Research Article
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Year 2022, Volume: 9 Issue: 1, 13 - 20, 28.02.2022
https://doi.org/10.18596/jotcsa.945582

Abstract

References

  • 1. Nicolson GL. Metabolic syndrome and mitochondrial function: Molecular replacement and antioxidant supplements to prevent membrane peroxidation and restore mitochondrial function. J Cell Biochem. 2007 Apr 15;100(6):1352–69.
  • 2. Abacı A. Data on prevalence of metabolic syndrome in Turkey: Systematic review, meta-analysis and meta-regression of epidemiological studies on cardiovascular risk factors. Arch Turk Soc Cardiol. 2018; 46(7): 591-601.
  • 3. Vona R, Gambardella L, Cittadini C, Straface E, Pietraforte D. Biomarkers of Oxidative Stress in Metabolic Syndrome and Associated Diseases. Oxidative Medicine and Cellular Longevity. 2019 May 5;2019:1–19.
  • 4. Rochlani Y, Pothineni NV, Kovelamudi S, Mehta JL. Metabolic syndrome: pathophysiology, management, and modulation by natural compounds. Therapeutic Advances in Cardiovascular Disease. 2017 Aug;11(8):215–25.
  • 5. Rytz CL, Pialoux V, Mura M, Martin A, Hogan DB, Hill MD, et al. Impact of aerobic exercise, sex, and metabolic syndrome on markers of oxidative stress: results from the Brain in Motion study. Journal of Applied Physiology. 2020 Apr 1;128(4):748–56.
  • 6. Matés JM, Pérez-Gómez C, De Castro IN. Antioxidant enzymes and human diseases. Clinical Biochemistry. 1999 Nov;32(8):595–603.
  • 7. Townsend DM, Tew KD. The role of glutathione-S-transferase in anti-cancer drug resistance. Oncogene. 2003 Oct;22(47):7369–75.
  • 8. Meng R, Zhu D-L, Bi Y, Yang D-H, Wang Y-P. Anti-oxidative effect of apocynin on insulin resistance in high-fat diet mice. Ann Clin Lab Sci. 2011;41(3):236–43. PMID: 22075506.
  • 9. Zhu C, Schwarz P, Abakumova I, Aguzzi A. Unaltered Prion Pathogenesis in a Mouse Model of High-Fat Diet-Induced Insulin Resistance. Ma J, editor. PLoS ONE. 2015 Dec 14;10(12):e0144983.
  • 10. Mackenzie R, Elliott B. Akt/PKB activation and insulin signaling: a novel insulin signaling pathway in the treatment of type 2 diabetes. DMSO. 2014 Feb;55.
  • 11. Wong SK, Chin K-Y, Suhaimi FH, Fairus A, Ima-Nirwana S. Animal models of metabolic syndrome: a review. Nutr Metab (Lond). 2016 Dec;13(1):65.
  • 12. Kazi TG, Afridi HI, Kazi N, Jamali MK, Arain MB, Jalbani N, et al. Copper, Chromium, Manganese, Iron, Nickel, and Zinc Levels in Biological Samples of Diabetes Mellitus Patients. Biol Trace Elem Res. 2008 Apr;122(1):1–18.
  • 13. Flores CR, Puga MP, Wrobel K, Garay Sevilla MaE, Wrobel K. Trace elements status in diabetes mellitus type 2: Possible role of the interaction between molybdenum and copper in the progress of typical complications. Diabetes Research and Clinical Practice. 2011 Mar;91(3):333–41.
  • 14. Paredes S, Matta-Coelho C, Monteiro A, Fernandes V, Marques O, Alves M. Copper levels, calcium levels and metabolic syndrome. Rev Port Diabetes. 2016;11:99–105.
  • 15. Freitas E, Cunha A, Aquino S, Pedrosa L, Lima S, Lima J, et al. Zinc Status Biomarkers and Cardiometabolic Risk Factors in Metabolic Syndrome: A Case Control Study. Nutrients. 2017 Feb 22;9(2):175.
  • 16. Tükel HC, Alptekin Ö, Turan B, Delilbaşı E. Effects of metabolic syndrome on masseter muscle of male Wistar rats. Eur J Oral Sci. 2015 Dec;123(6):432–8.
  • 17. Noeman SA, Hamooda HE, Baalash AA. Biochemical Study of Oxidative Stress Markers in the Liver, Kidney and Heart of High Fat Diet Induced Obesity in Rats. Diabetol Metab Syndr. 2011 Dec;3(1):17.
  • 18. Ucar F, Sezer S, Erdogan S, Akyol S, Armutcu F, Akyol O. The relationship between oxidative stress and nonalcoholic fatty liver disease: Its effects on the development of nonalcoholic steatohepatitis. Redox Report. 2013 Jul;18(4):127–33.
