Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release

Duygu Alpaslan; Mustafa Yavuzcanli; Abdullah Turan; Tuba Ersen Dudu

doi:10.58692/jotcsb.1682389

Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release

Abstract

Rubia tinctorum L. (Root Dye, Madder) has been historically used in textile dyeing and is valued for its natural antibacterial, antioxidant, and therapeutic properties. In this study, the synthesis of poly(Rubia tinctorum) (p(Rt)) particles from Rubia tinctorum L. extract and their biomedical applications are reported for the first time. The extract and synthesized particles were characterized using SEM, FTIR, DLS, zeta potential analysis, and HPLC. The bioactivity of p(Rt) particles, including antimicrobial, antioxidant, and biocompatibility properties, were assessed. Drug release studies showed significant paracetamol and ceftriaxone release at different pH levels, with release kinetics fitting the Korsmeyer-Peppas model. This study highlights the novelty of p(Rt) particles and their potential in biomedical applications, particularly in drug delivery.

Keywords

Supporting Institution

Van Yuzuncu Yıl University BAP with grant # FYL-2022-10034

Project Number

FYL-2022-10034

Thanks

This work is supported by the Van Yuzuncu Yıl University BAP with grant # FYL-2022-10034

References

Abachi, S., Khademi, F., Fatemi, H., & Malekzadeh, F. (2013). Study of antimicrobial activity of selected Iranian plant extracts on vancomycin resistant Staphylococcus epidermidis. IOSR Journal of Dental and Medical Sciences, 4(1), 59–63.
Alpaslan, D. (2019). Use of colorimetric hydrogel as an indicator for food packaging applications. Bulletin of Materials Science, 42(5), 247. https://doi.org/10.1007/s12034-019-1908-z
Alpaslan, D., Dudu, T. E., & Aktaş, N. (2018). Synthesis, characterization and modification of novel food packaging material from dimethyl acrylamide/gelatin and purple cabbage extract. MANAS Journal of Engineering, 6(2), 110–128.
Alpaslan, D., Dudu, T. E., Şahiner, N., & Aktas, N. (2020). Synthesis and preparation of responsive poly(Dimethyl acrylamide/gelatin and pomegranate extract) as a novel food packaging material. Materials Science and Engineering: C, 108, 110339. https://doi.org/10.1016/j.msec.2019.110339
Alpaslan, D., Ersen Dudu, T., & Aktas, N. (2022a). Agar and Sesame Oil Based Organo-Hydrogels as a Pharmaceutical Excipient in Paracetamol/Carboplatin Release Systems. Indian Journal of Pharmaceutical Sciences, 84(2). https://doi.org/10.36468/pharmaceutical-sciences.929
Alpaslan, D., Ersen Dudu, T., & Aktas, N. (2022b). Evaluation of poly(agar-co-glycerol-co-castor oil) organo-hydrogel as a controlled release system carrier support material. Polymer Bulletin, 79(8), 5901–5922. https://doi.org/10.1007/s00289-021-03777-9
Alpaslan, D., Erşen Dudu, T., & Aktas, N. (2023). Development of onion oil-based organo-hydrogel for drug delivery material. Journal of Dispersion Science and Technology, 44(5), 750–762. https://doi.org/10.1080/01932691.2021.1974869
Alpaslan, D., Olak, T., Turan, A., Ersen Dudu, T., & Aktas, N. (2021). A garlic oil-based organo-hydrogel for use in pH-sensitive drug release. Chemical Papers, 75(11), 5759–5772. https://doi.org/10.1007/s11696-021-01760-2

