Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves

Kamal Ja’afar Muhammad; Shajarahtunnur Jamil; Norazah Basar

Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves

Abstract

Chemical constituents of Scurrula parasitica leaves from Loranthaceae family and some biological activities have been studied. Cold extractions were carried out using n-hexane, ethyl acetate and methanol to obtain the crude extracts. Purification of the extracts led to the isolation of quercetin 1, quercitrin 2, kaempferol 3-O--L-rhamnoside 3, (+)-catechin 4, lupeol 5, lupeol palmitate 6, -sitosterol 7, squalene 8, octacosane 9, octadecane 10 and eicosane 11. The in vitro antioxidant activity of the extracts and isolated compounds were evaluated. Compounds 1, 2, 3 and 4, together with ethyl acetate and methanol extracts exhibited effective antioxidant activities against DPPH, ABTS and FRAP assays, while n-hexane and other compounds were inactive. The ethyl acetate extract gave the highest tyrosinase percent inhibition value of 66.02%, while quercetin gave the best result with tyrosinase percent inhibition value of 79.09%. All extracts and isolated compounds showed weak activity on antimicrobial inhibition assay with the exception of quercetin which exhibited moderate activity against Pseudomonas aeruginosa with MIC and MBC value of 250 g/mL.

Keywords

References

[1] Zakaria M, Mohd, MA. Traditional Malay Medicinal Plants. Fajar Bakti, Kuala Lumpur. 1994.
[2] Adesina SK, Illoh CH, Imoh IJ, Jacobs EI. African mistletoes (Loranthaceae); ethnopharmacology, chemistry and medicinal values: An update. Afr J Tradit Complement Altern Med. 2013; 10(4): 161–170. [CrossRef]
[3] Sher H, Alyemeni MN. Pharmaceutical important plants used in traditional system of Arab medicine for the treatment of livestock ailments in the kingdom of Saudi Arabia. Afr J Biotechnol. 2011; 10(45): 9153-9159. [CrossRef]
[4] Matthes H, Schad F, Buchward D, Schenk G. Endoscopic ultrasound-guided fine needle injection of V. album L. (mistletoe; Helixor M) in the therapy of primary inoperable pancreas cancer; a pilot study. Gastroenterol. 2005: 128(4): 433, T 988.
[5] Lim YC, Rajabalaya R, Shirley HFL, Tennakoon KU, Quang-Vuong L, Idris A, Zulkipli IN, Keasberry N, David SR. Parasitic mistletoes of the genera Scurrula and Viscum: from bench to bedside. Molecules 2016; 21: E1048. [CrossRef]
[6] Puneetha GK, Amruthesh KN. Phytochemical screening and in vitro evaluation of antioxidant activity of various extracts of Scurrula parasitica. Int J Pharm Biol Sci. 2016; 6(1): 77-86.
[7] Xiao YJ, Chen YZ, Chen BH, Chen JH, Lin ZX, Fan YL. Study on cytotoxic activities on human leukemia cell line HL 60 by flavonoids extracts of Scurrula parasitica from four different host trees. China J Chinese Mat Med. 2008; 33(4): 427-432.
[8] Quan-Yu L, Fei W, Lei Z, Jie-Ming X, Lia P, Yong-Hong ZA. Hydroxylated lupeol-based triterpenoid ester isolated from the Scurrula parasitica Parasitic on Nerium indicum. Helv Chim Acta. 2015; 98: 627-632. [CrossRef]