  • 19. Roncal-Jimenez CA, Lanaspa MA, Rivard CJ, Nakagawa T, Sanchez-Lozada LG, Jalal D, et al. Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake. Metabolism. 2011 Sep;60(9):1259–70.
  • 20. Moreno-Fernández S, Garcés-Rimón M, Vera G, Astier J, Landrier J, Miguel M. High Fat/High Glucose Diet Induces Metabolic Syndrome in an Experimental Rat Model. Nutrients. 2018 Oct 14;10(10):1502.
  • 21. Ghosh S, Sulistyoningrum DC, Glier MB, Verchere CB, Devlin AM. Altered Glutathione Homeostasis in Heart Augments Cardiac Lipotoxicity Associated with Diet-induced Obesity in Mice. Journal of Biological Chemistry. 2011 Dec;286(49):42483–93.
  • 22. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and ?-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985 Jul;28(7):412–9.
  • 23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry. 1951;193:265–75.
  • 24. Buege JA, Aust SD. [30] Microsomal lipid peroxidation. In: Methods in Enzymology [Internet]. Elsevier; 1978 [cited 2021 Nov 20]. p. 302–10.
  • 25. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988 Mar 1;34(3):497–500.
  • 26. Aebi H. [13] Catalase in vitro. In: Methods in Enzymology [Internet]. Elsevier; 1984 [cited 2021 Nov 20]. p. 121–6. Available from:
  • 27. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine. 1967;70(1):158–69.
  • 28. Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. Journal of Biological Chemistry. 1975 Jul;250(14):5475–80.
  • 29. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-Transferases. Journal of Biological Chemistry. 1974 Nov;249(22):7130–9.
  • 30. Mercuri F, Tonutti L, Taboga C, Ceriello A, Assaloni R, Motz E, et al. Detection of nitrotyrosine in the diabetic plasma: evidence of oxidative stress. Diabetologia. 2001 Jul 1;44(7):834–8.
  • 31. Liu H-Y, Hong T, Wen G-B, Han J, Zuo D, Liu Z, et al. Increased basal level of Akt-dependent insulin signaling may be responsible for the development of insulin resistance. American Journal of Physiology-Endocrinology and Metabolism. 2009 Oct;297(4):E898–906.
  • 32. Pereira ENG da S, Silvares RR, Flores EEI, Rodrigues KL, Ramos IP, da Silva IJ, et al. Hepatic microvascular dysfunction and increased advanced glycation end products are components of non-alcoholic fatty liver disease. Gracia-Sancho J, editor. PLoS ONE. 2017 Jun 19;12(6):e0179654.
  • 33. Nestorov J, Glban AM, Mijušković A, Nikolić-Kokić A, Elaković I, Veličković N, et al. Long-term fructose-enriched diet introduced immediately after weaning does not induce oxidative stress in the rat liver. Nutrition Research. 2014 Jul;34(7):646–52.
  • 34. Rubio-Ruiz M, Guarner-Lans V, Cano-Martínez A, Díaz-Díaz E, Manzano-Pech L, Gamas-Magaña A, et al. Resveratrol and quercetin administration improves antioxidant defenses and reduces fatty liver in metabolic syndrome rats. Molecules. 2019 Apr 3;24(7):1297.
  • 35. Jarukamjorn K, Jearapong N, Pimson C, Chatuphonprasert W. A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice. Scientifica. 2016;2016:1–10.
  • 36. Mamun MdAA, Faruk Md, Rahman MdM, Nahar K, Kabir F, Alam MA, et al. High Carbohydrate High Fat Diet Induced Hepatic Steatosis and Dyslipidemia Were Ameliorated by Psidium guajava Leaf Powder Supplementation in Rats. Evidence-Based Complementary and Alternative Medicine. 2019 Feb 3;2019:1–12.
  • 37. Li L, Yang X. The Essential Element Manganese, Oxidative Stress, and Metabolic Diseases: Links and Interactions. Oxidative Medicine and Cellular Longevity. 2018;2018:1–11.
  • 38. Kalita H, Hazarika A, Devi R. Withdrawal of High-Carbohydrate, High-Fat Diet Alters Status of Trace Elements to Ameliorate Metabolic Syndrome in Rats With Type 2 Diabetes Mellitus. Canadian Journal of Diabetes. 2020 Jun;44(4):317-326.e1.

