Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method

Hajer Aswaissi; Ebru Türköz Acar

Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method

Abstract

In this study, a new high performance liquid chromatography (HPLC) analysis method was developed for the simultaneous determination of flurbiprofen (F) and thiocolchicoside (T) in pharmaceutical products. A C18 column (Agilent Poroshell 120 EC-C18) was used as the stationary phase. Elution of the analytes was achieved by using gradient elution system. Mobile phase A was an acetate buffer (100 mM, pH 4.8) and mobile phase B was acetonitrile. The flow rate for the method developed was 0.5 ml/min. The temperature of the column compartment was set at 40˚C and the injection volume was 5 μl. Monitoring of the analytes was carried out at 248 nm. The method was found linear in the concentration range between 5-100 ppm. The correlation coefficient value was calculated as 0.999 for F and 0.999 for T. The method was found suitable in terms of accuracy, precision, specificity, linearity, and robustness ruggedness. Furthermore, it was applied successfully for the analysis of commercial tablets and gel samples that contains F and T without any time-consuming pre-procedure. The recovery values for Fand T were found as 99.5% and 97.0% in tablet formulations and 98.6% and 99.3% in gel formulations, respectively. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry, Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Hajer Aswaissi This is me
0000-0001-8472-8143
Türkiye

Ebru Türköz Acar ^*
0000-0002-8164-6617
Türkiye

Publication Date

June 28, 2025

Submission Date

December 10, 2021

Acceptance Date

April 8, 2022

Published in Issue

Year 2022 Volume: 26 Number: 3

IZ

https://izlik.org/JA78UC57DA

Cite

RIS / Bibtex

APA

Aswaissi, H., & Türköz Acar, E. (2025). Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method. Journal of Research in Pharmacy, 26(3), 663-674. https://izlik.org/JA78UC57DA

AMA

1.Aswaissi H, Türköz Acar E. Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method. J. Res. Pharm. 2025;26(3):663-674. https://izlik.org/JA78UC57DA

Chicago

Aswaissi, Hajer, and Ebru Türköz Acar. 2025. “Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method”. Journal of Research in Pharmacy 26 (3): 663-74. https://izlik.org/JA78UC57DA.

EndNote

Aswaissi H, Türköz Acar E (June 1, 2025) Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method. Journal of Research in Pharmacy 26 3 663–674.

IEEE

[1]H. Aswaissi and E. Türköz Acar, “Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method”, J. Res. Pharm., vol. 26, no. 3, pp. 663–674, June 2025, [Online]. Available: https://izlik.org/JA78UC57DA

ISNAD

Aswaissi, Hajer - Türköz Acar, Ebru. “Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method”. Journal of Research in Pharmacy 26/3 (June 1, 2025): 663-674. https://izlik.org/JA78UC57DA.

JAMA

1.Aswaissi H, Türköz Acar E. Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method. J. Res. Pharm. 2025;26:663–674.

MLA

Aswaissi, Hajer, and Ebru Türköz Acar. “Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method”. Journal of Research in Pharmacy, vol. 26, no. 3, June 2025, pp. 663-74, https://izlik.org/JA78UC57DA.

Vancouver

1.Hajer Aswaissi, Ebru Türköz Acar. Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method. J. Res. Pharm. [Internet]. 2025 Jun. 1;26(3):663-74. Available from: https://izlik.org/JA78UC57DA