A novel fluorescent-based HPLC method for determination of urolithins

Emine Vildan Burgaz; Bahareh Noshadi; Hayrettin Ozan Gulcan

A novel fluorescent-based HPLC method for determination of urolithins

Abstract

Urolithins are the bioavailable metabolites of ellagitannins, abundantly available in dietaries routinely consumed. The number of research studies growingly enlarges on the beneficial effects of these natural chemicals on human health. From this perspective, one aspect of scientific interest in urolithins covers the development of techniques for their qualitative and quantitative analysis in diverse samples. UV-based and tandem-mass spectroscopic analyses have been the major ones used and applied so far. However, the fluorescent characteristics of these molecules have not received sufficient attention. Within the scope of this research study, major urolithins (Urolithin A and B) concomitant to their methyl ether metabolites have been synthesized and their quantitative analysis has been investigated via an HPLC system connected to fluorescence detection. The results displayed the superior and comparable characteristics of the methodology developed against the previous techniques.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Emine Vildan Burgaz This is me
0000-0003-3245-5934
Cyprus

Bahareh Noshadi This is me
0000-0002-2650-0148
Cyprus

Hayrettin Ozan Gulcan ^* This is me
0000-0002-9503-5841
Cyprus

Publication Date

June 28, 2025

Submission Date

March 6, 2023

Acceptance Date

May 6, 2023

Published in Issue

Year 2023 Volume: 27 Number: 5

IZ

https://izlik.org/JA32NG44UT

Cite

RIS / Bibtex

APA

Burgaz, E. V., Noshadi, B., & Gulcan, H. O. (2025). A novel fluorescent-based HPLC method for determination of urolithins. Journal of Research in Pharmacy, 27(5), 2007-2016. https://izlik.org/JA32NG44UT

AMA

1.Burgaz EV, Noshadi B, Gulcan HO. A novel fluorescent-based HPLC method for determination of urolithins. J. Res. Pharm. 2025;27(5):2007-2016. https://izlik.org/JA32NG44UT

Chicago

Burgaz, Emine Vildan, Bahareh Noshadi, and Hayrettin Ozan Gulcan. 2025. “A Novel Fluorescent-Based HPLC Method for Determination of Urolithins”. Journal of Research in Pharmacy 27 (5): 2007-16. https://izlik.org/JA32NG44UT.

EndNote

Burgaz EV, Noshadi B, Gulcan HO (July 1, 2025) A novel fluorescent-based HPLC method for determination of urolithins. Journal of Research in Pharmacy 27 5 2007–2016.

IEEE

[1]E. V. Burgaz, B. Noshadi, and H. O. Gulcan, “A novel fluorescent-based HPLC method for determination of urolithins”, J. Res. Pharm., vol. 27, no. 5, pp. 2007–2016, July 2025, [Online]. Available: https://izlik.org/JA32NG44UT

ISNAD

Burgaz, Emine Vildan - Noshadi, Bahareh - Gulcan, Hayrettin Ozan. “A Novel Fluorescent-Based HPLC Method for Determination of Urolithins”. Journal of Research in Pharmacy 27/5 (July 1, 2025): 2007-2016. https://izlik.org/JA32NG44UT.

JAMA

1.Burgaz EV, Noshadi B, Gulcan HO. A novel fluorescent-based HPLC method for determination of urolithins. J. Res. Pharm. 2025;27:2007–2016.

MLA

Burgaz, Emine Vildan, et al. “A Novel Fluorescent-Based HPLC Method for Determination of Urolithins”. Journal of Research in Pharmacy, vol. 27, no. 5, July 2025, pp. 2007-16, https://izlik.org/JA32NG44UT.

Vancouver

1.Emine Vildan Burgaz, Bahareh Noshadi, Hayrettin Ozan Gulcan. A novel fluorescent-based HPLC method for determination of urolithins. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(5):2007-16. Available from: https://izlik.org/JA32NG44UT