Research Article
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Year 2024, Volume: 28 Issue: 6, 1883 - 1891, 28.06.2025
https://doi.org/10.29228/jrp.861
https://izlik.org/JA56SE53AX

Abstract

References

  • [1] Ali ZS, Khudair KK. Synthesis, characterization of silver nanoparticles using Nigella sativa seeds and study their effects on the serum lipid profile and DNA damage on the rats’ blood treated with Hydrogen peroxide. The Iraqi Journal of Veterinary Medicine. 2019;43(2):23-37. https://doi.org/10.30539/iraqijvm.v43i2.526
  • [2] Shaker AF, Ibrahim OM. Gold nanoparticle-vancomycin delivery: synthesis and antibacterial evaluation against methicillin-resistant staphylococcus aureus. Biochemical & Cellular Archives. 2022; 1;22(1): 1793-1800. https://connectjournals.com/03896.2022.22.1793
  • [3] Damiani G, Eggenhöffner R, Pigatto PDM, Bragazzi NL. Nanotechnology meets atopic dermatitis: Current solutions, challenges and future prospects. Insights and implications from a systematic review of the literature. Bioact Mater. 2019;4:380-386. https://doi.org/10.1016/j.bioactmat.2019.11.003
  • [4] Saliem AH, Ibrahim OM, Salih SI. Biosynthesis of silver nanoparticles using Cinnamon zeylanicum plants bark extract. Kufa journal for veterinary medical sciences, 2016;7(1): 51-63
  • [5] Botteon CEA, Silva LB, Ccana-Ccapatinta GV, Silva TS, Ambrosio SR, Veneziani RCS, Bastos JK, Marcato PD. Biosynthesis and characterization of gold nanoparticles using Brazilian red propolis and evaluation of its antimicrobial and anticancer activities. Sci Rep. 2021;11(1):1974. https://doi.org/10.1038/s41598-021-81281-w
  • [6] Abdulsattar SA. Biological impact of gold nanoparticle on estradaiol and testosterone levels in sera of human males. Journal of the Faculty of Medicine Baghdad. 2015;57(4):333-7. https://doi.org/10.32007/jfacmedbagdad.574403
  • [7] Al-Shaabani MJM, Al-Ethawi AMT, Al-Mathkhury HJF. Eco-friendly synthesis of gold nanoparticles and study their effect with antibiotics against Acinetobacter baumannii. Iraqi Journal of Agricultural Sciences 2020;51(4): 1204-1211. https://doi.org/10.36103/ijas.v51i4.1099
  • [8] Ayad ZM, Ibrahim OMS, Omar LW. Biosynthesis and characterization of silver nanoparticles by Silybum marianum (silymarin) fruit extract. Adv. Anim. Vet. Sci. 2019; 7(2):122-130. http://dx.doi.org/10.17582/journal.aavs/2019/7.2.122.130
  • [9] Arslan, K. (2023). The Antioxidant, Antimicrobial, and Total Phenolic Potential of Clove Extracts for Inhibition of Food Pathogens. Erzincan University Journal of Science and Technology, 16(2), 453-464. https://doi.org/10.18185/erzifbed.1279953
  • [10] Ayoola GA, Lawore FM, Adelowotan T, Aibinu IE, Adenipekun E, Coker HAB, Odugbemi, TO. Chemical analysis and antimicrobial activity of the essential oil of Syzigium aromaticum (clove). Afr J Microbiol Res, 2008;2(7): 162-166.
  • [11] Ali BM, Ibrahim O. Antifungal Activity of Clove (Syzygium aromaticum) Essential Oil Extract against Induced Topical Skin Infection by Candida albicans in Mice in vivo. The Egyptian Journal of Hospital Medicine. 2023;91(1):3855-61. https://dx.doi.org/10.21608/ejhm.2023.293468
  • [12] Kapadiya S, Desai MA. Isolation of essential oil from buds of Syzygium aromaticum using hydrodistillation: multi response optimization and predictive modeling, International Journal of Advance Research in Science and Engineering. 2017;6 (1).
  • [13] Bhuiyan MNI, Begum J, Nandi NC, Akter F. Constituents of the essential oil from leaves and buds of clove (Syzigium caryophyllatum (L.) Alston). African Journal of Plant Science, 2010;4(11): 451-454.
  • [14] Razafimamonjison G, Jahiel M, Duclos T, Ramanoelina P, Fawbush F, Danthu P. Bud, leaf, and stem essential oil composition of Syzygium aromaticum from Madagascar, Indonesia, and Zanzibar. International Journal of Basic and Applied Sciences, 2014;3(3): 224-233. http://dx.doi.org/10.14419/ijbas.v3i3.2473
  • [15] Takahashi H, Nakamura A, Fujino N, Sawaguchi Y, Sato M, Kuda T, Kimura B. Evaluation of the antibacterial activity of allyl isothiocyanate, clove oil, eugenol and carvacrol against spoilage lactic acid bacteria. LWT. 2021;145:111263.https://doi.org/10.1016/j.lwt.2021.111263
  • [16] Zari AT, Zari TA, Hakeem KR. Anticancer Properties of Eugenol: A Review. Molecules. 2021;26(23):7407. https://doi.org/10.3390/molecules26237407
  • [17] Safrudin I, Maimulyanti A, Prihadi AR. Effect of crushing of clove bud (Syzygium aromaticum) and distillation rate on main constituents of the essential oil. American Journal of Essential Oils and Natural Products. 2015;2(3):12-5.
  • [18] Gaspar EM, Duarte, R, & Santana JC. Volatile composition and antioxidant properties of clove products. Biomedical Journal of Scientific and Technical Research, 2018; 9(4), 7270-7276. http://dx.doi.org/10.26717/BJSTR.2018.09.001831
