboun J, Jeannot V, Touraud D, Kunz W. Antioxidant activity of hydro distillation water residues from Rosmarinus officinalis L. leaves determined by DPPH assays. Comptes Rendus Chim. 2016; 19(6): 754-65.
• Feng CY, Wang WW, Ye JF, Li SS, Wu Q, Yin DD, et al. Polyphenol profile and antioxidant activity of the fruit and leaf of Vaccinium glaucoalbum from the Tibetan Himalayas. Food Chem. 2017; 219: 490-5.
• Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006; 19(6-7): 669-75.
• Obanda M, Owuor PO. Flavanol Composition and Caffeine Content of Green Leaf as Qualit y Potential Indicators of Kenyan Black Teas. J Sci Food Agric. 1997; 74: 209-215.
• Tokalıoğlu Ş. Determination of trace elements in commonly consumed medicinal herbs by ICP-MS and multivariate analysis. Food Chem. 2012; 134(4): 2504-8.
• Baye H, Hymete A. Lead and cadmium accumulation in medicinal plants collected from environmentally different sites. Bull Environ Contam Toxicol. 2010; 84(2): 197-201.
• Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V. Multi-element analysis of mineral and trace elements in medicinal herbs and their infusions. Food Chem. 2012; 135(2): 494-501.
• Karak T, Bhagat RM. Trace elements in tea leaves, made tea and tea infusion: A review. Food Res Int. 2010; 43(9): 2234-52.
• Karabegović I, Nikolova M, Veličković D, Stojičević S, Veljković V, Lazić M. Comparison of antioxidant and antimicrobial activities of methanolic extracts of the artemisia sp. recovered by different extraction techniques. Chinese J Chem Eng. 2011; 19(3): 504-11.
• Lim YY, Quah EPL. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem. 2007; 103(3): 734-40.
• Charanjit K, Harish CK, Anti-oxidant activity and total phenolic content of some Asian vegetables, International J Food Sci Technol. 2002; 3: 153-61.
• Grace MH, Esposito D, Dunlap KL, Lila MA. Comparative Analysis of Phenolic Content and Pro fi le, Antioxidant Capacity, and Anti-in flammatory Bioactivity in Wild Alaskan and Commercial Vaccinium Berries. J Agric food Chem. 2014; 62(18): 4007-4017.
• Özcelik B, Lee J, Min D. Effects of Light, Oxygen, and pH on the Absorbance of 2, 2-Diphenyl-1-picrylhydrazyl. J Food Sci. 2003; 68(2): 487-90.
• Wang Y, Gao Y, Ding H, Liu S, Han X, Gui J, et al. Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity. Food Chem. 2017; 218: 152-8.
• Apak R, Güçlü K, Demirata B, Özyürek M, Çelik SE, Bektaşoğlu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007; 12(7): 1496-547.
• Kovats E, Gas-chromatographische Charakterisierung organischer Verbindungen. Teil 1: Retentionsindices aliphatischer Halogenide, Alkohole, Aldehyde und Ketone. Helv Chim Acta. 1958; 41(7): 1915-32.
• Mochizuki E, Yamamoto T, Komiyama Y, Nakazawa H. Identification of Allium Products Using Flame Photometric Detection Gas Chromatography and Distribution Patterns of Volatile Sulfur Compounds. J Agric Food Chem. 1998; 46(12): 5170-6
• Thangam R, Suresh V, Rajkumar M, Vincent JD, Gunasekaran P, Anbazhagan C, et al. Antioxidant and in vitro anticancer effect of 2-pyrrolidinone rich fraction of Brassica oleracea var. capitata through induction of apoptosis in human cancer cells. Phytother Res. 2013; 27(11): 1664-70.
• Terpinc P, Polak T, Šegatin N, Hanzlowsky A, Ulrih NP, Abramovič H. Antioxidant properties of 4-vinyl derivatives of hydroxycinnamic acids. Food Chem. 2011; 128(1): 62-9.
• Bi S-F, Zhu G-Q, Wu J, Li Z-K, Lv Y-Z, Fang L. Chemical composition and antioxidant activities of the essential oil from Nandina domestica fruits. Nat Prod Res. 2016; 30(3): 362-5.
• Carabineiro SA, Bellabarba RM, Gomes PT, Fonseca IM. Aromatisation of 2-phenyl-1-pyrroline to 2-phenylpyrrole using activated carbon. Catal Letters. 2006; 111(3-4): 221-5.
• Da Silva UF, Borba EL, Semir J, Marsaioli AJ. A simple solid injection device for the analyses of Bulbophyllum (Orchidaceae) volatiles. Phytochemistry. 1999; 50(1): 31-4.
• Horton W, Sood A, Peerannawar S, Kugyela N, Kulkarni A, Tulsan R, et al. Synthesis and application of ??-carbolines as novel multi-functional anti-Alzheimer’s disease agents. Bioorganic Med Chem Lett. 2017; 27(2): 232-6.
• Lalitharani S, Mohan VR, Regini GS, Kalidass C. GC-MS analysis of ethanolic extract of Pothos scandens leaf. J Herb Medi Toxicol. 2009; 3(2): 159-60.
• Park SY, Seetharaman R, Ko MJ, Kim DY, Kim TH, Yoon MK, et al. Ethyl linoleate from garlic attenuates lipopolysaccharide-induced pro-inflammatory cytokine production by inducing heme oxygenase-1 in RAW264.7 cells. Int Immunopharmacol. 2014; 19(2): 253-61.
• Jiang Q, Christen S, Shigenaga MK, Ames BN. gamma-Tocopherol, the major form of vitamin E in the US diet, deserves more attention. Am J Clin Nutr. 2001; 74(6): 714-22.
• Karl-Heinz W, Kamal-Eldin A, Elmadfa I. "Gamma-tocopherol-an underestimated vitamin?". Ann nutr metab. 2004; 48(3): 169-188
• Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996; 20(7): 933-56.
• Michael P W. Cadmium carcinogenesis in review. J Inorg Biochem. 2000; 79(1-4): 241-4.
• Cupit M, Larsson O, de Meeûs C, Eduljee GH, Hutton, M, Assessment and management of risks arising from exposure to cadmium in fertilizers-II. Sci Total Environ. 2002; 291; 189-206.