Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf.

Samira Mirzaeidarband; Sara Samadishams; Sanaz Hamedeyazdan; Zahra Eskandari; Atefeh Ebrahimi; Sedigheh Bamdad—moghaddam; Jaleh Barar; Yadollah Omidi; Laleh Khodaie; Hossein Nazemiyeh

Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf.

Abstract

Gleditsia caspica Desf. (Caesalpinaceae) is traditionally used to treat diverse diseases. This research paper aims to investigate anti-proliferative properties and phytochemical compounds of methanolic extract from the pods of G. caspica. MTT test was used to measure in vitro anti-proliferative effect of the soxhlet-extracted methanolic extract of G.caspica pods with the concentration ranging from 25-1000 µg/ml against A549 cell line. The extract was applied to SPE, HPLC preparative system, and spectral analysis (1HNMR, 13CNMR, and UV) for fractionation, purification, and identification of phytoconstituents, respectively. The results indicated that the extract prevented proliferation of A549 cell line dependent on time and concentration. After 48 h and 72 h, there was a significant decrease in cell viability initiated from 100 and 500 (µg/ml) concentrations in comparison to untreated control. Phytochemical assessment of the extract resulted in two alkaloids, locustoside A and saikachinoside A, and four flavonoids quercetin-3,7-diglucoside, luteolin-4',7–diglucoside, quercetin-3-glucoside, and quercetin. This experiment was an activity-guided phytochemical study. In prior studies, quercetin and quercetin-3-β-D-glucoside showed in vitro inhibitory effect on proliferation of A549 cell line. However, the cytotoxic properties of the other identified compounds have not been reported. Cytotoxic properties of G.caspica was previously attributed to saponins, while isolated flavonoids and alkaloids could be responsible for the extract’s cytotoxic effects. This paper first explored cytotoxic activity of methanolic extract from the pods of G.caspica on A549 cell line and identified flavonoids and alkaloids of this plant.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Samira Mirzaeidarband This is me
0009-0007-2572-1962
Iran

Sara Samadishams This is me
0009-0009-1713-4009
Iran

Sanaz Hamedeyazdan This is me
0000-0002-8950-9879
Iran

Zahra Eskandari This is me
0009-0001-8455-2237
Iran

Atefeh Ebrahimi This is me
0000-0003-0172-1283
Iran

Sedigheh Bamdad—moghaddam This is me
0000-0002-7320-8908
Iran

Jaleh Barar This is me
0000-0002-0105-919X
Iran

Yadollah Omidi This is me
0000-0003-0067-2475
Iran

Laleh Khodaie ^*
0000-0001-7416-0822
Iran

Hossein Nazemiyeh This is me
0000-0003-0165-3209
Iran

Publication Date

June 28, 2025

Submission Date

August 22, 2023

Acceptance Date

October 23, 2023

Published in Issue

Year 2024 Volume: 28 Number: 1

IZ

https://izlik.org/JA39EF36NU

Cite

RIS / Bibtex

APA

Mirzaeidarband, S., Samadishams, S., Hamedeyazdan, S., Eskandari, Z., Ebrahimi, A., Bamdad—moghaddam, S., Barar, J., Omidi, Y., Khodaie, L., & Nazemiyeh, H. (2025). Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. Journal of Research in Pharmacy, 28(1), 304-312. https://izlik.org/JA39EF36NU

AMA

1.Mirzaeidarband S, Samadishams S, Hamedeyazdan S, et al. Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. J. Res. Pharm. 2025;28(1):304-312. https://izlik.org/JA39EF36NU

Chicago

Mirzaeidarband, Samira, Sara Samadishams, Sanaz Hamedeyazdan, et al. 2025. “Anti-Proliferative Activity and Phytochemical Study of the Methanolic Extract from the Pods of Gleditsia Caspica Desf”. Journal of Research in Pharmacy 28 (1): 304-12. https://izlik.org/JA39EF36NU.

EndNote

Mirzaeidarband S, Samadishams S, Hamedeyazdan S, Eskandari Z, Ebrahimi A, Bamdad—moghaddam S, Barar J, Omidi Y, Khodaie L, Nazemiyeh H (June 1, 2025) Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. Journal of Research in Pharmacy 28 1 304–312.

IEEE

[1]S. Mirzaeidarband et al., “Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf”., J. Res. Pharm., vol. 28, no. 1, pp. 304–312, June 2025, [Online]. Available: https://izlik.org/JA39EF36NU

ISNAD

Mirzaeidarband, Samira - Samadishams, Sara - Hamedeyazdan, Sanaz - Eskandari, Zahra - Ebrahimi, Atefeh - Bamdad—moghaddam, Sedigheh - Barar, Jaleh - Omidi, Yadollah - Khodaie, Laleh - Nazemiyeh, Hossein. “Anti-Proliferative Activity and Phytochemical Study of the Methanolic Extract from the Pods of Gleditsia Caspica Desf”. Journal of Research in Pharmacy 28/1 (June 1, 2025): 304-312. https://izlik.org/JA39EF36NU.

JAMA

1.Mirzaeidarband S, Samadishams S, Hamedeyazdan S, Eskandari Z, Ebrahimi A, Bamdad—moghaddam S, Barar J, Omidi Y, Khodaie L, Nazemiyeh H. Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. J. Res. Pharm. 2025;28:304–312.

MLA

Mirzaeidarband, Samira, et al. “Anti-Proliferative Activity and Phytochemical Study of the Methanolic Extract from the Pods of Gleditsia Caspica Desf”. Journal of Research in Pharmacy, vol. 28, no. 1, June 2025, pp. 304-12, https://izlik.org/JA39EF36NU.

Vancouver

1.Samira Mirzaeidarband, Sara Samadishams, Sanaz Hamedeyazdan, Zahra Eskandari, Atefeh Ebrahimi, Sedigheh Bamdad—moghaddam, Jaleh Barar, Yadollah Omidi, Laleh Khodaie, Hossein Nazemiyeh. Anti-proliferative activity and phytochemical study of the methanolic extract from the pods of Gleditsia caspica Desf. J. Res. Pharm. [Internet]. 2025 Jun. 1;28(1):304-12. Available from: https://izlik.org/JA39EF36NU