  • [19] Lee S, Najiah M, Wendy W, Nadirah M. Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites. Frontiers of Agriculture in China, 2009;3: 332-336. http://dx.doi.org/10.1007/s11703-009-0052-8
  • [20] Selles SMA, Kouidri M, Belhamiti BT, Ait Amrane A. Chemical composition, in-vitro antibacterial and antioxidant activities of Syzygium aromaticum essential oil. Food Measure. 2020;14(4):2352–8. https://doi.org/10.1007%2Fs11694 020-00482-5
  • [21] Nee Kamaldeep KS, Kaur S, Bhalla V, Kumar M, Gupta A. Pentacenequinone derivatives for the preparation of gold nanoparticles: facile synthesis and catalytic application. Journal of Materials Chemistry A, 2014;2(22): 8369-8375. https://doi.org/10.1039/C4TA00397G
  • [22] Basavaraja S, Balaji SD, Lagashetty A, Rajasab AH, Venkataraman A. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Materials Research Bulletin, 2008;43(5): 1164-1170. http://dx.doi.org/10.1016/j.materresbull.2007.06.020
  • [23] Carone A, Emilsson S, Mariani P, Désert A, Parola S. Gold nanoparticle shape dependence of colloidal stability domains. Nanoscale Adv. 2023;5(7):2017-2026. https://doi.org/10.1039%2Fd2na00809b
  • [24] Ebrahimzadeh MA, Naghizadeh A, Mohammadi-Aghdam S, Khojasteh H, Ghoreishi SM, Mortazavi-Derazkola S. Enhanced catalytic and antibacterial efficiency of biosynthesized Convolvulus fruticosus extract capped gold nanoparticles (CFE@AuNPs). J Photochem Photobiol B. 2020;209:111949. https://doi.org/10.1016/j.jphotobiol.2020.111949
  • [25] Siddique S, Chow JC. Gold nanoparticles for drug delivery and cancer therapy. Applied Sciences, 2020;10(11): 3824. https://doi.org/10.3390/app10113824
  • [26] Vilas V, Philip D, Mathew J. Biosynthesis of Au and Au/Ag alloy nanoparticles using Coleus aromaticus essential oil and evaluation of their catalytic, antibacterial and antiradical activities. Journal of Molecular Liquids,2016; 221: 179-189. http://dx.doi.org/10.1016/j.molliq.2016.05.066
  • [27] Meena Kumari M, Philip D. Facile one-pot synthesis of gold and silver nanocatalysts using edible coconut oil. Spectrochim Acta A Mol Biomol Spectrosc. 2013;111:154-60. https://doi.org/10.1016/j.saa.2013.03.076
  • [28] Do PQT, Huong VT, Phuong NTT, Nguyen TH, Ta HKT, Ju H, Phan TB, Phung VD, Trinh KTL, Tran NHT. The highly sensitive determination of serotonin by using gold nanoparticles (Au NPs) with a localized surface plasmon resonance (LSPR) absorption wavelength in the visible region. RSC Adv. 2020;10(51):30858-30869. https://doi.org/10.1039%2Fd0ra05271j
  • [29] Husain WM, Araak JK, Ibrahim OMS. Effect of different doses from znonps on the pituitary-testes axis function in adult male rats.2019. Adv. Anim. Vet. Sci, 7(7), 550-556. http://dx.doi.org/10.17582/journal.aavs/2019/7.7.550.556
  • [30] Hussein SI, Shubber SS, Yaseen NY. Bio-distribution of Gold Nanoparticles in Tumor Mass and Different Organs in Implanted Mice with Mammary Adenocarcinoma AM3 (in vivo study). The Iraqi Journal of Veterinary Medicine. 2019 Dec 28;43(2):17-22. https://doi.org/10.30539/iraqijvm.v43i2.525.
  • [31] Pramod SK, Navnath, KA, Pramod SM. A revıew on gas chromatography-mass spectrometry (GC-MS). World J. Pharm. Res. 2021; 10(3): 741-763.https://doi.org/10.17605/OSF.IO/GAFMT
  • [32] Yassin MT, Mostafa AAF, Al-Askar AA. In vitro, anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis. BMC complementary medicine and therapies, 2020;20(1): 1-9. https://doi.org/10.1186/s12906-020-2818-8
  • [33] Singh AK, Talat M, Singh DP, Srivastava ON. Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with an amine group. Journal of Nanoparticle Research, 2010; 12, 1667-1675. http://dx.doi.org/10.1007/s11051-009-9835-3
  • [34] Azam A, Ahmed F, Arshi N, Chaman M, Naqvi AH. One-step synthesis and characterization of gold nanoparticles and their antibacterial activities against E. coli (ATCC 25922 strain). Int J Theor Appl Sci, 2009;1(2): 1-4.
  • [35] Rasmussen MK, Pedersen JN, Marie R. Size and surface charge characterization of nanoparticles with a salt gradient. Nat Commun. 2020;11(1):2337. https://doi.org/10.1038/s41467-020-15889-3
  • [36] Ghosh P, Han G, De M, Kim CK, Rotello VM. Gold nanoparticles in delivery applications. Adv Drug Deliv Rev. 2008;60(11):1307-1315. https://doi.org/10.1016/j.addr.2008.03.016
  • [37] Bennur T, Khan Z, Kshirsagar R, Javdekar V, Zinjarde S. Biogenic gold nanoparticles from the Actinomycete Gordonia amarae: application in rapid sensing of copper ions. Sensors and Actuators B: Chemical, 2016;233: 684-690. http://dx.doi.org/10.1016/j.snb.2016.04.022
  • [38] Ankamwar B. Biosynthesis of gold nanoparticles (green-gold) using leaf extract of Terminalia catappa. E-Journal of Chemistry, 2010;7(4):1334-1339. https://doi.org/10.1155/2010/745120

Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation

Year 2024, Volume: 28 Issue: 6, 1883 - 1891, 28.06.2025
https://doi.org/10.29228/jrp.861
https://izlik.org/JA56SE53AX

Abstract

For the synthesis of metal nanoparticles, essential oils have the potential to serve as stabilizers and reducing agents at the same time. The aim of this study was to prepare and characterize gold nanoparticles (AuNPs) by green synthesis from clove oil. The clove oil (Syzygium aromaticum) was used in the current research program to create affordable and environmentally friendly gold nanoparticles. Ultraviolet -visible, X-ray diffraction (XRD) , zeta potential, particle size, and scanning electron microscope (SEM) methods were used to characterize the nanoparticles. It was discovered that the UV-visible absorption peak was 562 nm, demonstrating the polydispersity of the nanoparticles. The XRD data indicated that the gold nanoparticles' surfaces were crystalline in nature. The zeta potential obtained was 90.26 mV, which indicates that the nanoparticles were stable and not aggregated. SEM indicates the regular spherical shape of the nanoparticles. Particle size effects were thought to be the cause of the peaks in the XRD patterns.

References

  • [1] Ali ZS, Khudair KK. Synthesis, characterization of silver nanoparticles using Nigella sativa seeds and study their effects on the serum lipid profile and DNA damage on the rats’ blood treated with Hydrogen peroxide. The Iraqi Journal of Veterinary Medicine. 2019;43(2):23-37. https://doi.org/10.30539/iraqijvm.v43i2.526
  • [2] Shaker AF, Ibrahim OM. Gold nanoparticle-vancomycin delivery: synthesis and antibacterial evaluation against methicillin-resistant staphylococcus aureus. Biochemical & Cellular Archives. 2022; 1;22(1): 1793-1800. https://connectjournals.com/03896.2022.22.1793
  • [3] Damiani G, Eggenhöffner R, Pigatto PDM, Bragazzi NL. Nanotechnology meets atopic dermatitis: Current solutions, challenges and future prospects. Insights and implications from a systematic review of the literature. Bioact Mater. 2019;4:380-386. https://doi.org/10.1016/j.bioactmat.2019.11.003
  • [4] Saliem AH, Ibrahim OM, Salih SI. Biosynthesis of silver nanoparticles using Cinnamon zeylanicum plants bark extract. Kufa journal for veterinary medical sciences, 2016;7(1): 51-63
  • [5] Botteon CEA, Silva LB, Ccana-Ccapatinta GV, Silva TS, Ambrosio SR, Veneziani RCS, Bastos JK, Marcato PD. Biosynthesis and characterization of gold nanoparticles using Brazilian red propolis and evaluation of its antimicrobial and anticancer activities. Sci Rep. 2021;11(1):1974. https://doi.org/10.1038/s41598-021-81281-w
  • [6] Abdulsattar SA. Biological impact of gold nanoparticle on estradaiol and testosterone levels in sera of human males. Journal of the Faculty of Medicine Baghdad. 2015;57(4):333-7. https://doi.org/10.32007/jfacmedbagdad.574403
  • [7] Al-Shaabani MJM, Al-Ethawi AMT, Al-Mathkhury HJF. Eco-friendly synthesis of gold nanoparticles and study their effect with antibiotics against Acinetobacter baumannii. Iraqi Journal of Agricultural Sciences 2020;51(4): 1204-1211. https://doi.org/10.36103/ijas.v51i4.1099
  • [8] Ayad ZM, Ibrahim OMS, Omar LW. Biosynthesis and characterization of silver nanoparticles by Silybum marianum (silymarin) fruit extract. Adv. Anim. Vet. Sci. 2019; 7(2):122-130. http://dx.doi.org/10.17582/journal.aavs/2019/7.2.122.130
  • [9] Arslan, K. (2023). The Antioxidant, Antimicrobial, and Total Phenolic Potential of Clove Extracts for Inhibition of Food Pathogens. Erzincan University Journal of Science and Technology, 16(2), 453-464. https://doi.org/10.18185/erzifbed.1279953
