Evaluation of Total Polyphenol Content, Antioxidant Activity and Chemical Composition of Methanolic Extract from Allium Kharputense Freyn et. Sint. and Determination of Mineral and Trace Elements

Year 2017, Volume: 4 Issue: 3, 691 - 708, 01.07.2017

Erdal Yabalak , Ahmet Murat Gizir

https://doi.org/10.18596/jotcsa.315094

Cited By: 11

Abstract

Allium
kharputense Freyn Et. Sint. (A. kharputense) was analyzed for total polyphenol
content, antioxidant activity, chemical composition and mineral and trace
element composition. Total polyphenol content was determined as 257 mg GA 100 g^-1
dried weight by using Folin-Ciocalteu method. DPPH test was performed for antioxidant
activity analysis. IC₅₀ values of gallic acid and Trolox were
determined as 0.02642 mg mL^-1 and 0.225 mg mL^-1,
respectively. IC₅₀ value of A.
kharputense was found as 2.186 mg mL^-1. DPPH free
radical-scavenging activity of extract from 1 mg of A. kharputense was determined 0.01207 mg GAE and 0.1029 mg TrE,
respectively. GC-MS was used to determine chemical composition. Dimethyl
trisulfide, methyl (methylthio)methyl disulfide, 2-Pyrrolidinone,
4-vinylphenol, 2-Phenylpyrrole,
norharmane, linoleic acid, palmitic
acid, γ-Tocopherol and α-Tocopherol are some of 40 compounds which were
detected in methanolic extracts of A.
Kharputense. 20 Mineral and trace elements were determined along with their
levels by ICP-MS using microwave digestion procedure for preparing acid extracts.

Keywords

Antioxidant activity, Allium Kharputense Freyn Et. Sint, Polyphenolic content, DPPH

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