Different concentrations of high fructose corn syrup in broiler diet cause different effects on selected hematological parameters

Year 2024, , 22 - 26, 30.06.2024

Taha Altuğ , Gökhan Şen , Ruhi Kabakçı

https://doi.org/10.34233/jpr.1507530

Abstract

This study was aimed to investigate how the consumption of high fructose corn syrup (HFCS) affects blood parameters of broilers. Total 120 chickens were divided into three groups including 4 subgroups in each with free access to food and water for 42 days. The control group received no additional treatment, while the second and third groups were fed diets containing 5% or 10% HFCS, respectively. On the last day, broilers were euthanized, and 5 mL blood samples were collected for hematological analyses. Results indicated no significant differences in red blood cell (RBC) or hemoglobin (HGB) levels between the control and 5% or 10% HFCS- treated groups. However, 10% HFCS treatment significantly increased packed cell volume (PCV) and mean corpuscular volume (MCV), while 5%-HFCS increased mean corpuscular hemoglobin (MCH) and decreased mean corpuscular hemoglobin concentration (MCHC) compared to the control group. Total leukocyte counts (TLC) and monocyte values remained unaffected by HFCS treatments. Notably, 5% HFCS treatment increased basophil, heterophil, and
heterophil/lymphocyte ratio while decreasing lymphocyte. Conversely, 10% HFCS treatment reduced eosinophil and heterophil, increasing lymphocyte counts. In conclusion, our study suggests that dietary fructose intake can modify certain hematological parameters, potentially serving as early indicators of future systemic or metabolic issues.

Keywords

HFSC, Poultry, Blood parameters, Heterophil to lymphocyte ratio Stress

Ethical Statement

The scientific design of this study was approved by the Local Ethical Committee of Kırıkkale University with the decision date and number of 2020-06/06.

Supporting Institution

No commercial or financial fund have been obtained to support this study.

