Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia

Oluwaseun Ruth Alara; Abdurahman Nour

Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia

Abstract

Diabetes has been characterized by transmuted lipids, hyperglycemia, proteins, and carbohydrates metabolism that can affect the quality of life. Although there are quite several synthetic drugs used in managing this disease, however, due to their side effects, the use of natural sources is imperative. This study focused on the in-vitro inhibitory activity of Vernonia amygdalina leaf grown in Malaysia using both α-amylase and α-glucosidase assays. The quantities of mineral elements in the plant extracts through microwave-assisted and Soxhlet extraction methods were as well examined through inductively coupled plasma mass spectrometry (ICP-MS) analysis. The extract of V. amygdalina leaf showed the highest dose-dependent effect of 97.28 and 98.08% of inhibition at 1.0 mg/mL having IC50 values of 0.51 mg/mL and 0.49 mg/mL against α-amylase and α-glucosidase enzymes, respectively. The inhibitory effects of the extract were higher than acarbose (a standard drug). Moreover, the results showed that higher quantities of mineral elements (potassium, magnesium, and calcium) and a lesser quantity of chromium in the extracts through microwave-assisted extraction (MAE) indicate that the technique possesses a higher capacity in obtaining higher mineral nutrients over Soxhlet extraction and more anti-diabetic activity of the extract. Thus, the extract exhibited an effective anti-diabetic activity which implies that it potentially serves as a natural source for managing diabetes.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry

Journal Section

Research Article

Authors

Oluwaseun Ruth Alara ^* This is me
Malaysia

Abdurahman Nour
Malaysia

Publication Date

June 27, 2025

Submission Date

December 24, 2018

Acceptance Date

February 10, 2019

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA76ZJ44XS

Cite

RIS / Bibtex

APA

Alara, O. R., & Nour, A. (2025). Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia. Journal of Research in Pharmacy, 23(3), 514-521. https://izlik.org/JA76ZJ44XS

AMA

1.Alara OR, Nour A. Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia. J. Res. Pharm. 2025;23(3):514-521. https://izlik.org/JA76ZJ44XS

Chicago

Alara, Oluwaseun Ruth, and Abdurahman Nour. 2025. “Anti-Diabetic Activity and Mineral Elements Evaluation of Vernonia Amygdalina Leaves Obtained from Malaysia”. Journal of Research in Pharmacy 23 (3): 514-21. https://izlik.org/JA76ZJ44XS.

EndNote

Alara OR, Nour A (June 1, 2025) Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia. Journal of Research in Pharmacy 23 3 514–521.

IEEE

[1]O. R. Alara and A. Nour, “Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia”, J. Res. Pharm., vol. 23, no. 3, pp. 514–521, June 2025, [Online]. Available: https://izlik.org/JA76ZJ44XS

ISNAD

Alara, Oluwaseun Ruth - Nour, Abdurahman. “Anti-Diabetic Activity and Mineral Elements Evaluation of Vernonia Amygdalina Leaves Obtained from Malaysia”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 514-521. https://izlik.org/JA76ZJ44XS.

JAMA

1.Alara OR, Nour A. Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia. J. Res. Pharm. 2025;23:514–521.

MLA

Alara, Oluwaseun Ruth, and Abdurahman Nour. “Anti-Diabetic Activity and Mineral Elements Evaluation of Vernonia Amygdalina Leaves Obtained from Malaysia”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 514-21, https://izlik.org/JA76ZJ44XS.

Vancouver

1.Oluwaseun Ruth Alara, Abdurahman Nour. Anti-diabetic activity and mineral elements evaluation of Vernonia amygdalina leaves obtained from Malaysia. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):514-21. Available from: https://izlik.org/JA76ZJ44XS