Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice

Maedeh Hamzeh; Fereshteh Talebpour Amiri; Saeed Yaghubi Beklar; Seyed Jalal Hosseinimehr

Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice

Abstract

Cyclophosphamide [CP], as alkylating agents has side effects such as nephrotoxicity. Cerium oxide nanoparticles [nanoceria; NC], as an antioxidant, are effective at reduction oxidative stress. This study evaluated the protective effect of nanoceria in nephrotoxicity induced CP. 32 BALB/c mice were randomly divided into four equal groups. Control, NC, CP and NC+CP. NC and CP injected intraperitoneally respectively in dose of 100 μg/kg for 3 days and 200 mg/kg single dose on 3th day of study. Two days after the final treatment, histochemical, serum biochemical, histopathological and immunohistochemical examinations were performed for determination effects of NC on nephrotoxicity. Oxidative stress and renal injury induced in CP treated mice were proved by the significantly elevation of urea and creatinine and alteration in oxidative stress markers [MDA and GSH levels]. Consequently, histopathological changes and apoptosis were markedly increased. NC was able to reduce MDA, urea , creatinine and increase GSH content. In addition, NC pretreatment could alleviated immunoreactivity of caspase-3. NC revealed a strong antioxidant in nephrotoxicity following CP treatment. This study suggests that NC through antioxidant and antiapoptotic properties have protective effect against CP-induced nephrotoxicity.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Maedeh Hamzeh This is me
0000-0003-3907-8772
Iran

Fereshteh Talebpour Amiri ^* This is me
0000-0001-9847-3401
Iran

Saeed Yaghubi Beklar This is me
Iran

Seyed Jalal Hosseinimehr This is me
0000-0001-8055-8036
Iran

Publication Date

June 27, 2025

Submission Date

November 18, 2017

Acceptance Date

December 21, 2017

Published in Issue

Year 2018 Volume: 22 Number: 2

IZ

https://izlik.org/JA24RY25DH

Cite

RIS / Bibtex

APA

Hamzeh, M., Talebpour Amiri, F., Beklar, S. Y., & Hosseinimehr, S. J. (2025). Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice. Journal of Research in Pharmacy, 22(2), 180-189. https://izlik.org/JA24RY25DH

AMA

1.Hamzeh M, Talebpour Amiri F, Beklar SY, Hosseinimehr SJ. Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice. J. Res. Pharm. 2025;22(2):180-189. https://izlik.org/JA24RY25DH

Chicago

Hamzeh, Maedeh, Fereshteh Talebpour Amiri, Saeed Yaghubi Beklar, and Seyed Jalal Hosseinimehr. 2025. “Nephroprotective Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Nephrotoxicity via Antiapoptotic and Antioxidant Properties in BALB C Mice”. Journal of Research in Pharmacy 22 (2): 180-89. https://izlik.org/JA24RY25DH.

EndNote

Hamzeh M, Talebpour Amiri F, Beklar SY, Hosseinimehr SJ (June 1, 2025) Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice. Journal of Research in Pharmacy 22 2 180–189.

IEEE

[1]M. Hamzeh, F. Talebpour Amiri, S. Y. Beklar, and S. J. Hosseinimehr, “Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice”, J. Res. Pharm., vol. 22, no. 2, pp. 180–189, June 2025, [Online]. Available: https://izlik.org/JA24RY25DH

ISNAD

Hamzeh, Maedeh - Talebpour Amiri, Fereshteh - Beklar, Saeed Yaghubi - Hosseinimehr, Seyed Jalal. “Nephroprotective Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Nephrotoxicity via Antiapoptotic and Antioxidant Properties in BALB C Mice”. Journal of Research in Pharmacy 22/2 (June 1, 2025): 180-189. https://izlik.org/JA24RY25DH.

JAMA

1.Hamzeh M, Talebpour Amiri F, Beklar SY, Hosseinimehr SJ. Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice. J. Res. Pharm. 2025;22:180–189.

MLA

Hamzeh, Maedeh, et al. “Nephroprotective Effect of Cerium Oxide Nanoparticles on Cyclophosphamide-Induced Nephrotoxicity via Antiapoptotic and Antioxidant Properties in BALB C Mice”. Journal of Research in Pharmacy, vol. 22, no. 2, June 2025, pp. 180-9, https://izlik.org/JA24RY25DH.

Vancouver

1.Maedeh Hamzeh, Fereshteh Talebpour Amiri, Saeed Yaghubi Beklar, Seyed Jalal Hosseinimehr. Nephroprotective effect of cerium oxide nanoparticles on cyclophosphamide-induced nephrotoxicity via antiapoptotic and antioxidant properties in BALB/c mice. J. Res. Pharm. [Internet]. 2025 Jun. 1;22(2):180-9. Available from: https://izlik.org/JA24RY25DH