Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis

Tolga Akkoç; Burak Aksu

Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis

Abstract

The aim is to investigate how Umbilical Cord-Derived Mesenchymal Stem Cells conditioned medium (UC-MSC-CM) can promote wound healing and epithelial barrier function. UC-MSCs were differentiated into osteocytes, adipocytes, and chondrocytes to determine their multipotency. Flow cytometric analyses confirmed positive surface markers (CD90, CD73, CD105) and the absence of negative markers (CD34, CD11b, CD19, HLA_DR). We prepared conditioned medium (CM) from these cells. UC-MSC CM was tested in in vitro wound healing experiments using scratch assays and transepithelial electrical resistance measurements at 6th, 12th, and 24th hours to assess its regenerative effects. UC-MSCs were found to be multipotent in the characterization and differentiation analysis, demonstrating their potential for use in regenerative medicine. In our wound model design, UC-MSC-CM significantly accelerated the healing process 75% and 92.7% at 12 and 24 hours respectively compared to the control group. According to TEER analyzes epithelial barrier resistance showed 200 Ω.cm2 with UC-MSC-CM healing process at the 12th and 24th hours while the control group showed 145 Ω.cm2 and 185 Ω.cm2 at the same time points, respectively. The study demonstrates the potential of UC-MSCs in repairing epithelial barriers and wound injuries. The UC-MSC-CM accelerates wound closure as the healing process advances and enhances barrier integrity and permeability. These findings highlight UC-MSC-CM as a cell-free therapy for regenerative medicine and wound healing. Further research should focus on understanding the underlying mechanisms and optimizing its therapeutic applications to revolutionize wound management and treatment.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Tolga Akkoç ^* This is me
0000-0002-4849-1473
Türkiye

Burak Aksu
0000-0002-3439-9158
Türkiye

Publication Date

June 28, 2025

Submission Date

August 3, 2023

Acceptance Date

August 18, 2023

Published in Issue

Year 2023 Volume: 27 Number: 5

IZ

https://izlik.org/JA56CU23PM

Cite

RIS / Bibtex

APA

Akkoç, T., & Aksu, B. (2025). Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis. Journal of Research in Pharmacy, 27(5), 2135-2144. https://izlik.org/JA56CU23PM

AMA

1.Akkoç T, Aksu B. Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis. J. Res. Pharm. 2025;27(5):2135-2144. https://izlik.org/JA56CU23PM

Chicago

Akkoç, Tolga, and Burak Aksu. 2025. “Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis”. Journal of Research in Pharmacy 27 (5): 2135-44. https://izlik.org/JA56CU23PM.

EndNote

Akkoç T, Aksu B (July 1, 2025) Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis. Journal of Research in Pharmacy 27 5 2135–2144.

IEEE

[1]T. Akkoç and B. Aksu, “Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis”, J. Res. Pharm., vol. 27, no. 5, pp. 2135–2144, July 2025, [Online]. Available: https://izlik.org/JA56CU23PM

ISNAD

Akkoç, Tolga - Aksu, Burak. “Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis”. Journal of Research in Pharmacy 27/5 (July 1, 2025): 2135-2144. https://izlik.org/JA56CU23PM.

JAMA

1.Akkoç T, Aksu B. Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis. J. Res. Pharm. 2025;27:2135–2144.

MLA

Akkoç, Tolga, and Burak Aksu. “Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis”. Journal of Research in Pharmacy, vol. 27, no. 5, July 2025, pp. 2135-44, https://izlik.org/JA56CU23PM.

Vancouver

1.Tolga Akkoç, Burak Aksu. Investigation of The Efficacy of Mesenchymal Stem CellDerived Conditioned Medium on Wound Healing Through Scratch Test and Teer Analysis. J. Res. Pharm. [Internet]. 2025 Jul. 1;27(5):2135-44. Available from: https://izlik.org/JA56CU23PM