Alterations in Antioxidant Defence Systems and Metal Profiles in Liver of Rats with Metabolic Syndrome Induced with High-Sucrose Diet

Year 2022, Volume: 9 Issue: 1, 13 - 20, 28.02.2022
https://doi.org/10.18596/jotcsa.945582

Abstract

Metabolic syndrome (MetS) is a combination of several different metabolic disorders and considered one of the major public health problems worldwide. The underlying causes of MetS include being overweight and obesity, physical inactivity, and genetic factors. We aimed to examine the alterations in the levels of biomarkers of oxidative stress, activities of antioxidant defense enzymes, and metal contents of the liver in rats with MetS. Rats in control and MetS groups were fed with standard rat chow-drinking water and standard rat chow - 32% sucrose solution (instead of drinking water) ad libitum for 16 weeks, respectively. Following the confirmation of MetS, antioxidant enzyme activities and malondialdehyde (MDA), 3-nitrotyrosine (3-NT), phospho-Akt (pSer473) levels were measured in the homogenates of the liver. Distributions of elements in the liver were also analyzed. The stained hepatic tissue slides were examined by light microscopy. The activities of catalase and glutathione-S-transferase were significantly decreased in MetS-group (about 15% and 29%, respectively) compared to the control group, while the glutathione reductase activity and MDA and 3-NT levels were significantly increased (as the levels of 78%, 26%, and 67%, respectively) (p<0.05). The hepatocytes in the MetS group showed mild diffuse microvesicular steatosis. Furthermore, Cu, Fe, and Mn levels were significantly high in MetS-group while Zn level was significantly low compared to the control group. Our results showed increased oxidative stress, impaired antioxidant defense enzyme activities, and altered metals’ metabolisms which may have an important role in the pathogenesis of MetS.

