Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida

Fatma Elif Uzun; Rahime Songür; Emine Bayraktar

doi:10.58692/jotcsb.1880143

Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida

Abstract

Many chemicals used in industry are produced using different isomers of muconic acid. The isomers are especially precursors for many industrially important polymers. In this study, eco-friendly production of cis, cis-muconic acid (ccMA) used in the production of polymers was carried out. Statistical experimental design was applied to optimize the production of ccMA by Pseudomonas putida KT2440 in batch culture. Plackett-Burman design was used to investigate the effect of 11 variables for ccMA production in the first optimization step. The results showed that ccMA production was most influenced by the initial pH of the medium and concentrations of Na-benzoate, NaCl, and (NH4)2SO4. After these significant factors were determined, the optimization of these factors was carried out with Response Surface Methodology (RSM) as the second optimization step. RSM showed that the optimal values of the factors (the initial pH and concentrations of Na-benzoate, NaCl, and (NH4)2SO4) were 7.96, 16.83 mM, 0.7 and 1.4 g/L, respectively. The other components of the fermentation medium were 18 g/L Na2HPO4, 10 g/L KH2PO4, 20 mM glucose, 1 mM MgSO4, 150 µM CaCl2, 13 µM FeSO4, and temperature of 35 oC. ccMA production increased about 4 times using statistical optimization methods.

Keywords

Supporting Institution

The Scientific Research Projects of Ankara University (BAP)

Project Number

17L0443004

Ethical Statement

There is not ethical statement

Thanks

The authors thank to The Scientific Research Projects of Ankara University

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Details

Primary Language

English

Subjects

Chemical Engineering (Other)

Journal Section

Research Article

Authors

Fatma Elif Uzun This is me
0000-0002-1950-4378
Türkiye

Rahime Songür
0000-0002-1511-951X
Türkiye

Emine Bayraktar ^*
0000-0003-1947-3804
Türkiye

Publication Date

March 21, 2026

Submission Date

February 2, 2026

Acceptance Date

February 23, 2026

Published in Issue

Year 2026 Volume: 2026

DOI

https://doi.org/10.58692/jotcsb.1880143

IZ

https://izlik.org/JA86RT72WR

Cite

RIS / Bibtex

APA

Uzun, F. E., Songür, R., & Bayraktar, E. (2026). Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida. Journal of the Turkish Chemical Society Section B: Chemical Engineering, 2026, 1-14. https://doi.org/10.58692/jotcsb.1880143

AMA

1.Uzun FE, Songür R, Bayraktar E. Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida. JOTCSB. 2026;2026:1-14. doi:10.58692/jotcsb.1880143

Chicago

Uzun, Fatma Elif, Rahime Songür, and Emine Bayraktar. 2026. “Statistical Optimization of Fermentation Conditions for Sustainable Cis,cis-Muconic Acid Biosynthesis by Pseudomonas Putida”. Journal of the Turkish Chemical Society Section B: Chemical Engineering 2026 (March): 1-14. https://doi.org/10.58692/jotcsb.1880143.

EndNote

Uzun FE, Songür R, Bayraktar E (March 1, 2026) Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida. Journal of the Turkish Chemical Society Section B: Chemical Engineering 2026 1–14.

IEEE

[1]F. E. Uzun, R. Songür, and E. Bayraktar, “Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida”, JOTCSB, vol. 2026, pp. 1–14, Mar. 2026, doi: 10.58692/jotcsb.1880143.

ISNAD

Uzun, Fatma Elif - Songür, Rahime - Bayraktar, Emine. “Statistical Optimization of Fermentation Conditions for Sustainable Cis,cis-Muconic Acid Biosynthesis by Pseudomonas Putida”. Journal of the Turkish Chemical Society Section B: Chemical Engineering 2026 (March 1, 2026): 1-14. https://doi.org/10.58692/jotcsb.1880143.

JAMA

1.Uzun FE, Songür R, Bayraktar E. Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida. JOTCSB. 2026;2026:1–14.

MLA

Uzun, Fatma Elif, et al. “Statistical Optimization of Fermentation Conditions for Sustainable Cis,cis-Muconic Acid Biosynthesis by Pseudomonas Putida”. Journal of the Turkish Chemical Society Section B: Chemical Engineering, vol. 2026, Mar. 2026, pp. 1-14, doi:10.58692/jotcsb.1880143.

Vancouver

1.Fatma Elif Uzun, Rahime Songür, Emine Bayraktar. Statistical Optimization of Fermentation Conditions for Sustainable cis,cis-Muconic Acid Biosynthesis by Pseudomonas putida. JOTCSB. 2026 Mar. 1;2026:1-14. doi:10.58692/jotcsb.1880143