Determination of the isolated Rutin And Quercetin Contents In Syrian Ficus Carica L. Leaves Extracts

Year 2020, Volume: 7 Issue: 1, 197 - 206, 15.02.2020

Shaza Alshaal , Manal Daghestani Francois Karabet

https://doi.org/10.18596/jotcsa.622442

Cited By: 1

Abstract

The
major flavonoid glycoside in Syrian ficus leaves extracts (rutin) was
identified quantitatively and isolated, then transformed into its aglycone
(quercetin) by acidic hydrolysis of ethanolic and aqueous extracts. The
influence of the hydrolysis process on isoquercitrin content was investigated
too. The determination was carried out by high performance liquid
chromatography, and the isolation done by thin layer chromatography plates,
then identified by mass spectrum. Results showed that the rutin content in the
ethanolic extract 78.79% was higher than the aqueous one 61.90%, by using
ultrasonic extraction procedure, the quercetin content by hydrolysis process
was more satisfaction in the ethanolic extract 81.00% than the aqueous one
72.53%. Acidic hydrolysis process destroyed some of the isoquercitrin in the
extracts, the detection of isoquercitrin was done qualitatively by noticing the
mass spectrum and the photo diode array spectrum.  Rutin and quercetin were successfully
isolated, the relative peak areas for isolated rutin and quercetin were 99.80%,
96.46%, respectively. It was concluded that rutin can be easily converted into
quercetin by acidic hydrolysis, allowing the use of Syrian ficus leaves
extracts as an optimal cheap natural antioxidants source.

Keywords

Syrian Ficus Carica L. Leaves, rutin, quercetin

Thanks

Mrs ALyamama Alaskar for her help in acquiring MS spectra.

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