Alpaslan, D., Olak, T., Turan, A., Ersen Dudu, T., & Aktas, N. (2022). Use of Coconut Oil-Based Organo-Hydrogels in Pharmaceutical Applications. Journal of Polymers and the Environment, 30(2), 666–680. https://doi.org/10.1007/s10924-021-02219-x
Aras Aşci̇, Ö., Demi̇Rci̇, T., & Göktürk Baydar, N. (2018). Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures. International Journal of Secondary Metabolite, 5(3), 210–216. https://doi.org/10.21448/ijsm.453016
Böhm, B. H. H. (1993). Handbuch der Naturfarbstoffe. Lech, Ecomed Verlagsgesellschaft.
Cai, Y., Luo, Q., Sun, M., & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74(17), 2157–2184. https://doi.org/10.1016/j.lfs.2003.09.047
Childs, R. E., & Bardsley, W. G. (1975). The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen. Biochemical Journal, 145(1), 93–103. https://doi.org/10.1042/bj1450093
Derksen, G. C. H., Van Holthoon, F. L., Willemen, H. M., Krul, C. A. M., Franssen, M. C. R., & Van Beek, T. A. (2021). Development of a process for obtaining non-mutagenic madder root (Rubia tinctorum) extract for textile dyeing. Industrial Crops and Products, 164, 113344. https://doi.org/10.1016/j.indcrop.2021.113344
Ersen Dudu, T., Alpaslan, D., & Aktas, N. (2022). Application of Poly (Agar-Co-Glycerol-Co-Sweet Almond Oil) Based Organo-Hydrogels as a Drug Delivery Material. Journal of Polymers and the Environment, 30(2), 483–493. https://doi.org/10.1007/s10924-021-02212-4
Essaidi, I., Snoussi, A., Ben Haj Koubaier, H., Casabianca, H., & Bouzouita, N. (2017). Effect of acid hydrolysis on alizarin content, antioxidant and antimicrobial activities of Rubia tinctorum extracts. Pigment & Resin Technology, 46(5), 379–384. https://doi.org/10.1108/PRT-11-2015-0116
Gilbert, K. G., & Cooke, D. T. (2001). Dyes from plants: Past usage, present understanding and potential. Plant Growth Regulation, 34(1), 57–69. https://doi.org/10.1023/A:1013374618870
Jeremić, S., Filipović, N., Peulić, A., & Marković, Z. (2014). Thermodynamical aspect of radical scavenging activity of alizarin and alizarin red S. Theoretical comparative study. Computational and Theoretical Chemistry, 1047, 15–21. https://doi.org/10.1016/j.comptc.2014.08.007
Korsmeyer, R. W., Gurny, R., Doelker, E., Buri, P., & Peppas, N. A. (1983). Mechanisms of solute release from porous hydrophilic polymers. International Journal of Pharmaceutics, 15(1), 25–35. https://doi.org/10.1016/0378-5173(83)90064-9
Manojlovic, N. T., Solujic, S., Sukdolak, S., & Milosev, M. (2005). Antifungal activity of Rubia tinctorum, Rhamnus frangula and Caloplaca cerina. Fitoterapia, 76(2), 244–246.
Marhoume, F. Z., Aboufatima, R., Zaid, Y., Limami, Y., Duval, R. E., Laadraoui, J., Belbachir, A., Chait, A., & Bagri, A. (2021). Antioxidant and Polyphenol-Rich Ethanolic Extract of Rubia tinctorum L. Prevents Urolithiasis in an Ethylene Glycol Experimental Model in Rats. Molecules, 26(4), 1005. https://doi.org/10.3390/molecules26041005
Marsoul, A., Boukir, A., Ijjaali, M., Taleb, M., Arrousse, N., Salim, R., & Dafali, A. (2023). Phytochemical Characterization, Antioxidant Proprieties and Electrochemical Investigations of Methanolic Extract of Rubia t.L. Roots for LC-Steel Corrosion Protection in 1 M HCl Medium. Journal of Bio- and Tribo-Corrosion, 9(2), 32. https://doi.org/10.1007/s40735-023-00749-6
Mellado, M., Madrid, A., Peña-Cortés, H., López, R., Jara, C., & Espinoza, L. (2013). ANTIOXIDANT ACTIVITY OF ANTHRAQUINONES ISOLATED FROM LEAVES OF MUEHLENBECKIA HASTULATA (J.E. SM.) JOHNST. (POLYGONACEAE). Journal of the Chilean Chemical Society, 58(2), 1767–1770. https://doi.org/10.4067/S0717-97072013000200028
Olak, T., Turan, A., Alpaslan, D., Dudu, T. E., & Aktaş, N. (2020). Developing poly(Agar-co-Glycerol-co-Thyme Oil) based organo-hydrogels for the controlled drug release applications. Journal of Drug Delivery Science and Technology, 60, 102088. https://doi.org/10.1016/j.jddst.2020.102088
Orban, N., Boldizsar, I., Szucs, Z., & Danos, B. (2008). Influence of different elicitors on the synthesis of anthraquinone derivatives in Rubia tinctorum L. cell suspension cultures. Dyes and Pigments, 77(1), 249–257. https://doi.org/10.1016/j.dyepig.2007.03.015
Ozdemir, M. B., & Karadag, R. (2023). Madder ( Rubia tinctorum L.) as an Economic Factor Under Sustainability Goals in the Textile Dyeing. Journal of Natural Fibers, 20(1), 2128968. https://doi.org/10.1080/15440478.2022.2128968
Ruch, R. J., Cheng, S., & Klaunig, J. E. (1989). Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10(6), 1003–1008. https://doi.org/10.1093/carcin/10.6.1003
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3), 144–158. https://doi.org/10.5344/ajev.1965.16.3.144
Siva, R., Palackan, M. G., Maimoon, L., Geetha, T., Bhakta, D., Balamurugan, P., & Rajanarayanan, S. (2011). Evaluation of antibacterial, antifungal, and antioxidant properties of some food dyes. Food Science and Biotechnology, 20(1), 7–13. https://doi.org/10.1007/s10068-011-0002-0
Swain, T. (1966). Comparative phytochemistry. 43, 1791.
Zohra, H. F., Ramazan, E., & Ahmed, H. (2022). Biological activities and chemical composition of rubia tinctorum (l) root and aerial part extracts thereof. Acta Biológica Colombiana, 27(3), 403–414.

