EGGSHELL DERIVED NANOHYDROXYAPATITE REINFORCED CHITOSAN CRYOGEL BIOCOMPOSITES FOR TISSUE ENGINEERING APPLICATIONS

Abstract

Hydroxyapatite has a biocompatible, biodegradable and natural apatite characteristic to be used in biomedical applications such as bone tissue engineering. The objectives of this study were to synthesize hydroxyapatite from domestic waste eggshells which is utilized as pure calcium source; compare the properties of biosynthesized hydroxyapatite with commercially purchased hydroxyapatite; and produce biosynthesized hydroxyapatite reinforced chitosan cryogels for possible tissue engineering applications. Calcium oxide powders obtained after calcination of waste eggshells showed different particle sizes depending on calcination temperature. It was found that increased temperature of calcination led to the powders of smaller particle sizes. Structural changes at carbonate groups of calcined eggshell were determined by FTIR analysis. The effect of the biosynthesized hydroxyapatite on the morphology of chitosan cryogel biocomposites were determined. The changes in the chemical bond structure of the cryogels were analyzed by FTIR and swelling behavior of produced chitosan cryogels was determined by swelling ratio tests.

Keywords

• Biosynthesis,eggshell,hydroxyapatite,chitosan,biocomposite scaffold

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