Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans

Isnaeni Isnaeni; Agustin Maulidina; Idha Kusumawati; Erni Maduratna Setyawatie

Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans

Abstract

The growth inhibitory activity of Lactobacillus plantarum ATCC 8014 (Lp) fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutants has been studied. The preparation is potential developed as healthy food due to its nutritional value as well as its beneficial activities. The well agar diffusion method was applied for the antibacterial assay on nutrient agar media and local strain of Streptococcus mutants was used as a bacterial test. This species often causes infectious in the teeth. Effectiveness of the fermented milk and its combination with aqueous extract preparations against the bacterial test were assessed by minimum inhibitory concentration (MIC) for the potency indication. The aqueous extract of Moringa oleifera leaves was prepared as infuse like according to community daily consumption. Concentration of the extract used in the formulation was higher than the MIC. The result showed that the MIC of the aqueous extract of Moringa oleifera leaves and the L. plantarum fermented milk were 20% and 35% with growth inhibition zone diameter of 11.70 ± 0.28 mm and 12.02 ± 0.83 mm, respectively. The combination of fermented milk and aqueous extract was prepared in various ratio concentration (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, and 9:1). The optimum ratio of the combination was 2:8 with growth inhibition zone diameter of 16.53 ± 0.32 mm and the MIC value at optimum ratio was 45% with inhibition zone diameter of 11.90 ± 0.86 mm.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy, Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Isnaeni Isnaeni ^* This is me
Indonesia

Agustin Maulidina This is me
Indonesia

Idha Kusumawati This is me
Indonesia

Erni Maduratna Setyawatie This is me
Indonesia

Publication Date

June 27, 2025

Submission Date

January 23, 2019

Acceptance Date

May 1, 2019

Published in Issue

Year 2019 Volume: 23 Number: 4

IZ

https://izlik.org/JA82ER55FP

Cite

RIS / Bibtex

APA

Isnaeni, I., Maulidina, A., Kusumawati, I., & Setyawatie, E. M. (2025). Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans. Journal of Research in Pharmacy, 23(4), 701-710. https://izlik.org/JA82ER55FP

AMA

1.Isnaeni I, Maulidina A, Kusumawati I, Setyawatie EM. Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans. J. Res. Pharm. 2025;23(4):701-710. https://izlik.org/JA82ER55FP

Chicago

Isnaeni, Isnaeni, Agustin Maulidina, Idha Kusumawati, and Erni Maduratna Setyawatie. 2025. “Inhibitory Activity of Lactobacillus Plantarum ATCC 8014 Fermented Milk Combined With Aqueous Extract of Moringa Oleifera Leaves Against Streptococcus Mutans”. Journal of Research in Pharmacy 23 (4): 701-10. https://izlik.org/JA82ER55FP.

EndNote

Isnaeni I, Maulidina A, Kusumawati I, Setyawatie EM (June 1, 2025) Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans. Journal of Research in Pharmacy 23 4 701–710.

IEEE

[1]I. Isnaeni, A. Maulidina, I. Kusumawati, and E. M. Setyawatie, “Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans”, J. Res. Pharm., vol. 23, no. 4, pp. 701–710, June 2025, [Online]. Available: https://izlik.org/JA82ER55FP

ISNAD

Isnaeni, Isnaeni - Maulidina, Agustin - Kusumawati, Idha - Setyawatie, Erni Maduratna. “Inhibitory Activity of Lactobacillus Plantarum ATCC 8014 Fermented Milk Combined With Aqueous Extract of Moringa Oleifera Leaves Against Streptococcus Mutans”. Journal of Research in Pharmacy 23/4 (June 1, 2025): 701-710. https://izlik.org/JA82ER55FP.

JAMA

1.Isnaeni I, Maulidina A, Kusumawati I, Setyawatie EM. Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans. J. Res. Pharm. 2025;23:701–710.

MLA

Isnaeni, Isnaeni, et al. “Inhibitory Activity of Lactobacillus Plantarum ATCC 8014 Fermented Milk Combined With Aqueous Extract of Moringa Oleifera Leaves Against Streptococcus Mutans”. Journal of Research in Pharmacy, vol. 23, no. 4, June 2025, pp. 701-10, https://izlik.org/JA82ER55FP.

Vancouver

1.Isnaeni Isnaeni, Agustin Maulidina, Idha Kusumawati, Erni Maduratna Setyawatie. Inhibitory activity of Lactobacillus plantarum ATCC 8014 fermented milk combined with aqueous extract of Moringa oleifera leaves against Streptococcus mutans. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(4):701-10. Available from: https://izlik.org/JA82ER55FP