Details

Primary Language

English

Subjects

Biomaterial

Journal Section

Research Article

Authors

Duygu Alpaslan ^*
0000-0002-6007-3397
Türkiye

Mustafa Yavuzcanli This is me
0000-0003-0656-1127
Türkiye

Abdullah Turan
0000-0003-3330-4468
Türkiye

Tuba Ersen Dudu This is me
0000-0001-5564-2834
Türkiye

Publication Date

September 15, 2025

Submission Date

April 23, 2025

Acceptance Date

August 21, 2025

Published in Issue

Year 2025 Volume: 8 Number: 2

DOI

https://doi.org/10.58692/jotcsb.1682389

IZ

https://izlik.org/JA27WZ64ST

Cite

RIS / Bibtex

APA

Alpaslan, D., Yavuzcanli, M., Turan, A., & Ersen Dudu, T. (2025). Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release. Journal of the Turkish Chemical Society Section B: Chemical Engineering, 8(2), 185-202. https://doi.org/10.58692/jotcsb.1682389

AMA

1.Alpaslan D, Yavuzcanli M, Turan A, Ersen Dudu T. Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release. JOTCSB. 2025;8(2):185-202. doi:10.58692/jotcsb.1682389

Chicago

Alpaslan, Duygu, Mustafa Yavuzcanli, Abdullah Turan, and Tuba Ersen Dudu. 2025. “Biomedical Potential of P(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release”. Journal of the Turkish Chemical Society Section B: Chemical Engineering 8 (2): 185-202. https://doi.org/10.58692/jotcsb.1682389.

EndNote

Alpaslan D, Yavuzcanli M, Turan A, Ersen Dudu T (September 1, 2025) Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release. Journal of the Turkish Chemical Society Section B: Chemical Engineering 8 2 185–202.

IEEE

[1]D. Alpaslan, M. Yavuzcanli, A. Turan, and T. Ersen Dudu, “Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release”, JOTCSB, vol. 8, no. 2, pp. 185–202, Sept. 2025, doi: 10.58692/jotcsb.1682389.

ISNAD

Alpaslan, Duygu - Yavuzcanli, Mustafa - Turan, Abdullah - Ersen Dudu, Tuba. “Biomedical Potential of P(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release”. Journal of the Turkish Chemical Society Section B: Chemical Engineering 8/2 (September 1, 2025): 185-202. https://doi.org/10.58692/jotcsb.1682389.

JAMA

1.Alpaslan D, Yavuzcanli M, Turan A, Ersen Dudu T. Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release. JOTCSB. 2025;8:185–202.

MLA

Alpaslan, Duygu, et al. “Biomedical Potential of P(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release”. Journal of the Turkish Chemical Society Section B: Chemical Engineering, vol. 8, no. 2, Sept. 2025, pp. 185-02, doi:10.58692/jotcsb.1682389.

Vancouver

1.Duygu Alpaslan, Mustafa Yavuzcanli, Abdullah Turan, Tuba Ersen Dudu. Biomedical Potential of p(Rt) Particles Synthesized from Rubia Tinctorum L. Extract: Characterization, Bioactivity and Controlled Drug Release. JOTCSB. 2025 Sep. 1;8(2):185-202. doi:10.58692/jotcsb.1682389