  • [10] Ayoola GA, Lawore FM, Adelowotan T, Aibinu IE, Adenipekun E, Coker HAB, Odugbemi, TO. Chemical analysis and antimicrobial activity of the essential oil of Syzigium aromaticum (clove). Afr J Microbiol Res, 2008;2(7): 162-166.
  • [11] Ali BM, Ibrahim O. Antifungal Activity of Clove (Syzygium aromaticum) Essential Oil Extract against Induced Topical Skin Infection by Candida albicans in Mice in vivo. The Egyptian Journal of Hospital Medicine. 2023;91(1):3855-61. https://dx.doi.org/10.21608/ejhm.2023.293468
  • [12] Kapadiya S, Desai MA. Isolation of essential oil from buds of Syzygium aromaticum using hydrodistillation: multi response optimization and predictive modeling, International Journal of Advance Research in Science and Engineering. 2017;6 (1).
  • [13] Bhuiyan MNI, Begum J, Nandi NC, Akter F. Constituents of the essential oil from leaves and buds of clove (Syzigium caryophyllatum (L.) Alston). African Journal of Plant Science, 2010;4(11): 451-454.
  • [14] Razafimamonjison G, Jahiel M, Duclos T, Ramanoelina P, Fawbush F, Danthu P. Bud, leaf, and stem essential oil composition of Syzygium aromaticum from Madagascar, Indonesia, and Zanzibar. International Journal of Basic and Applied Sciences, 2014;3(3): 224-233. http://dx.doi.org/10.14419/ijbas.v3i3.2473
  • [15] Takahashi H, Nakamura A, Fujino N, Sawaguchi Y, Sato M, Kuda T, Kimura B. Evaluation of the antibacterial activity of allyl isothiocyanate, clove oil, eugenol and carvacrol against spoilage lactic acid bacteria. LWT. 2021;145:111263.https://doi.org/10.1016/j.lwt.2021.111263
  • [16] Zari AT, Zari TA, Hakeem KR. Anticancer Properties of Eugenol: A Review. Molecules. 2021;26(23):7407. https://doi.org/10.3390/molecules26237407
  • [17] Safrudin I, Maimulyanti A, Prihadi AR. Effect of crushing of clove bud (Syzygium aromaticum) and distillation rate on main constituents of the essential oil. American Journal of Essential Oils and Natural Products. 2015;2(3):12-5.
  • [18] Gaspar EM, Duarte, R, & Santana JC. Volatile composition and antioxidant properties of clove products. Biomedical Journal of Scientific and Technical Research, 2018; 9(4), 7270-7276. http://dx.doi.org/10.26717/BJSTR.2018.09.001831
  • [19] Lee S, Najiah M, Wendy W, Nadirah M. Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites. Frontiers of Agriculture in China, 2009;3: 332-336. http://dx.doi.org/10.1007/s11703-009-0052-8
  • [20] Selles SMA, Kouidri M, Belhamiti BT, Ait Amrane A. Chemical composition, in-vitro antibacterial and antioxidant activities of Syzygium aromaticum essential oil. Food Measure. 2020;14(4):2352–8. https://doi.org/10.1007%2Fs11694 020-00482-5
  • [21] Nee Kamaldeep KS, Kaur S, Bhalla V, Kumar M, Gupta A. Pentacenequinone derivatives for the preparation of gold nanoparticles: facile synthesis and catalytic application. Journal of Materials Chemistry A, 2014;2(22): 8369-8375. https://doi.org/10.1039/C4TA00397G
  • [22] Basavaraja S, Balaji SD, Lagashetty A, Rajasab AH, Venkataraman A. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Materials Research Bulletin, 2008;43(5): 1164-1170. http://dx.doi.org/10.1016/j.materresbull.2007.06.020
  • [23] Carone A, Emilsson S, Mariani P, Désert A, Parola S. Gold nanoparticle shape dependence of colloidal stability domains. Nanoscale Adv. 2023;5(7):2017-2026. https://doi.org/10.1039%2Fd2na00809b
