Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation

Jasmine Chaudhary; Akash Jain; Vipin Saini

Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation

Abstract

Paracetamol with Promethazine HCl is most popular over the counter drug which is used as antipyretic and antiemetic. A novel, simple, precise, less time consuming, economical and accurate reverse phase HPLC method has been developed for their estimation using water, methanol and acetic acid in 79:20:1 v/v/v ratio as eluting phase on Kromasil Silica column keeping flow rate at 1ml/min and detection at 249 nm. Both drugs observed linearity between 10-50μg/ml and successfully resolved within 6 minutes (3.565 and 5.641 minutes for paracetamol and promethazine respectively) with percent recovery between 98-101%. Tailing factor is within the range and number of theoretical plates is more than 2500. Method was validated as per ICH guidelines and the results indicate that these drugs could be quantified simultaneously without excipient interference and thus suitable for routine analysis of drugs in combination.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Jasmine Chaudhary ^*
India

Akash Jain This is me
India

Vipin Saini This is me
India

Publication Date

June 27, 2025

Submission Date

October 4, 2018

Acceptance Date

February 7, 2019

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA67PN69KK

Cite

RIS / Bibtex

APA

Chaudhary, J., Jain, A., & Saini, V. (2025). Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation. Journal of Research in Pharmacy, 23(3), 476-483. https://izlik.org/JA67PN69KK

AMA

1.Chaudhary J, Jain A, Saini V. Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation. J. Res. Pharm. 2025;23(3):476-483. https://izlik.org/JA67PN69KK

Chicago

Chaudhary, Jasmine, Akash Jain, and Vipin Saini. 2025. “Novel RP-HPLC Method for Estimation of Paracetamol and Promethazine Simultaneously in Syrup Formulation”. Journal of Research in Pharmacy 23 (3): 476-83. https://izlik.org/JA67PN69KK.

EndNote

Chaudhary J, Jain A, Saini V (June 1, 2025) Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation. Journal of Research in Pharmacy 23 3 476–483.

IEEE

[1]J. Chaudhary, A. Jain, and V. Saini, “Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation”, J. Res. Pharm., vol. 23, no. 3, pp. 476–483, June 2025, [Online]. Available: https://izlik.org/JA67PN69KK

ISNAD

Chaudhary, Jasmine - Jain, Akash - Saini, Vipin. “Novel RP-HPLC Method for Estimation of Paracetamol and Promethazine Simultaneously in Syrup Formulation”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 476-483. https://izlik.org/JA67PN69KK.

JAMA

1.Chaudhary J, Jain A, Saini V. Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation. J. Res. Pharm. 2025;23:476–483.

MLA

Chaudhary, Jasmine, et al. “Novel RP-HPLC Method for Estimation of Paracetamol and Promethazine Simultaneously in Syrup Formulation”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 476-83, https://izlik.org/JA67PN69KK.

Vancouver

1.Jasmine Chaudhary, Akash Jain, Vipin Saini. Novel RP-HPLC method for estimation of paracetamol and promethazine simultaneously in syrup formulation. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):476-83. Available from: https://izlik.org/JA67PN69KK