References

  • 1. Nicolson GL. Metabolic syndrome and mitochondrial function: Molecular replacement and antioxidant supplements to prevent membrane peroxidation and restore mitochondrial function. J Cell Biochem. 2007 Apr 15;100(6):1352–69.
  • 2. Abacı A. Data on prevalence of metabolic syndrome in Turkey: Systematic review, meta-analysis and meta-regression of epidemiological studies on cardiovascular risk factors. Arch Turk Soc Cardiol. 2018; 46(7): 591-601.
  • 3. Vona R, Gambardella L, Cittadini C, Straface E, Pietraforte D. Biomarkers of Oxidative Stress in Metabolic Syndrome and Associated Diseases. Oxidative Medicine and Cellular Longevity. 2019 May 5;2019:1–19.
  • 4. Rochlani Y, Pothineni NV, Kovelamudi S, Mehta JL. Metabolic syndrome: pathophysiology, management, and modulation by natural compounds. Therapeutic Advances in Cardiovascular Disease. 2017 Aug;11(8):215–25.
  • 5. Rytz CL, Pialoux V, Mura M, Martin A, Hogan DB, Hill MD, et al. Impact of aerobic exercise, sex, and metabolic syndrome on markers of oxidative stress: results from the Brain in Motion study. Journal of Applied Physiology. 2020 Apr 1;128(4):748–56.
  • 6. Matés JM, Pérez-Gómez C, De Castro IN. Antioxidant enzymes and human diseases. Clinical Biochemistry. 1999 Nov;32(8):595–603.
  • 7. Townsend DM, Tew KD. The role of glutathione-S-transferase in anti-cancer drug resistance. Oncogene. 2003 Oct;22(47):7369–75.
  • 8. Meng R, Zhu D-L, Bi Y, Yang D-H, Wang Y-P. Anti-oxidative effect of apocynin on insulin resistance in high-fat diet mice. Ann Clin Lab Sci. 2011;41(3):236–43. PMID: 22075506.
  • 9. Zhu C, Schwarz P, Abakumova I, Aguzzi A. Unaltered Prion Pathogenesis in a Mouse Model of High-Fat Diet-Induced Insulin Resistance. Ma J, editor. PLoS ONE. 2015 Dec 14;10(12):e0144983.
  • 10. Mackenzie R, Elliott B. Akt/PKB activation and insulin signaling: a novel insulin signaling pathway in the treatment of type 2 diabetes. DMSO. 2014 Feb;55.
  • 11. Wong SK, Chin K-Y, Suhaimi FH, Fairus A, Ima-Nirwana S. Animal models of metabolic syndrome: a review. Nutr Metab (Lond). 2016 Dec;13(1):65.
  • 12. Kazi TG, Afridi HI, Kazi N, Jamali MK, Arain MB, Jalbani N, et al. Copper, Chromium, Manganese, Iron, Nickel, and Zinc Levels in Biological Samples of Diabetes Mellitus Patients. Biol Trace Elem Res. 2008 Apr;122(1):1–18.
  • 13. Flores CR, Puga MP, Wrobel K, Garay Sevilla MaE, Wrobel K. Trace elements status in diabetes mellitus type 2: Possible role of the interaction between molybdenum and copper in the progress of typical complications. Diabetes Research and Clinical Practice. 2011 Mar;91(3):333–41.
  • 14. Paredes S, Matta-Coelho C, Monteiro A, Fernandes V, Marques O, Alves M. Copper levels, calcium levels and metabolic syndrome. Rev Port Diabetes. 2016;11:99–105.
  • 15. Freitas E, Cunha A, Aquino S, Pedrosa L, Lima S, Lima J, et al. Zinc Status Biomarkers and Cardiometabolic Risk Factors in Metabolic Syndrome: A Case Control Study. Nutrients. 2017 Feb 22;9(2):175.
  • 16. Tükel HC, Alptekin Ö, Turan B, Delilbaşı E. Effects of metabolic syndrome on masseter muscle of male Wistar rats. Eur J Oral Sci. 2015 Dec;123(6):432–8.
  • 17. Noeman SA, Hamooda HE, Baalash AA. Biochemical Study of Oxidative Stress Markers in the Liver, Kidney and Heart of High Fat Diet Induced Obesity in Rats. Diabetol Metab Syndr. 2011 Dec;3(1):17.
  • 18. Ucar F, Sezer S, Erdogan S, Akyol S, Armutcu F, Akyol O. The relationship between oxidative stress and nonalcoholic fatty liver disease: Its effects on the development of nonalcoholic steatohepatitis. Redox Report. 2013 Jul;18(4):127–33.
  • 19. Roncal-Jimenez CA, Lanaspa MA, Rivard CJ, Nakagawa T, Sanchez-Lozada LG, Jalal D, et al. Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake. Metabolism. 2011 Sep;60(9):1259–70.
  • 20. Moreno-Fernández S, Garcés-Rimón M, Vera G, Astier J, Landrier J, Miguel M. High Fat/High Glucose Diet Induces Metabolic Syndrome in an Experimental Rat Model. Nutrients. 2018 Oct 14;10(10):1502.