  • [24] Ebrahimzadeh MA, Naghizadeh A, Mohammadi-Aghdam S, Khojasteh H, Ghoreishi SM, Mortazavi-Derazkola S. Enhanced catalytic and antibacterial efficiency of biosynthesized Convolvulus fruticosus extract capped gold nanoparticles (CFE@AuNPs). J Photochem Photobiol B. 2020;209:111949. https://doi.org/10.1016/j.jphotobiol.2020.111949
  • [25] Siddique S, Chow JC. Gold nanoparticles for drug delivery and cancer therapy. Applied Sciences, 2020;10(11): 3824. https://doi.org/10.3390/app10113824
  • [26] Vilas V, Philip D, Mathew J. Biosynthesis of Au and Au/Ag alloy nanoparticles using Coleus aromaticus essential oil and evaluation of their catalytic, antibacterial and antiradical activities. Journal of Molecular Liquids,2016; 221: 179-189. http://dx.doi.org/10.1016/j.molliq.2016.05.066
  • [27] Meena Kumari M, Philip D. Facile one-pot synthesis of gold and silver nanocatalysts using edible coconut oil. Spectrochim Acta A Mol Biomol Spectrosc. 2013;111:154-60. https://doi.org/10.1016/j.saa.2013.03.076
  • [28] Do PQT, Huong VT, Phuong NTT, Nguyen TH, Ta HKT, Ju H, Phan TB, Phung VD, Trinh KTL, Tran NHT. The highly sensitive determination of serotonin by using gold nanoparticles (Au NPs) with a localized surface plasmon resonance (LSPR) absorption wavelength in the visible region. RSC Adv. 2020;10(51):30858-30869. https://doi.org/10.1039%2Fd0ra05271j
  • [29] Husain WM, Araak JK, Ibrahim OMS. Effect of different doses from znonps on the pituitary-testes axis function in adult male rats.2019. Adv. Anim. Vet. Sci, 7(7), 550-556. http://dx.doi.org/10.17582/journal.aavs/2019/7.7.550.556
  • [30] Hussein SI, Shubber SS, Yaseen NY. Bio-distribution of Gold Nanoparticles in Tumor Mass and Different Organs in Implanted Mice with Mammary Adenocarcinoma AM3 (in vivo study). The Iraqi Journal of Veterinary Medicine. 2019 Dec 28;43(2):17-22. https://doi.org/10.30539/iraqijvm.v43i2.525.
  • [31] Pramod SK, Navnath, KA, Pramod SM. A revıew on gas chromatography-mass spectrometry (GC-MS). World J. Pharm. Res. 2021; 10(3): 741-763.https://doi.org/10.17605/OSF.IO/GAFMT
  • [32] Yassin MT, Mostafa AAF, Al-Askar AA. In vitro, anticandidal potency of Syzygium aromaticum (clove) extracts against vaginal candidiasis. BMC complementary medicine and therapies, 2020;20(1): 1-9. https://doi.org/10.1186/s12906-020-2818-8
  • [33] Singh AK, Talat M, Singh DP, Srivastava ON. Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with an amine group. Journal of Nanoparticle Research, 2010; 12, 1667-1675. http://dx.doi.org/10.1007/s11051-009-9835-3
  • [34] Azam A, Ahmed F, Arshi N, Chaman M, Naqvi AH. One-step synthesis and characterization of gold nanoparticles and their antibacterial activities against E. coli (ATCC 25922 strain). Int J Theor Appl Sci, 2009;1(2): 1-4.
  • [35] Rasmussen MK, Pedersen JN, Marie R. Size and surface charge characterization of nanoparticles with a salt gradient. Nat Commun. 2020;11(1):2337. https://doi.org/10.1038/s41467-020-15889-3
  • [36] Ghosh P, Han G, De M, Kim CK, Rotello VM. Gold nanoparticles in delivery applications. Adv Drug Deliv Rev. 2008;60(11):1307-1315. https://doi.org/10.1016/j.addr.2008.03.016
  • [37] Bennur T, Khan Z, Kshirsagar R, Javdekar V, Zinjarde S. Biogenic gold nanoparticles from the Actinomycete Gordonia amarae: application in rapid sensing of copper ions. Sensors and Actuators B: Chemical, 2016;233: 684-690. http://dx.doi.org/10.1016/j.snb.2016.04.022
  • [38] Ankamwar B. Biosynthesis of gold nanoparticles (green-gold) using leaf extract of Terminalia catappa. E-Journal of Chemistry, 2010;7(4):1334-1339. https://doi.org/10.1155/2010/745120
There are 38 citations in total.

