Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

Beyza Ayazgök; Zekiye Tuba Tüylü Küçükkılınç

Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide

Abstract

Bisphenol A (BPA, 2,2-bis(4-hydroxyphenyl)propane)polycarbonate is an industrial component frequently used as an additive in the construction of epoxy resin and other non-polymer plastics. In this study cytotoxicity of low dose BPA (0.01 μM-100 μM ) and NO levels in SH-SY5Y cells were assessed together to determine whether there is a correlation between of them. MTT Cytotoxicity assays were performed by applying BPA at increasing doses (0.01 μM-100 μM) for 8, 24, 72 hours. Measurements to determine the nitric oxide (NO) levels of the cells, were made using the Griess method at the same time intervals and at the equal doses with BPA. It is found that percentage viability values in SH-SY5Y cells treated with 100 µM BPA for 8 hours, is reduced statistically significant compared to control groups (p<0.001). A significant correlation was found between cytotoxicity results and NO levels of cells treated BPA (0.01 μM-100 μM) for 8 and 72 hours ( p<0.01, p<0.001 respectively). Neurotoxic effects of low dose BPA exposure on SH-SY5Y cells may be explained with increase in the levels of NO as a result from different parameters. One of these parameters could be a disorder in the synthesis of nNOS on different stages such as transcription and translation.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Beyza Ayazgök This is me
Türkiye

Zekiye Tuba Tüylü Küçükkılınç ^*
Türkiye

Publication Date

June 27, 2025

Submission Date

October 16, 2018

Acceptance Date

December 19, 2018

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA96DS96HH

Cite

RIS / Bibtex

APA

Ayazgök, B., & Tüylü Küçükkılınç, Z. T. (2025). Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide. Journal of Research in Pharmacy, 23(3), 354-359. https://izlik.org/JA96DS96HH

AMA

1.Ayazgök B, Tüylü Küçükkılınç ZT. Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide. J. Res. Pharm. 2025;23(3):354-359. https://izlik.org/JA96DS96HH

Chicago

Ayazgök, Beyza, and Zekiye Tuba Tüylü Küçükkılınç. 2025. “Neurotoxic Effects of Bisphenol A on SH-SY5Y Neuroblastoma Cells via Nitric Oxide”. Journal of Research in Pharmacy 23 (3): 354-59. https://izlik.org/JA96DS96HH.

EndNote

Ayazgök B, Tüylü Küçükkılınç ZT (June 1, 2025) Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide. Journal of Research in Pharmacy 23 3 354–359.

IEEE

[1]B. Ayazgök and Z. T. Tüylü Küçükkılınç, “Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide”, J. Res. Pharm., vol. 23, no. 3, pp. 354–359, June 2025, [Online]. Available: https://izlik.org/JA96DS96HH

ISNAD

Ayazgök, Beyza - Tüylü Küçükkılınç, Zekiye Tuba. “Neurotoxic Effects of Bisphenol A on SH-SY5Y Neuroblastoma Cells via Nitric Oxide”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 354-359. https://izlik.org/JA96DS96HH.

JAMA

1.Ayazgök B, Tüylü Küçükkılınç ZT. Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide. J. Res. Pharm. 2025;23:354–359.

MLA

Ayazgök, Beyza, and Zekiye Tuba Tüylü Küçükkılınç. “Neurotoxic Effects of Bisphenol A on SH-SY5Y Neuroblastoma Cells via Nitric Oxide”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 354-9, https://izlik.org/JA96DS96HH.

Vancouver

1.Beyza Ayazgök, Zekiye Tuba Tüylü Küçükkılınç. Neurotoxic effects of bisphenol A on SH-SY5Y neuroblastoma cells via nitric oxide. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):354-9. Available from: https://izlik.org/JA96DS96HH