Development of Gelatin-Alginate-TiO2-SOD Biosensor for the Detection of Superoxide Radicals

Year 2020, Volume: 7 Issue: 2, 571 - 580, 23.06.2020

Utku Karakaya Burak Derkuş , Emel Emregul

https://doi.org/10.18596/jotcsa.646433

Cited By: 4

Abstract

In this work, a biosensor that uses
gelatin and alginate hydrogels in addition to titanium dioxide (TiO₂)
nanoparticles (NPs) as sensor matrix was developed in order to detect
superoxide radicals (O2^•-), which play role in carcinogenesis when
present in excess levels. Parameters affecting the performance of the biosensor
such as amount of gelatin-alginate ratio, amount of TiO₂ NPs,
concentration of SOD enzymes and glutaraldehyde cross-linker were investigated.
Electrochemical Impedance Spectroscopy (EIS) and chronoamperometry were used as
electrochemical technique for the development of biosensor as well as
characterisation steps. The developed biosensor exhibited two linear ranges
between 0.0009 mM – 0.125 mM and 0.25 mM – 2 mM which were utilized as
calibration curves. Detection limit of the biosensor was found 0.9 μM, which
was at appropriate level for the detection of O2^•- in tumour
samples. Finally, the constructed biosensor showed significant analytical performance
such as high selection for O2^•-,
low detection limit, and long-term stability.

Keywords

Biyosensör, biosensor, biopolymer, biyopolimer

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