Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells

Tina Rostinawati; Ami Tjitraresmi; Nyi Mekar Saptarini; Rahma Ziska; Riezki Amalia; Aisyah Sadira Azalia; Harmony Anastasya Nababan

doi:10.12991/jrespharm.1636574

Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells

Abstract

L-Asparaginase (L-Asp) is an enzyme utilized for treatment of acute lymphoblastic leukemia. The current bacterial L-Asp therapy often leads to undesirable side effects. L-Asp derived from plants of the Solanaceae and Fabaceae families may offer reduced side effects. The study aims to identify plants from these families with L-Asp activity. Initially, 11 plant species were selected: Crude protein was extracted using a 1 M potassium phosphate buffer at pH 8.6, with a plant-to-buffer ratio of 1:2. The extract was assessed for L-Asp activity using the Nessler reaction and protein content was also determined. The crude enzyme's specific activity was calculated, identifying one extract as a promising L-Asp candidate. Subsequently, optimal conditions for L-Asp activity were determined and isolation of L-Asp candidates involved salting out, dialysis, chromatography, Native PAGE, passive elution, enzyme activity and anticancer assay. The Archidendron pauciflorum seed extract exhibited significant characteristics: crude protein content of 237.08 µg/mL, L-Asp activity of 242.84 Units/mL, and specific activity of 1,024 Units/µg. Optimal activity was observed at 40⁰C, using 0.5 M potassium phosphate buffer at pH 8.8. Protein isolation indicated the L-Asp candidate's molecular weight ± 70 kDa, with activity and specific activity values of 240.74 Units/mL and 0.129 Units/µg. Anticancer assay showed that the acyive protein induced cytotoxic effects on MCF-7 and A549 cancer cells with an IC50 value of 6.43 and 8.07 U/ml. The cytotoxic activity of the active protein against these two types of cancer cells is classified as moderate and has potential as an anti-cancer agent.

Keywords

Thanks

This research was funded by Ministry of Education, Culture, Research, and Technolog, Imdonesia with contract number 148/E5/PG.02.00.PL/2023

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Details

Primary Language

English

Subjects

Pharmaceutical Biotechnology, Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Tina Rostinawati ^*
0000-0002-7139-8250
Indonesia

Ami Tjitraresmi
0000-0002-7990-0567
Indonesia

Nyi Mekar Saptarini
0000-0002-2406-9411
Indonesia

Rahma Ziska
0000-0001-6939-0535
Indonesia

Riezki Amalia
0000-0002-8445-7045
Indonesia

Aisyah Sadira Azalia
0009-0005-7257-266X
Indonesia

Harmony Anastasya Nababan
0009-0001-4337-9650
Indonesia

Publication Date

March 15, 2026

Submission Date

February 11, 2025

Acceptance Date

June 28, 2025

Published in Issue

Year 2026 Volume: 30 Number: 2

DOI

https://doi.org/10.12991/jrespharm.1636574

IZ

https://izlik.org/JA65ND54PE

Cite

RIS / Bibtex

APA

Rostinawati, T., Tjitraresmi, A., Saptarini, N. M., Ziska, R., Amalia, R., Azalia, A. S., & Nababan, H. A. (2026). Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells. Journal of Research in Pharmacy, 30(2), 467-481. https://doi.org/10.12991/jrespharm.1636574

AMA

1.Rostinawati T, Tjitraresmi A, Saptarini NM, et al. Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells. J. Res. Pharm. 2026;30(2):467-481. doi:10.12991/jrespharm.1636574

Chicago

Rostinawati, Tina, Ami Tjitraresmi, Nyi Mekar Saptarini, et al. 2026. “Sreening of L-Asparaginase from Indigenous Indonesian Plants As a Potential Anticancer Agent to Mcf-7 Breast and A549 Lung Cancer Cells”. Journal of Research in Pharmacy 30 (2): 467-81. https://doi.org/10.12991/jrespharm.1636574.

EndNote

Rostinawati T, Tjitraresmi A, Saptarini NM, Ziska R, Amalia R, Azalia AS, Nababan HA (March 1, 2026) Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells. Journal of Research in Pharmacy 30 2 467–481.

IEEE

[1]T. Rostinawati et al., “Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells”, J. Res. Pharm., vol. 30, no. 2, pp. 467–481, Mar. 2026, doi: 10.12991/jrespharm.1636574.

ISNAD

Rostinawati, Tina - Tjitraresmi, Ami - Saptarini, Nyi Mekar - Ziska, Rahma - Amalia, Riezki - Azalia, Aisyah Sadira - Nababan, Harmony Anastasya. “Sreening of L-Asparaginase from Indigenous Indonesian Plants As a Potential Anticancer Agent to Mcf-7 Breast and A549 Lung Cancer Cells”. Journal of Research in Pharmacy 30/2 (March 1, 2026): 467-481. https://doi.org/10.12991/jrespharm.1636574.

JAMA

1.Rostinawati T, Tjitraresmi A, Saptarini NM, Ziska R, Amalia R, Azalia AS, Nababan HA. Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells. J. Res. Pharm. 2026;30:467–481.

MLA

Rostinawati, Tina, et al. “Sreening of L-Asparaginase from Indigenous Indonesian Plants As a Potential Anticancer Agent to Mcf-7 Breast and A549 Lung Cancer Cells”. Journal of Research in Pharmacy, vol. 30, no. 2, Mar. 2026, pp. 467-81, doi:10.12991/jrespharm.1636574.

Vancouver

1.Tina Rostinawati, Ami Tjitraresmi, Nyi Mekar Saptarini, Rahma Ziska, Riezki Amalia, Aisyah Sadira Azalia, Harmony Anastasya Nababan. Sreening of L-Asparaginase from indigenous Indonesian plants as a potential anticancer agent to Mcf-7 breast and A549 lung cancer cells. J. Res. Pharm. 2026 Mar. 1;30(2):467-81. doi:10.12991/jrespharm.1636574