Bir Hindi Sürüsünde Eş Zamanlı Koligranülomatozis ve Marek Hastalığı Üzerine Patolojik ve İmmunohistokimyasal İncelemeler

Year 2020, Volume: 17 Issue: 2, 87 - 95, 30.12.2020

Mehmet Burak Ateş , Zeynep Çelik , Mustafa Çiftçi

https://doi.org/10.34233/jpr.744360

Abstract

Kanatlılarda hepatik granülomlara neden olan Koligranülomatozis ve önemli neoplastik hastalıklarından biri olan Marek, hindilerde nadiren salgınlara neden olan hastalıklardır. Bu çalışmada Konya bölgesinde ticari bir hindi işletmesinde ölen hindilerin post-mortem, histopatolojik, mikrobiyolojik ve immunohistokimyasal incelemeleri ışığında etiyolojik tanıları yapılmıştır. Post-mortem muayenede karaciğerde parankime yayılan çok sayıda 3-10 mm çapında gri-beyaz renkli odaklara rastlanıldı. Bağırsakların tüm sekmenlerinde mukuslu eksudat ve hiperemi belirlendi. Mikroskobik muayene için tüm dokular Hematoksilen-Eozin, seçilen bazı karaciğer dokuları ise Periodic acid-Schiff, Ziehl-Neelsen ve Giemsa ile boyandı. Karaciğerde, ortalarında eizonofilik bir nekrotik kitle bulunan tipik piyogranülom yapıları belirlendi. Ayrıca karaciğer, dalak ve bağırsaklarda (jejenum ve sekum) pleomorfik histiyosit ve lenfoid hücrelerin oluşturduğu tümör odakları gözlendi. Seçilen karaciğer ve bağırsak kesitlerine Marek hastalığı virüsüne spesifik primer antikorla yapılan immunohistokimyasal boyamalarda tümör hücrelerinde pozitif reaksiyon elde edildi. Ayrıca karaciğerde lezyonlu bölgelerden alınan swap ve örneklerde mikrobiyolojik ekim sonucu Escherichia coli izolasyonu gerçekleştirildi. Yapılan incelemeler sonucunda, hindilerde hepatik granülomlara neden olabilecek diğer etiyolojik ajanlar da dışlanarak, bu hindi sürüsünde eş zamanlı olarak seyreden koligranülomatozis ve Marek hastalığı teşhisi konuldu. Bu hastalıkların birbiri ile etkileşim gösterebileceği ve iki hastalığın aynı anda gözlendiği olgularda ölüm vakalarının artabileceği sonucuna varıldı.

Keywords

Koligranüloma (Hjarre’s hastalığı), Histopatoloji, İmmunohistokimya, Marek hastalığı, Hindi

Pathological and Immunohistochemical Examinations on Co-Infection with Coligranulomatosis and Marek's Disease in a Turkey Flock

Abstract

Coligranulomatosis, which induces hepatic granulomas, and Marek's disease, one of the important neoplastic diseases in poultry, rarely causes outbreaks in turkeys. In this study, etiological diagnoses were performed in the light of post-mortem, histopathological, microbiological, and immunohistochemistry studies of turkeys that died in a commercial establishment in the region of Konya. In the post-mortem examination, many grey-white foci with a diameter of 3-10 mm, which sometimes spread from serosa to the parenchyma, were found in the liver. Mucous exudate and hyperemia were detected in all segments of the intestine. For microscopic examination, all tissues were stained with Haematoxylin-Eosin and some selected liver tissues with Periodic Acid-Schiff, Ziehl-Neelsen, and Giemsa. Typical structures of pyogranulomas with an eosinophilic necrotic core in the liver were identified. Tumor foci formed by pleomorphic histiocyte and lymphoid cells were also observed in the liver, spleen, and intestines (jejunum and cecum). In the selected liver and intestinal sections, a positive reaction was obtained in tumor cells in immunohistochemical staining with the primary antibody specific for Marek's disease virus. In addition, Escherichia coli isolation was performed microbiologically in swaps and samples taken from lesioned areas of the liver. As a result of the examinations, other etiological agents that may cause hepatic granulomas in the turkeys were excluded, and coligranulomatosis and Marek's disease were diagnosed simultaneously in this turkey flock. It was concluded that in cases in which these two diseases are observed simultaneously, the diseases might interact with each other, and casualties may increase.

Keywords

Coligranuloma (Hjarre’s disease), histopathology, immunohistochemistry, Marek’s disease, turkey

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