[9] Quan-yu L, Fei W, Yong-Hong Z, Feng NI. Chemical constituents of Scurrula paracitica. China J Chinese Mat Med. 2016; 41(21): 3956-3561.
[10] Muhammad KJ, Shajarahtunnur J, Norazah B. Antioxidant activity of leaf extracts of Globimetula braunii (Engler) van Tiegh Parasitizing on Piliostigma thonningii and Parkia biglobosa. J Teknol. 2017; 79(5): 43–47.
[11] Muhammad KJ, Shajarahtunnur J, Norazah B, Mohd BB, Satyajit DS, Keith JF, Mathias OS. Lactones and flavonoids from the leaves of Globimetula braunii. Nat Prod Comm. 2017; 12(9): 1455-1458.
[12] Suganya T, Fumio I, Siriporn O. Antioxidant active principle isolated from Psidium guajava grown in Thailand. Sci Pharm. 2007; 75: 179-193. [CrossRef]
[13] Hasan SM, Ahmed IM, Mondal S, Uddin SJ, Masud MM, Sadhu SK, Ishibashi M. Antioxidant, antinociceptive activity and general toxicity study of Dendrophthoe falcata and isolation of quercitrin as the major component. Orient Pharm Exp Med. 2006; 6(4): 355-360.
[14] Lee SY, Young-Jin S, Shin MS, Cho YJ, Lee J. Antibacterial effects of afzelin isolated from Cornus macrophylla on Pseudomonas aeruginosa, A leading cause of illness in immunocompromised individuals. Molecules 2014; 19: 3173-3180. [CrossRef]
[15] Lin J, LinY. Flavonoids from the leaves of Loranthus kaoi (Chao). Kiu. J Food Drug Anal. 1999; 7(3): 185–190.
[16] Supaluk P, Puttirat S, Rungrot C, Somsak R, Virapong P. New bioactive triterpenoids and antimalarial activity of Diospyros rubra Lec. EXCLI J. 2009; 9: 1-10.
[17] Appleton RA, Enzell CR. Triterpenoids and aromatic components of deertongue leaf. Phytochemistry 1971; 10: 447–449. [CrossRef]
[18] Moghaddam FM, Farimani MM, Salahvarzi S, Amin G. Chemical constituents of dichloromethane extract of cultivated Satureja khuzistanica. Evid Based Complement Alternat Med. 2007;4(1):95-98. [CrossRef]
[19] Yang AM, Li H, Liu JL, Guo WJ, Wu R. Chemical constituents of Euphorbia altotibetica. Adv Mater Res. 2013; 634-638: 905-908. [CrossRef]
[20] Xu Y, Wei L. Chemical constituents of Astragalus chinensis L. China J Chinese Mat Med. 1995; 20(5): 297-320.
[21] Wang YN, Cai JY, Zhao L, Zhu E, Zhang DZ. Study on chemical constituents from Anoectochilus chapaensis, China J Chinese Mat Med. 2012; 35: 911-913.
[22] Komal R, Zubair M, Rasool N, Riaz M, Zia-Ul-Haq M, de Feo V. Phytochemical and biological studies of Agave attenuate. Int J Mol Sci. 2012; 13: 6440-6451. [CrossRef]
[23] Strube M, Haenen GRMM, Van Den Berg H, Bast A. Pitfalls in a method for the measurement of total antioxidant capacity. Free Radi Res. 1997; 26: 512-521.
[24] Yi-Zhhong C, Mei S, Jie X, Qiong L, Harold C. Structure-radical scavenging activity relationship of phenolic compounds from traditional Chinese medicinal plants. Life Sci. 2006; 78: 2872-2888. [CrossRef]
[25] Benzie IFF, Strain JJ. The ferric reducing ability of plasma as a measure of antioxidant power: the FRAP assay. Anal Biochem. 1996; 239: 70-76.
[26] Kim YJ, Uyama H. Review, tyrosinase inhibitors from natural and synthetic sources: structures, inhibition mechanism and perspective for the future. Cell Mol Life Sci. 2005; 62: 1707-1723. [CrossRef]
[27] Magid AA, Laurence V, Bontemps G, Lituudon M, Lavaud C. Tyrosinase inhibitors and sesquiterpene diglycosides from Guioa villosa. Planta Med. 2008; 74: 55-60. [CrossRef]
[28] Mohsen M, Neda A, Eko S, Shajarahtunnur J, Fadzilah AA, Shahpour K. Total phenolic content, antioxidant and antibacterial properties of Scurrula ferruginea extracts. J Teknol. 2014; 70(5): 65-72.
[29] Hashim NM, Rahmani M, Ee GC, Sukari MA, Yahayu M, Amin MA, Ali AM, Go R. Antioxidant antimicrobial and tyrosinase inhibitiry activities of xanthones isolated from Artocarpus obtusus F.M. Jarret. Molecules 2012; 17: 6071-6082. [CrossRef]
[30] Zou Y, Chang SKC, Gu Y, Qian SY. Antioxidant activity and phenolic composition of lentils (Lens Culinaris var. Morton) extracts and its fractions. J Agric Food Chem. 2011; 59: 2268-2276. [CrossRef]
[31] Channarong A, Jutiviboonsuk A, Korsanan S. Total reducing antioxidant capacity of Thai herbal aromatic powder (Ya-Hom) measured by FRAP Assay. Thai Pharm Health Sci. J 2012; 7(3): 111-114.
[32] Shahwar D, Raza MA, Bukhari S, Bukhari G. Ferric reducing antioxidant power of essential oils extracted from Eucalyptus and Curcuma species. Asian Pac J Trop Biomed. 2012; S1633-S1636. [CrossRef]
[33] Seladji M, Bekhechi C, Beddou F, Dib H, Bendimerad N. Antioxidant activity and phytochemical screening of Nepeta nepetella aqueous and methanolic extracts from Algeria. J Appl Pharm Sci. 2014; 4(2): 012-016. [CrossRef]
[34] Likhitwitayawuid K, Sritularak. A new dimeric stilbene with tyrosinase inhibitory activity from Artocarpus gomezianus. J Nat Prod. 2001; 64: 1457-1459. [CrossRef]
[35] Promden W, Viriyabancha W, Monthakantirat O, Umehara K, Noguchi H, De-Eknamkul W. Correlation between the potency of flavonoids on mushroom tyrosinase inhibitory activity and melanin synthesis in melanocytes. Molecules 2018; 23: 1403. [CrossRef]
[36] Gulluce M, Sokmen M, Sahin F, Sokmen A, Adiguzel A, Ozer H. Biological activities of the essential oil and methanolic Micromeria frustica (L) Druce ssp seryllifolia (Bieb) PH Davis plants from the eastern Anatolia region of Turkey. J Sci Food Agric. 2004; 84: 735–741.
[37] Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. Manual of clinical microbiology. 7th Ed.; ASM: Washington DC, USA.

