Detection of MDA titer of ınfectious bronchitis virus and comparison of antibody titer produced by two different ınfectious bronchitis vaccine

Abstract

The infectious bronchitis (IB) is divesting infectious diseases of poultry. Hence, this study determined the maternally derived antibody (MDA) titer of IBV and comparison of two IB vaccine in layer. A total of 1600 birds were equally allocated into two groups (group-A and group-B). The group-A and group-B were vaccinated by two different vaccines having the strain “B1-Strain of ND+Massachusetts B-41 Strains of IBV” and “Hitchner-B1 Strain of ND+Massachusetts H120 Strains of IBV” respectively. Blood samples were collected and antibody titer was detected by indirect ELISA test. Results showed that both groups had the protective level of MDA titer and both the vaccines significantly (p<0.01) increased the antibody mean titer than the MDA. Between the groups, the highest antibody mean titer (11544.43±177.51) was observed in group-B at 5 weeks age and had significant (p<0.01) difference with group-A (10222.11±96.96). The effect size of the antibody titer was higher in group-B (14.25; CI: 9173.82-9564.39; n=46 at 5 weeks birds) than group-A (9.54; CI: 8580.19-9131.68; n=46 at one-day-old birds) respectively. Shortly, the MDA mean titer was in protective condition and both the IB vaccinated groups had the significantly (p<0.05) higher antibody mean titer than the protective level of antibody titer (>853).

Keywords

• Antibody Titer
• Indirect ELISA
• MDA
• Vaccine