Details

Primary Language English
Subjects Pharmacy Management
Journal Section Research Article
Authors

Humam T. Hadi This is me 0000-0001-8875-1631

Orooba Mohammed Saeed Ibrahim This is me 0000-0002-0682-8621

Publication Date June 28, 2025
DOI https://doi.org/10.29228/jrp.861
IZ https://izlik.org/JA56SE53AX
Published in Issue Year 2024 Volume: 28 Issue: 6

Cite

APA T. Hadi, H., & Ibrahim, O. M. S. (2025). Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation. Journal of Research in Pharmacy, 28(6), 1883-1891. https://doi.org/10.29228/jrp.861
AMA 1.T. Hadi H, Ibrahim OMS. Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation. J. Res. Pharm. 2025;28(6):1883-1891. doi:10.29228/jrp.861
Chicago T. Hadi, Humam, and Orooba Mohammed Saeed Ibrahim. 2025. “Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium Aromaticum) Oil Extracted by Hydrodistillation”. Journal of Research in Pharmacy 28 (6): 1883-91. https://doi.org/10.29228/jrp.861.
EndNote T. Hadi H, Ibrahim OMS (July 1, 2025) Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation. Journal of Research in Pharmacy 28 6 1883–1891.
IEEE [1]H. T. Hadi and O. M. S. Ibrahim, “Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation”, J. Res. Pharm., vol. 28, no. 6, pp. 1883–1891, July 2025, doi: 10.29228/jrp.861.
ISNAD T. Hadi, Humam - Ibrahim, Orooba Mohammed Saeed. “Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium Aromaticum) Oil Extracted by Hydrodistillation”. Journal of Research in Pharmacy 28/6 (July 1, 2025): 1883-1891. https://doi.org/10.29228/jrp.861.
JAMA 1.T. Hadi H, Ibrahim OMS. Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation. J. Res. Pharm. 2025;28:1883–1891.
MLA T. Hadi, Humam, and Orooba Mohammed Saeed Ibrahim. “Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium Aromaticum) Oil Extracted by Hydrodistillation”. Journal of Research in Pharmacy, vol. 28, no. 6, July 2025, pp. 1883-91, doi:10.29228/jrp.861.
Vancouver 1.T. Hadi H, Ibrahim OMS. Green Synthesis and Characterization of Gold Nanoparticles Using Crushed Clove Buds (Syzygium aromaticum) Oil Extracted by Hydrodistillation. J. Res. Pharm. [Internet]. 2025 July 1;28(6):1883-91. Available from: https://izlik.org/JA56SE53AX