Details

Primary Language

English

Subjects

Pharmaceutical Chemistry

Journal Section

Research Article

Authors

Kamal Ja’afar Muhammad This is me
Malaysia

Shajarahtunnur Jamil ^* This is me
Malaysia

Norazah Basar This is me
Malaysia

Publication Date

June 27, 2025

Submission Date

September 18, 2018

Acceptance Date

December 7, 2018

Published in Issue

Year 2019 Volume: 23 Number: 3

IZ

https://izlik.org/JA43YT44MF

Cite

RIS / Bibtex

APA

Muhammad, K. J., Jamil, S., & Basar, N. (2025). Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves. Journal of Research in Pharmacy, 23(3), 522-531. https://izlik.org/JA43YT44MF

AMA

1.Muhammad KJ, Jamil S, Basar N. Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves. J. Res. Pharm. 2025;23(3):522-531. https://izlik.org/JA43YT44MF

Chicago

Muhammad, Kamal Ja’afar, Shajarahtunnur Jamil, and Norazah Basar. 2025. “Phytochemical Study and Biological Activities of Scurrula Parasitica L (Loranthaceae) Leaves”. Journal of Research in Pharmacy 23 (3): 522-31. https://izlik.org/JA43YT44MF.

EndNote

Muhammad KJ, Jamil S, Basar N (June 1, 2025) Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves. Journal of Research in Pharmacy 23 3 522–531.

IEEE

[1]K. J. Muhammad, S. Jamil, and N. Basar, “Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves”, J. Res. Pharm., vol. 23, no. 3, pp. 522–531, June 2025, [Online]. Available: https://izlik.org/JA43YT44MF

ISNAD

Muhammad, Kamal Ja’afar - Jamil, Shajarahtunnur - Basar, Norazah. “Phytochemical Study and Biological Activities of Scurrula Parasitica L (Loranthaceae) Leaves”. Journal of Research in Pharmacy 23/3 (June 1, 2025): 522-531. https://izlik.org/JA43YT44MF.

JAMA

1.Muhammad KJ, Jamil S, Basar N. Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves. J. Res. Pharm. 2025;23:522–531.

MLA

Muhammad, Kamal Ja’afar, et al. “Phytochemical Study and Biological Activities of Scurrula Parasitica L (Loranthaceae) Leaves”. Journal of Research in Pharmacy, vol. 23, no. 3, June 2025, pp. 522-31, https://izlik.org/JA43YT44MF.

Vancouver

1.Kamal Ja’afar Muhammad, Shajarahtunnur Jamil, Norazah Basar. Phytochemical study and biological activities of Scurrula parasitica L (Loranthaceae) leaves. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(3):522-31. Available from: https://izlik.org/JA43YT44MF