Colorimetric analysis of phenols and flavanoids in pomegranate plant extract

Sujiya Balla; Nagasen Dasari; Sharon Priyanka Palina; Sathis Kumar Dinakaran; Lavanya Pulidindi

Colorimetric analysis of phenols and flavanoids in pomegranate plant extract

Abstract

Punica granatum (Pomegranate) is widely accessible and used by natural health professionals for a range of medicinal purposes. The purpose of the current study to use a derivatization method in conjunction with FT-IR, colorimetry, and UV-Visible spectroscopy to determine the total phenolic and flavonoid content of various pomegranate sections. Pomegranate is used to treat ulcers, diarrhoea, Male infertility and so on. Currently, pomegranate is marketed in different novel drug formulations such as nanoparticles for local mucosal drug delivery, niosomes, hydrogels for chronic ınflammation, microemulsion for skin care and analytical techniques using HPLC, GC and GC-MS, LC-MS for carrying out the various quantitative and qualitative analyses are some examples of these developments. In this study, a part of pomegranate was extracted and evaluated by using Microscopical and Macroscopical evaluation, Fluorescence Analysis, FT-IR studies using derivatisation method and finally quantified the phenol and flavonoid content using UV Visible Spectroscopy. Results of the study revealed that the pomegranate extract gives data about the linearity, precision, accuracy of peel and bark ethanolic and aqueous extract gives linearity 1-5µg/ml, Regression 0.998, LOD 0.691µg/ml, LOQ 1.32µg/ml. Through the appropriate selection of Solvents, we successfully developed a stable and effective method for the determination and estimation of phenol and flavonoid content by derivatization and FTIR studies. Method was developed, optimized and validated with its parameters.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Sujiya Balla ^* This is me
0009-0009-5181-7313
India

Nagasen Dasari This is me
0000-0002-8305-7277
India

Sharon Priyanka Palina This is me
0009-0008-0692-1990
India

Sathis Kumar Dinakaran This is me
0000-0002-4163-4785
India

Lavanya Pulidindi This is me
0009-0004-4731-8419
India

Publication Date

June 28, 2025

Submission Date

October 22, 2023

Acceptance Date

January 18, 2024

Published in Issue

Year 2024 Volume: 28 Number: 5

IZ

https://izlik.org/JA23EK24JD

Cite

RIS / Bibtex

APA

Balla, S., Dasari, N., Palina, S. P., Dinakaran, S. K., & Pulidindi, L. (2025). Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. Journal of Research in Pharmacy, 28(5), 1720-1741. https://izlik.org/JA23EK24JD

AMA

1.Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L. Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. J. Res. Pharm. 2025;28(5):1720-1741. https://izlik.org/JA23EK24JD

Chicago

Balla, Sujiya, Nagasen Dasari, Sharon Priyanka Palina, Sathis Kumar Dinakaran, and Lavanya Pulidindi. 2025. “Colorimetric Analysis of Phenols and Flavanoids in Pomegranate Plant Extract”. Journal of Research in Pharmacy 28 (5): 1720-41. https://izlik.org/JA23EK24JD.

EndNote

Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L (July 1, 2025) Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. Journal of Research in Pharmacy 28 5 1720–1741.

IEEE

[1]S. Balla, N. Dasari, S. P. Palina, S. K. Dinakaran, and L. Pulidindi, “Colorimetric analysis of phenols and flavanoids in pomegranate plant extract”, J. Res. Pharm., vol. 28, no. 5, pp. 1720–1741, July 2025, [Online]. Available: https://izlik.org/JA23EK24JD

ISNAD

Balla, Sujiya - Dasari, Nagasen - Palina, Sharon Priyanka - Dinakaran, Sathis Kumar - Pulidindi, Lavanya. “Colorimetric Analysis of Phenols and Flavanoids in Pomegranate Plant Extract”. Journal of Research in Pharmacy 28/5 (July 1, 2025): 1720-1741. https://izlik.org/JA23EK24JD.

JAMA

1.Balla S, Dasari N, Palina SP, Dinakaran SK, Pulidindi L. Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. J. Res. Pharm. 2025;28:1720–1741.

MLA

Balla, Sujiya, et al. “Colorimetric Analysis of Phenols and Flavanoids in Pomegranate Plant Extract”. Journal of Research in Pharmacy, vol. 28, no. 5, July 2025, pp. 1720-41, https://izlik.org/JA23EK24JD.

Vancouver

1.Sujiya Balla, Nagasen Dasari, Sharon Priyanka Palina, Sathis Kumar Dinakaran, Lavanya Pulidindi. Colorimetric analysis of phenols and flavanoids in pomegranate plant extract. J. Res. Pharm. [Internet]. 2025 Jul. 1;28(5):1720-41. Available from: https://izlik.org/JA23EK24JD