Protective effect of edaravone on rat testis after valproic acid treatment

Çağrı Çelik; Bertan Boran Bayrak; Neziha Hacıhasanoğlu Çakmak; Refiye Yanardağ

Protective effect of edaravone on rat testis after valproic acid treatment

Abstract

Valproic acid (n-dipropyl-acetic acid, VPA) is a medication used as anticonvulsant in the treatment of bipolar disorder, and for migraine prophylaxis. Long-term use of VPA is known to trigger reproductive impairment, which is mediated by elevation of testicular oxidative stress. Edaravone is used in the treatment of cerebrovascular diseases. It can diffuse into many disease-affected organs, thus shows protective effects in numerous tissues including the heart, lung, and testis. The main goal of the present study was to determine the possible protective role of edaravone against VPA-induced oxidative testicular injury. Male Sprague Dawley rats were assigned into four groups. Control rats; rats given only edaravone (30 mg/kg/day); rats given only VPA (500 mg/kg/day); rats given VPA+edaravone for seven days. Edaravone and VPA were applied intraperitoneally. After eight days, testicular tissues were taken from rats. There was a statistically significant increase in the levels of reduced glutathione, lipid peroxidation, reactive oxygen species, total oxidant status, oxidative stress index, and DNA contents as well as catalase, superoxide dismutase, glutathione-related enzymes, gamma-glutamyl transferase, acid and alkaline phosphatases, lactate dehydrogenase, myeloperoxidase and sorbitol dehydrogenase activities in VPA group. More so, advanced oxidized protein products, protein carbonyl, and nitric oxide levels were also significantly increased in VPA group. Activities of glucose-6-phosphate dehydrogenase and sodium/potassium ATPase and total antioxidant status levels remarkably decreased in VPA given group. Treatment with edaravone to VPA group significantly reverted these alterations. These findings demonstrate that administration of edaravone has a beneficial effect against testicular injury in VPA-induced oxidative stress.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Sciences

Journal Section

Research Article

Authors

Çağrı Çelik This is me
Türkiye

Bertan Boran Bayrak ^*
Türkiye

Neziha Hacıhasanoğlu Çakmak
Türkiye

Refiye Yanardağ
Türkiye

Publication Date

June 28, 2025

Submission Date

July 27, 2021

Acceptance Date

September 4, 2021

Published in Issue

Year 2022 Volume: 26 Number: 1

IZ

https://izlik.org/JA66AJ88ER

Cite

RIS / Bibtex

APA

Çelik, Ç., Bayrak, B. B., Hacıhasanoğlu Çakmak, N., & Yanardağ, R. (2025). Protective effect of edaravone on rat testis after valproic acid treatment. Journal of Research in Pharmacy, 26(1), 52-62. https://izlik.org/JA66AJ88ER

AMA

1.Çelik Ç, Bayrak BB, Hacıhasanoğlu Çakmak N, Yanardağ R. Protective effect of edaravone on rat testis after valproic acid treatment. J. Res. Pharm. 2025;26(1):52-62. https://izlik.org/JA66AJ88ER

Chicago

Çelik, Çağrı, Bertan Boran Bayrak, Neziha Hacıhasanoğlu Çakmak, and Refiye Yanardağ. 2025. “Protective Effect of Edaravone on Rat Testis After Valproic Acid Treatment”. Journal of Research in Pharmacy 26 (1): 52-62. https://izlik.org/JA66AJ88ER.

EndNote

Çelik Ç, Bayrak BB, Hacıhasanoğlu Çakmak N, Yanardağ R (June 1, 2025) Protective effect of edaravone on rat testis after valproic acid treatment. Journal of Research in Pharmacy 26 1 52–62.

IEEE

[1]Ç. Çelik, B. B. Bayrak, N. Hacıhasanoğlu Çakmak, and R. Yanardağ, “Protective effect of edaravone on rat testis after valproic acid treatment”, J. Res. Pharm., vol. 26, no. 1, pp. 52–62, June 2025, [Online]. Available: https://izlik.org/JA66AJ88ER

ISNAD

Çelik, Çağrı - Bayrak, Bertan Boran - Hacıhasanoğlu Çakmak, Neziha - Yanardağ, Refiye. “Protective Effect of Edaravone on Rat Testis After Valproic Acid Treatment”. Journal of Research in Pharmacy 26/1 (June 1, 2025): 52-62. https://izlik.org/JA66AJ88ER.

JAMA

1.Çelik Ç, Bayrak BB, Hacıhasanoğlu Çakmak N, Yanardağ R. Protective effect of edaravone on rat testis after valproic acid treatment. J. Res. Pharm. 2025;26:52–62.

MLA

Çelik, Çağrı, et al. “Protective Effect of Edaravone on Rat Testis After Valproic Acid Treatment”. Journal of Research in Pharmacy, vol. 26, no. 1, June 2025, pp. 52-62, https://izlik.org/JA66AJ88ER.

Vancouver

1.Çağrı Çelik, Bertan Boran Bayrak, Neziha Hacıhasanoğlu Çakmak, Refiye Yanardağ. Protective effect of edaravone on rat testis after valproic acid treatment. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(1):52-6. Available from: https://izlik.org/JA66AJ88ER