The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent

Achmad Fuad Hafid; Hilkatul Ilmi; Hanifah Khairun Nisa; Lidya Tumewu; Myrna Adianti; Tutik Sri Wahyuni; Suciati Suciati; Aty Widyawaruyanti

The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent

Abstract

The combination of Luvunga sarmentosa and Eurycoma longifolia is widely prescribed in traditional medicine by the local Dayak tribe in Central Kalimantan, Indonesia. The study aimed to evaluate the effect of antioxidants and analgesics from the combination of hydro-alcoholic extract of L. sarmentosa and E. longifolia stem. The phytochemical investigation was performed using an established method described in the literature. The total phenolic content was determined by the Folin Ciocalteu, whereas the flavonoid was determined by aluminum chloride. The in vitro antioxidant activity was determined using DPPH free radical scavenging assay. The analgesic effect was determined by acetic acid-induced writhing and hot plate tests in male mice. The result shows that combination extract (CE, IC50 162.5±0.21 µg/mL) had higher antioxidant activity than L. sarmentosa extract (LSE, IC50 200.20±0.23 µg/mL) and E. longifolia extract (ELE, IC50 293.45±0.32 µg/mL). All extracts showed significant dose-dependent analgesic activity against acetic acid-induced writhing and hot plate pain models. A higher dose of CE, LSE, and ELE (550 mg/kg) produced significant inhibition of writhing by 67.60±2.76, 56.45±2.34, and 68.64±1.65%, respectively. The maximum possible analgesia of CE, LSE, and ELE in the hot plate test at 550 mg/kg BW dose was 25.32%, 16.04%, and 26.72%. CE has higher analgesic activity than LSE and ELE. The extracts are more effective for peripheral than central analgesia. Its mechanism may be related to inhibiting the inflammatory mediator's release. The high antioxidant and analgesic activity at CE is relevant to the higher total flavones and phenols compared to LSE and ELE.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Sciences

Journal Section

Research Article

Authors

Achmad Fuad Hafid ^* This is me
0000-0002-0570-7975
Indonesia

Hilkatul Ilmi This is me
0000-0002-3844-8877
Indonesia

Hanifah Khairun Nisa This is me
0000-0002-7010-7036
Indonesia

Lidya Tumewu This is me
0000-0003-3132-8909
Indonesia

Myrna Adianti This is me
0000-0002-9262-9847
Indonesia

Tutik Sri Wahyuni This is me
0000-0003-2049-7774
Indonesia

Suciati Suciati This is me
0000-0003-2436-4119
Indonesia

Aty Widyawaruyanti This is me
0000-0003-4639-1599
Indonesia

Publication Date

June 27, 2025

Submission Date

July 5, 2022

Acceptance Date

December 1, 2022

Published in Issue

Year 2023 Volume: 27 Number: 2

IZ

https://izlik.org/JA69AE23YY

Cite

RIS / Bibtex

APA

Hafid, A. F., Ilmi, H., Nisa, H. K., Tumewu, L., Adianti, M., Wahyuni, T. S., Suciati, S., & Widyawaruyanti, A. (2025). The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent. Journal of Research in Pharmacy, 27(2), 642-651. https://izlik.org/JA69AE23YY

AMA

1.Hafid AF, Ilmi H, Nisa HK, et al. The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent. J. Res. Pharm. 2025;27(2):642-651. https://izlik.org/JA69AE23YY

Chicago

Hafid, Achmad Fuad, Hilkatul Ilmi, Hanifah Khairun Nisa, et al. 2025. “The Combination of Luvunga Sarmentosa (BI.) and Eurycoma Longifolia Jackhydro-Alcoholic Extract As a Source of Antioxidant and Analgesic Agent”. Journal of Research in Pharmacy 27 (2): 642-51. https://izlik.org/JA69AE23YY.

EndNote

Hafid AF, Ilmi H, Nisa HK, Tumewu L, Adianti M, Wahyuni TS, Suciati S, Widyawaruyanti A (June 1, 2025) The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent. Journal of Research in Pharmacy 27 2 642–651.

IEEE

[1]A. F. Hafid et al., “The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent”, J. Res. Pharm., vol. 27, no. 2, pp. 642–651, June 2025, [Online]. Available: https://izlik.org/JA69AE23YY

ISNAD

Hafid, Achmad Fuad - Ilmi, Hilkatul - Nisa, Hanifah Khairun - Tumewu, Lidya - Adianti, Myrna - Wahyuni, Tutik Sri - Suciati, Suciati - Widyawaruyanti, Aty. “The Combination of Luvunga Sarmentosa (BI.) and Eurycoma Longifolia Jackhydro-Alcoholic Extract As a Source of Antioxidant and Analgesic Agent”. Journal of Research in Pharmacy 27/2 (June 1, 2025): 642-651. https://izlik.org/JA69AE23YY.

JAMA

1.Hafid AF, Ilmi H, Nisa HK, Tumewu L, Adianti M, Wahyuni TS, Suciati S, Widyawaruyanti A. The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent. J. Res. Pharm. 2025;27:642–651.

MLA

Hafid, Achmad Fuad, et al. “The Combination of Luvunga Sarmentosa (BI.) and Eurycoma Longifolia Jackhydro-Alcoholic Extract As a Source of Antioxidant and Analgesic Agent”. Journal of Research in Pharmacy, vol. 27, no. 2, June 2025, pp. 642-51, https://izlik.org/JA69AE23YY.

Vancouver

1.Achmad Fuad Hafid, Hilkatul Ilmi, Hanifah Khairun Nisa, Lidya Tumewu, Myrna Adianti, Tutik Sri Wahyuni, Suciati Suciati, Aty Widyawaruyanti. The combination of Luvunga sarmentosa (BI.) and Eurycoma longifolia Jackhydro-alcoholic extract as a source of antioxidant and analgesic agent. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(2):642-51. Available from: https://izlik.org/JA69AE23YY