  • 21. Ghosh S, Sulistyoningrum DC, Glier MB, Verchere CB, Devlin AM. Altered Glutathione Homeostasis in Heart Augments Cardiac Lipotoxicity Associated with Diet-induced Obesity in Mice. Journal of Biological Chemistry. 2011 Dec;286(49):42483–93.
  • 22. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: insulin resistance and ?-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985 Jul;28(7):412–9.
  • 23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry. 1951;193:265–75.
  • 24. Buege JA, Aust SD. [30] Microsomal lipid peroxidation. In: Methods in Enzymology [Internet]. Elsevier; 1978 [cited 2021 Nov 20]. p. 302–10.
  • 25. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988 Mar 1;34(3):497–500.
  • 26. Aebi H. [13] Catalase in vitro. In: Methods in Enzymology [Internet]. Elsevier; 1984 [cited 2021 Nov 20]. p. 121–6. Available from:
  • 27. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. The Journal of laboratory and clinical medicine. 1967;70(1):158–69.
  • 28. Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. Journal of Biological Chemistry. 1975 Jul;250(14):5475–80.
  • 29. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-Transferases. Journal of Biological Chemistry. 1974 Nov;249(22):7130–9.
  • 30. Mercuri F, Tonutti L, Taboga C, Ceriello A, Assaloni R, Motz E, et al. Detection of nitrotyrosine in the diabetic plasma: evidence of oxidative stress. Diabetologia. 2001 Jul 1;44(7):834–8.
  • 31. Liu H-Y, Hong T, Wen G-B, Han J, Zuo D, Liu Z, et al. Increased basal level of Akt-dependent insulin signaling may be responsible for the development of insulin resistance. American Journal of Physiology-Endocrinology and Metabolism. 2009 Oct;297(4):E898–906.
  • 32. Pereira ENG da S, Silvares RR, Flores EEI, Rodrigues KL, Ramos IP, da Silva IJ, et al. Hepatic microvascular dysfunction and increased advanced glycation end products are components of non-alcoholic fatty liver disease. Gracia-Sancho J, editor. PLoS ONE. 2017 Jun 19;12(6):e0179654.
  • 33. Nestorov J, Glban AM, Mijušković A, Nikolić-Kokić A, Elaković I, Veličković N, et al. Long-term fructose-enriched diet introduced immediately after weaning does not induce oxidative stress in the rat liver. Nutrition Research. 2014 Jul;34(7):646–52.
  • 34. Rubio-Ruiz M, Guarner-Lans V, Cano-Martínez A, Díaz-Díaz E, Manzano-Pech L, Gamas-Magaña A, et al. Resveratrol and quercetin administration improves antioxidant defenses and reduces fatty liver in metabolic syndrome rats. Molecules. 2019 Apr 3;24(7):1297.
  • 35. Jarukamjorn K, Jearapong N, Pimson C, Chatuphonprasert W. A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice. Scientifica. 2016;2016:1–10.
  • 36. Mamun MdAA, Faruk Md, Rahman MdM, Nahar K, Kabir F, Alam MA, et al. High Carbohydrate High Fat Diet Induced Hepatic Steatosis and Dyslipidemia Were Ameliorated by Psidium guajava Leaf Powder Supplementation in Rats. Evidence-Based Complementary and Alternative Medicine. 2019 Feb 3;2019:1–12.
  • 37. Li L, Yang X. The Essential Element Manganese, Oxidative Stress, and Metabolic Diseases: Links and Interactions. Oxidative Medicine and Cellular Longevity. 2018;2018:1–11.
  • 38. Kalita H, Hazarika A, Devi R. Withdrawal of High-Carbohydrate, High-Fat Diet Alters Status of Trace Elements to Ameliorate Metabolic Syndrome in Rats With Type 2 Diabetes Mellitus. Canadian Journal of Diabetes. 2020 Jun;44(4):317-326.e1.
There are 38 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Articles
Authors

Özlem Alptekin 0000-0002-0458-7609

Sevde Seyhan Tukel 0000-0003-2503-2781

Belma Turan 0000-0003-2583-9294

Yurdun Kuyucu 0000-0001-6289-0860

Publication Date February 28, 2022
Submission Date September 23, 2021
Acceptance Date November 16, 2021
Published in Issue Year 2022 Volume: 9 Issue: 1

Cite

Vancouver Alptekin Ö, Tukel SS, Turan B, Kuyucu Y. Alterations in Antioxidant Defence Systems and Metal Profiles in Liver of Rats with Metabolic Syndrome Induced with High-Sucrose Diet. JOTCSA. 2022;9(1):13-20.