In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract

Mehmet Evren Okur; Nihal Karakaş; Ayşe Esra Karadağ; Rabia Yılmaz Öztürk; Fatih Demirci

In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract

Abstract

Marrubium vulgare L. (Lamiaceae) is a herbal drug used for centuries for many diseases. In this present study the plant material was acquired from commercial sources in pharma grade (PhEur 8.0) quality. The methanol extract of the aerial parts was evaluated for its in vitro cytotoxic activity by measuring the percentage of viable cells (U87 LN229 and T98G glioblastoma multiforme cell lines) using a luminescence system, and the antioxidant activities by ABTS and DPPH radical scavenging spectrophotometrically. As a result, the methanol extract of M. vulgare showed 48.97 ± 0.82 mg of GA/g corresponding to the total phenolic amounts, and moderate antioxidant activity (1.33 and 2.08) by ABTS• and DPPH• assays. To the best of knowledge, after evaluation of the cytotoxicity on M. vulgare treated U87 (IC50:270.3 μM), LN229 (IC50:343.9 μM) and T98G (336.6 μM) glioblastoma multiforme (GBM) cell lines, significant cytotoxic activities with 69.9% (p= 0.0081) and 71% cell viability (p= 0.0028) was observed in M. vulgare treated U87 and LN229 GBM cell lines, respectively. Overall, further in vitro and in vivo bioactivity studies based on phytochemistry on various M. vulgare preparations are worthwhile in order to discover bioactive secondary metabolites.

Keywords

References

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Details

Primary Language

English

Subjects

Clinical Pharmacy and Pharmacy Practice

Journal Section

Research Article

Authors

Mehmet Evren Okur ^*
Türkiye

Nihal Karakaş
Türkiye

Ayşe Esra Karadağ
Türkiye

Rabia Yılmaz Öztürk
Türkiye

Fatih Demirci
Türkiye

Publication Date

June 27, 2025

Submission Date

January 10, 2019

Acceptance Date

May 22, 2019

Published in Issue

Year 2019 Volume: 23 Number: 4

IZ

https://izlik.org/JA89MP39RG

Cite

RIS / Bibtex

APA

Okur, M. E., Karakaş, N., Karadağ, A. E., Yılmaz Öztürk, R., & Demirci, F. (2025). In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. Journal of Research in Pharmacy, 23(4), 711-718. https://izlik.org/JA89MP39RG

AMA

1.Okur ME, Karakaş N, Karadağ AE, Yılmaz Öztürk R, Demirci F. In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. J. Res. Pharm. 2025;23(4):711-718. https://izlik.org/JA89MP39RG

Chicago

Okur, Mehmet Evren, Nihal Karakaş, Ayşe Esra Karadağ, Rabia Yılmaz Öztürk, and Fatih Demirci. 2025. “In Vitro Cytotoxicity Evaluation of Marrubium Vulgare L. Methanol Extract”. Journal of Research in Pharmacy 23 (4): 711-18. https://izlik.org/JA89MP39RG.

EndNote

Okur ME, Karakaş N, Karadağ AE, Yılmaz Öztürk R, Demirci F (June 1, 2025) In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. Journal of Research in Pharmacy 23 4 711–718.

IEEE

[1]M. E. Okur, N. Karakaş, A. E. Karadağ, R. Yılmaz Öztürk, and F. Demirci, “In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract”, J. Res. Pharm., vol. 23, no. 4, pp. 711–718, June 2025, [Online]. Available: https://izlik.org/JA89MP39RG

ISNAD

Okur, Mehmet Evren - Karakaş, Nihal - Karadağ, Ayşe Esra - Yılmaz Öztürk, Rabia - Demirci, Fatih. “In Vitro Cytotoxicity Evaluation of Marrubium Vulgare L. Methanol Extract”. Journal of Research in Pharmacy 23/4 (June 1, 2025): 711-718. https://izlik.org/JA89MP39RG.

JAMA

1.Okur ME, Karakaş N, Karadağ AE, Yılmaz Öztürk R, Demirci F. In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. J. Res. Pharm. 2025;23:711–718.

MLA

Okur, Mehmet Evren, et al. “In Vitro Cytotoxicity Evaluation of Marrubium Vulgare L. Methanol Extract”. Journal of Research in Pharmacy, vol. 23, no. 4, June 2025, pp. 711-8, https://izlik.org/JA89MP39RG.

Vancouver

1.Mehmet Evren Okur, Nihal Karakaş, Ayşe Esra Karadağ, Rabia Yılmaz Öztürk, Fatih Demirci. In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(4):711-8. Available from: https://izlik.org/JA89MP39RG