References

• Abd Elmonem, H. and Ali, E. 2011. Risks of a fructose rich soft drink consumption on some biochemical parameters in Balb/c mice. Journal of Radiation Research and Applied Sciences. , 4: 1373-1388.
• Akhlaghi, A., Ahangari, Y. J., Zhandi, M. and Peebles, E. 2014. Reproductive performance, semen quality, and fatty acid profile of spermatozoa in senescent broiler breeder roosters as enhanced by the long-term feeding of dried apple pomace. Animal Reproduction Science. , 147(1-2): 64-73.
• Alberts B, J. A., Lewis J, et al. 2002. How Cells Obtain Energy from Food. Molecular Biology of the Cell. New York, Garland Science: 47-129.
• Arnone, D., Chabot, C., Heba, A.-C., Kökten, T., Caron, B., Hansmannel, F., Dreumont, N., Ananthakrishnan, A. N., Quilliot, D. and Peyrin-Biroulet, L. 2022. Sugars and gastrointestinal health. Clinical Gastroenterology and Hepatology, 20(9): 1912-1924. e1917.
• Aviagen 2014. Ross 308 broiler breeder’s handbook. http://tr.aviagen.com/assets/Tech_Center/BB_Foreign_Language_Docs/TR_TechDocs/RossBroilerHandbook2014-TR.pdf, Aviagen.
• Bakalov, D., Sabit, Z., Tafradjiiska, R. and Andreeva-Gateva, P. 2021. Effects of antioxidant diet on the RBC indices in fructose-induced obesity rat model. Acta Natura et Scientia, 8(3): 21-28.
• Campbell, T. W. and Ellis, C. K. 2007. Avian and exotic animal hematology and cytology. Ames-Iowa-USA, John Wiley & Sons. Ebrahimi, A., Qotbi, A. A. A., Seidavi, A., Laudadio, V. and Tufarelli, V. 2013. Effect of different levels of dried sweet orange (Citrus sinensis) peel on broiler chickens growth performance Abbas. Archives Animal Breeding, 56(1): 11-17.
• Fedewa, A. and Rao, S. S. 2014. Dietary fructose intolerance, fructan intolerance and FODMAPs. Current gastroenterology reports, 16(1): 1-8.
• Galderisi, A., Giannini, C., Van Name, M. and Caprio, S. 2019. Fructose Consumption Contributes to Hyperinsulinemia in Adolescents With Obesity Through a GLP-1–Mediated Mechanism. The Journal of Clinical Endocrinology and Metabolism, 104(8): 3481-3490.
• Goñí, I., Brenes, A., Centeno, C., Viveros, A., Saura-Calixto, F., Rebolé, A., Arija, I. and Estevez, R. 2007. Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility, and susceptibility to meat lipid oxidation in chickens. Poultry Science, 86(3): 508-516.
• Gross, W. B., and H. S. Siegel. 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Diseases, 27(4): 972-979.
• Jung, C.-G., Yang, E.-M., Lee, J.-H., Kim, S.-H., Park, H.-S. and Shin, Y. S. 2018. Coca-Cola allergy identified as fructose-induced anaphylaxis. The Journal of Allergy and Clinical Immunology, 6(5): 1787-1789.
• Kabakci, R. and Kara, E. 2023. The Alterations of Erythrocyte Osmotic Fragility and Hematological Parameters in Puppies with Parvoviral Enteritis in Kirikkale, Türkiye. International Journal of Veterinary and Animal Research (IJVAR), 6(2): 66-71.
• Kabakci, R., Yigit, AA., Kara, E., Cinar, M., Ekici, H., Aluc Y. 2022. Age-and sex-related changes in selected hematological parameters, lipid peroxidation and erythrocytes osmotic fragility of Turkish Angora cats. The Journal of the Hellenic Veterinary Medical Society, 73(1): 3715-3722.
• Mihafu, F. D., Kiage, B. N., Kimang’a, A. N. and Okoth, J. K. 2020. Effect of chia seeds (Salvia hispanica) on postprandial glycaemia, body weight and hematological parameters in rats fed a high fat and fructose diet. International Journal of Biological and Chemical Sciences 14(5): 1752-1762.
• Moughaizel, M., Dagher, E., Jablaoui, A., Thorin, C., Rhimi, M., Desfontis, J.-C. and Mallem, Y. 2022. Long-term high-fructose high-fat diet feeding elicits insulin resistance, exacerbates dyslipidemia and induces gut microbiota dysbiosis in WHHL rabbits. Plos one, 17(2): e0264215.
• NRC 1994. Nutrient Requirements of Poultry. Washington DC., USA, National Research Council.
• Nurliyani, N., Harmayani, E. and Sunarti, S. 2018. Goat milk kefir supplemented with porang glucomannan improves lipid profile and haematological parameter in rat fed high fat and high fructose diet. Romanian Journal of Diabetes Nutrition and Metabolic Diseases, 25(1): 11-21.
• Okon, I. A., Ufot, U. F., Onoyeraye, U. G., Nwachukwu, E. O. and Owu, D. U. 2019. Effect of Gongronema latifolium on lipid profile, oral glucose tolerance test and some hematological parameters in fructose induced hyperglycemia in rats. Pharmaceutical and Biomedical Research, 5(1): 25-27.
• Ouchi, Y., Komaki, Y., Shimizu, K., Fukano, N., Sugino, T., Shiraishi, J. I., ... & Bungo, T. (2023). Comparison of oral administration of fructose and glucose on food intake and physiological parameters in broiler chicks. Poultry science, 102(1), 102249.
• Parker, K., Salas, M. and Nwosu, V. C. 2010. High fructose corn syrup: production, uses and public health concerns. Biotechnology and Molecular Biology Reviews, 5(5): 71-78.
• Rippe, J. M. and Angelopoulos, T. J. 2013. Sucrose, high-fructose corn syrup, and fructose, their metabolism and potential health effects: what do we really know? Advances in nutrition, 4(2): 236-245.
• Scanes, C. G. 2016. Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio. Poultry science, 95(9): 2208-2215.
• Skoog, S. M. and Bharucha, A. E. 2004. Dietary fructose and gastrointestinal symptoms: a review. The American Journal of Gastroenterology, 99(10): 2046-2050.
• Stewart, A. S., Pratt-Phillips, S. and Gonzalez, L. M. 2017. Alterations in intestinal permeability: the role of the “leaky gut” in health and disease. Journal of equine veterinary science, 52: 10-22.
• Stonestreet, B. S., Goldstein, M., Oh, W. and Widness, J. A. 1989. Effects of prolonged hyperinsulinemia on erythropoiesis in fetal sheep. The American journal of physiology, 257(5): 1199-1204.
• Şen, G. and Başalan, M. 2017. Selülozun Rumende Parçalanabilirliğini Geliştirme Stratejileri. Turkiye Klinikleri Animal Nutrition and Nutritional Diseases - Special Topics 3(3): 190-198.
• Tamzil, M., Haryani, N. and Indarsih, B. 2016. Reduced Expression of Heat Shock Protein (HSP) 70 Gene by ascorbic acid supplementation in broiler chickens exposed to transportation stress to maintain the quality of meat and hematological parameters. International Journal of Poultry Science, 15(11): 432-441.
• Theml, H., Diem, H. and Haferlach, T. 2004. Color atlas of hematology: practical microscopic and clinical diagnosis. Stuttgart - New York, Thieme. Yu, R., Yang, B., Cai, L., Lu, X. and Wang, X. 2018. Excess free fructose beverages and allergy in children and adolescents: Results from NHANES 2005-2006. Annals of Family Medicine, 16(5): 408-418.