Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections

Fawzi Irshaid Irshaid; Samia Ramadan Eshawakh; Abdelrahim Hunaiti; Harbi Tomah Al-masri

Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections

Abstract

There is a focus on using aromatic phosphinous amide as an antibacterial agent against serious multidrug resistant bacterial infections. N–(4–acetylphenyl)–P,P–diphenyl-phosphinous amide and its corresponding thioxo- phosphinous amide and selenoxo-phosphinous amide were synthesized and designated Ph1, Ph2, and Ph3, respectively. They were structurally identified by single crystal X-ray diffraction, multinuclear NMR spectroscopy, and elemental analysis. Because their antibacterial activities have never been examined, this study aimed to assess their antibacterial activities against both Gram-positive and Gram-negative bacteria as well as readily available standard bacterial strains. Two bacterial isolates were isolated from urine specimens collected from patients with urinary tract infections and designated CS1, and CS2. Phenotypic, biochemical, and molecular approaches were used to identify them. The antibiotic susceptibility/resistance pattern of these isolates was examined by the installed VITEK 2 system. Isolate CS1 is an aerobic Gram-positive, straight rod-shaped, spore forming, occurring singly or in a short chain, whereas isolate CS2 is an aerobic Gram-negative, coccobacilli-shaped, non-spore forming, occurring in pairs or in chains. Both isolates are positive for catalase and oxidase test. According to an examination of the 16S rRNA gene sequence, the isolates CS1 and CS2 have 98% of their similarities with Bacillus and Acinetobacter species, respectively. Isolates CS1 and CS2 are multidrug resistance species. Based on the results of the minimum inhibitory concentration, the standard bacterial strains and isolates CS1 and CS2 showed a wide range in the antibacterial capabilities of the studied phosphinous amides. In comparison to Ph2 and Ph3, Ph1 has the strongest antibacterial activity against all of the tested bacterial species. In conclusion, CS1 and CS2 isolates were identified as novel, multi-drug resistant members of the Bacillus and Acinetobacter genera, respectively. The Ph1 molecule represents a promising new generation of antibiotics with notable antibacterial efficacy against multidrug-resistant Gram-positive and Gram-negative microorganisms.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Fawzi Irshaid Irshaid ^* This is me
0000-0003-0364-3135
Jordan

Samia Ramadan Eshawakh This is me
0009-0007-8642-4902
Jordan

Abdelrahim Hunaiti This is me
0000-0003-4737-6999
Jordan

Harbi Tomah Al-masri This is me
0000-0001-6928-1152
Jordan

Publication Date

June 28, 2025

Submission Date

April 18, 2023

Acceptance Date

September 10, 2023

Published in Issue

Year 2024 Volume: 28 Number: 5

IZ

https://izlik.org/JA46YK33YX

Cite

RIS / Bibtex

APA

Irshaid, F. I., Eshawakh, S. R., Hunaiti, A., & Al-masri, H. T. (2025). Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections. Journal of Research in Pharmacy, 28(5), 1800-1811. https://izlik.org/JA46YK33YX

AMA

1.Irshaid FI, Eshawakh SR, Hunaiti A, Al-masri HT. Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections. J. Res. Pharm. 2025;28(5):1800-1811. https://izlik.org/JA46YK33YX

Chicago

Irshaid, Fawzi Irshaid, Samia Ramadan Eshawakh, Abdelrahim Hunaiti, and Harbi Tomah Al-masri. 2025. “Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections”. Journal of Research in Pharmacy 28 (5): 1800-1811. https://izlik.org/JA46YK33YX.

EndNote

Irshaid FI, Eshawakh SR, Hunaiti A, Al-masri HT (July 1, 2025) Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections. Journal of Research in Pharmacy 28 5 1800–1811.

IEEE

[1]F. I. Irshaid, S. R. Eshawakh, A. Hunaiti, and H. T. Al-masri, “Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections”, J. Res. Pharm., vol. 28, no. 5, pp. 1800–1811, July 2025, [Online]. Available: https://izlik.org/JA46YK33YX

ISNAD

Irshaid, Fawzi Irshaid - Eshawakh, Samia Ramadan - Hunaiti, Abdelrahim - Al-masri, Harbi Tomah. “Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections”. Journal of Research in Pharmacy 28/5 (July 1, 2025): 1800-1811. https://izlik.org/JA46YK33YX.

JAMA

1.Irshaid FI, Eshawakh SR, Hunaiti A, Al-masri HT. Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections. J. Res. Pharm. 2025;28:1800–1811.

MLA

Irshaid, Fawzi Irshaid, et al. “Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections”. Journal of Research in Pharmacy, vol. 28, no. 5, July 2025, pp. 1800-11, https://izlik.org/JA46YK33YX.

Vancouver

1.Fawzi Irshaid Irshaid, Samia Ramadan Eshawakh, Abdelrahim Hunaiti, Harbi Tomah Al-masri. Aromatic Phosphinous Amides: A Promising New Generation of Antibiotics for Multidrug-Resistant Bacterial Infections. J. Res. Pharm. [Internet]. 2025 Jul. 1;28(5):1800-11. Available from: https://izlik.org/JA46YK33YX