High Performance Liquid Chromatography Method Validation and Forced Degradation Studies of Chrysin

Year 2023, Volume: 27 Issue: 1, 264 - 275, 28.06.2025

Kanchan Nikam Sachin Bhusari Pravin Wakte

https://izlik.org/JA96LG43MB

Abstract

Chrysin is belonging to flavone class. It is found in Passiflora incarnata, Oroxylum indicum, mashroom, honey and propolis. An isocratic LC method was developed, optimized and validated for Chrysin. The method was validated accord¬ing the Q2 (R1) guidelines of ICH with respect to linearity, range, system suitability, LOD, LOQ, accuracy, precision. Chromatographic separation were performed on Agilent EC-C-18 column (100 x 4.6 mm, 2.7 um). The mobile phase composed of 0.1% acetic acid in Water: Methanol (25:75 v/v). The flow rate was 0.8 mL/min. The developed method was applied to the different extracts of Passiflora incarnata for estimation of Chrysin using advanced extraction techniques. Chrysin was also estimated in marketed formulation. The retention time for Chrysin was 2.57 min. the calibration curve of Chrysin was linear (R2-0.999) in the concentration range of 2-20 ng/ml. The inter-day accuracy ranged -0.52 to 4.77 % and intra-day accuracy ranged from -0.45 to 4.88%. The interday precision was from 0.368 to 1.68 % and intraday precision was ranges from 0.0121 to 1.35 %. The limits of detection and quantification were 0.004748 and 0.014244 respectively. Chrysin showed significant degradation when exposed to water, acid, base, oxidizing agent, and UV light. Chrysin was estimated in Passiflora incarnata leaves extracted by different extraction techniques viz. SAE, UAE, ASE and in Marketed formulation. An accurate, precise and novel HPLC method was developed and validated. The method was successfully used to estimate the Chrysin in Passiflora incarnata extracts and its marketed formulation.

Keywords

Chrysin , HPLC method development , Validation , Forced degradation studies

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