Exploring the Antibacterial Efficacy of Silver Nanoparticles Synthesized through Abiotic Stress-Induced Germinated Seeds of Vigna radiata: A Comparative Analysis

Year 2024, Volume: 11 Issue: 3, 981 - 994

Tissamol Abraham K. P. Theertha Sachin K. Ashok Jebin Joseph T Sajini

https://doi.org/10.18596/jotcsa.1335103

Abstract

The novel microwave-assisted green synthesis of silver nanoparticles (AgNPs) from stress-induced germinated seeds of Vigna radiata (VR) is explored in this research. AgNPs were successfully synthesized using abiotic stress-induced germinated seeds of VR, induced by salinity, drought, and heavy metals such as sodium chloride (NaCl), polyethylene glycol (PEG), and a chromium solution, respectively. The characterization of the synthesized AgNPs was performed using various techniques, including UV-visible spectrophotometer, dynamic light scattering (DLS), zeta potential, XRD, FT-IR, and FE-SEM. The concentration of AgNPs synthesized from Vr-NaCl, Vr-Cr, Vr-PEG, and Vr-DW followed the order Ag/Vr-DW > Ag/Vr-NaCl > Ag/Vr-PEG > Ag/Vr-Cr. Notably, the synthesized AgNPs exhibited significant antibacterial activity against Staphylococcus aureus bacteria. A comparative analysis of the antibacterial efficacy of AgNPs synthesized using different stress-induced VR seed extracts revealed that AgNPs from PEG stress-induced germinated seeds of VR displayed excellent antibacterial activity. These findings underscore the potential of stress-induced germinated seeds of VR as a promising resource for producing AgNPs with exceptional antibacterial properties, thereby opening avenues for the development of innovative antimicrobial agents.

Keywords

silver nanoparticles, Vigna radiata, microwave, green synthesis, stress-induced germination, antibacterial

Thanks

The authors thank Indian Science Technology and Engineering facilities Map (I-STEM), a Program supported by the Office of the Principal Scientific Adviser to the Govt. of India, for enabling access to the Field emission scanning electron microscopy (FESEM) with Energy Dispersive Spectroscopy (EDS), MAIA3 XMH at the Sophisticated Analytical Instrument Facility (DST-SAIF), Mahatma Gandhi University, Kottayam, India, to carry out this work.

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