Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet

Putra Santoso; Wilka Ramadhia; Resti Rahayu; Rita Maliza

Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet

Abstract

Yam bean (Pachyrhizus erosus L.) is a prominent medicinal plant exerting various health benefits. However, it remains unknown whether yam bean fiber (YB) is also capable of counteracting the deleterious effects of a high-fat diet (HFD) on the small intestine. This present study aimed to investigate the effect of YB supplementation on the glucagon-like peptide 1 (GLP-1) expression, oxidative stress, and histopathological alterations of the small intestine in mice fed an HFD. Twenty-seven mice were assigned equally into three different diet treatment groups: normal diet (ND), HFD, and HFD supplemented with 10% of YB (HFD + YB). After eight weeks, mRNA expression of GLP-1, malondialdehyde (MDA) levels, catalase (CAT) activity in the small intestinal tissue were determined, and the anatomical and histopathological alterations of the intestine were investigated. The results demonstrated that YB supplementation was effective in precluding HFD-indued reduction of GLP-1 mRNA expression in the small intestine. YBF also prevented MDA elevation while ameliorating CAT activity. Furthermore, YBF exerted an ameliorative effect against HFD-induced morphological and histological alterations of the small intestine, particularly in intestinal length, index, and the number of inflammatory cells in the mucosal layer. This study revealed that YB supplementation could effectively prevent the impairment of GLP-1 expression and oxidative stress while ameliorating the histopathological alterations of the small intestine caused by HFD. Hence, YB could be formulated as a supplement to improve the gastrointestinal structure and functions against HFD.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Putra Santoso ^*
0000-0003-4092-6222
Indonesia

Wilka Ramadhia This is me
0009-0003-0250-988X
Indonesia

Resti Rahayu This is me
0000-0002-8882-0593
Indonesia

Rita Maliza This is me
0000-0002-8494-314X
Indonesia

Publication Date

June 28, 2025

Submission Date

May 27, 2023

Acceptance Date

July 27, 2023

Published in Issue

Year 2024 Volume: 28 Number: 5

IZ

https://izlik.org/JA78WZ97CY

Cite

RIS / Bibtex

APA

Santoso, P., Ramadhia, W., Rahayu, R., & Maliza, R. (2025). Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. Journal of Research in Pharmacy, 28(5), 1400-1408. https://izlik.org/JA78WZ97CY

AMA

1.Santoso P, Ramadhia W, Rahayu R, Maliza R. Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. J. Res. Pharm. 2025;28(5):1400-1408. https://izlik.org/JA78WZ97CY

Chicago

Santoso, Putra, Wilka Ramadhia, Resti Rahayu, and Rita Maliza. 2025. “Ameliorative Effect of Yam Bean (Pachyrhizus Erosus L.) Fiber on Glucagon-Like Peptide 1 Expression, Oxidative Stress and Histopathology of the Small ıntestine in Mice Fed High-Fat Diet”. Journal of Research in Pharmacy 28 (5): 1400-1408. https://izlik.org/JA78WZ97CY.

EndNote

Santoso P, Ramadhia W, Rahayu R, Maliza R (July 1, 2025) Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. Journal of Research in Pharmacy 28 5 1400–1408.

IEEE

[1]P. Santoso, W. Ramadhia, R. Rahayu, and R. Maliza, “Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet”, J. Res. Pharm., vol. 28, no. 5, pp. 1400–1408, July 2025, [Online]. Available: https://izlik.org/JA78WZ97CY

ISNAD

Santoso, Putra - Ramadhia, Wilka - Rahayu, Resti - Maliza, Rita. “Ameliorative Effect of Yam Bean (Pachyrhizus Erosus L.) Fiber on Glucagon-Like Peptide 1 Expression, Oxidative Stress and Histopathology of the Small ıntestine in Mice Fed High-Fat Diet”. Journal of Research in Pharmacy 28/5 (July 1, 2025): 1400-1408. https://izlik.org/JA78WZ97CY.

JAMA

1.Santoso P, Ramadhia W, Rahayu R, Maliza R. Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. J. Res. Pharm. 2025;28:1400–1408.

MLA

Santoso, Putra, et al. “Ameliorative Effect of Yam Bean (Pachyrhizus Erosus L.) Fiber on Glucagon-Like Peptide 1 Expression, Oxidative Stress and Histopathology of the Small ıntestine in Mice Fed High-Fat Diet”. Journal of Research in Pharmacy, vol. 28, no. 5, July 2025, pp. 1400-8, https://izlik.org/JA78WZ97CY.

Vancouver

1.Putra Santoso, Wilka Ramadhia, Resti Rahayu, Rita Maliza. Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet. J. Res. Pharm. [Internet]. 2025 Jul. 1;28(5):1400-8. Available from: https://izlik.org/JA78WZ97CY