HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract

Mohamed Malik Mahdjoub; Nourelimane Benzitoune; Yacine Maiz; Nour El Houda Aouadi; Mustapha Mounir Bouhenna; Nabil Kadri

HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract

Abstract

Salvia balansae de Noé a have known to be an important source of a huge variety of no toxic bioactive compounds with lack of information hinders exploitation in food and pharmaceutical industries as natural antioxidants. In this work, the phenolic compounds from S. balansae leaves were extracted using an ultrasound-assisted methodology and were separated and identified by HPLC-DAD. The antioxidant activity was evaluated by ABTS, and DPPH scavenging assays, Iron (Fe2+) Chelating ability and reducing power (FRAP) tests. The total phenolic compounds (TPC) and total flavonoids content (TFC) were found with amounts of 49.63 ± 6.31 mg GAE/g DW and 8.14 ± 0.39 mg QE/g DW respectively. The ABTS, DPPH assays and metal chelating ability test demonstrated a higher antioxidant properties with IC50 values of 328.95 ± 5.29 µg/mL, 545.03 ± 3.267 µg/mL and 689.40 ± 0.86 µg/mL respectively. The reducing power at 700 nm increased considerably with concentration. In addition, the HPLC-DAD analysis showed that there were twelve detectable phenolic compounds and the benzoic acid, quercetin, myrecetin, hydroxyflavon, and ascorbic acid have been exclusively identified for the first time in S. balansae leaves extract and are rarely determined in other Salvia L. species. Therefore, these results brought new data about the main phenolic compounds presents in S. balansae leaves extract with a strong antioxidant potential, which will be an alternative source for synthetic compounds.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Mohamed Malik Mahdjoub This is me
0000-0001-5584-6642
Algeria

Nourelimane Benzitoune This is me
0009-0004-4563-4362
Algeria

Yacine Maiz This is me
Algeria

Nour El Houda Aouadi This is me
Algeria

Mustapha Mounir Bouhenna This is me
0000-0001-6789-5636
Algeria

Nabil Kadri
0000-0003-1158-696X
Algeria

Publication Date

June 28, 2025

Submission Date

October 1, 2022

Acceptance Date

January 29, 2023

Published in Issue

Year 2023 Volume: 27 Number: 3

IZ

https://izlik.org/JA73US22HX

Cite

RIS / Bibtex

APA

Mahdjoub, M. M., Benzitoune, N., Maiz, Y., Aouadi, N. E. H., Bouhenna, M. M., & Kadri, N. (2025). HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract. Journal of Research in Pharmacy, 27(3), 1076-1085. https://izlik.org/JA73US22HX

AMA

1.Mahdjoub MM, Benzitoune N, Maiz Y, Aouadi NEH, Bouhenna MM, Kadri N. HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract. J. Res. Pharm. 2025;27(3):1076-1085. https://izlik.org/JA73US22HX

Chicago

Mahdjoub, Mohamed Malik, Nourelimane Benzitoune, Yacine Maiz, Nour El Houda Aouadi, Mustapha Mounir Bouhenna, and Nabil Kadri. 2025. “HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia Balansae de Noé Leaves Extract”. Journal of Research in Pharmacy 27 (3): 1076-85. https://izlik.org/JA73US22HX.

EndNote

Mahdjoub MM, Benzitoune N, Maiz Y, Aouadi NEH, Bouhenna MM, Kadri N (June 1, 2025) HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract. Journal of Research in Pharmacy 27 3 1076–1085.

IEEE

[1]M. M. Mahdjoub, N. Benzitoune, Y. Maiz, N. E. H. Aouadi, M. M. Bouhenna, and N. Kadri, “HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract”, J. Res. Pharm., vol. 27, no. 3, pp. 1076–1085, June 2025, [Online]. Available: https://izlik.org/JA73US22HX

ISNAD

Mahdjoub, Mohamed Malik - Benzitoune, Nourelimane - Maiz, Yacine - Aouadi, Nour El Houda - Bouhenna, Mustapha Mounir - Kadri, Nabil. “HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia Balansae de Noé Leaves Extract”. Journal of Research in Pharmacy 27/3 (June 1, 2025): 1076-1085. https://izlik.org/JA73US22HX.

JAMA

1.Mahdjoub MM, Benzitoune N, Maiz Y, Aouadi NEH, Bouhenna MM, Kadri N. HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract. J. Res. Pharm. 2025;27:1076–1085.

MLA

Mahdjoub, Mohamed Malik, et al. “HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia Balansae de Noé Leaves Extract”. Journal of Research in Pharmacy, vol. 27, no. 3, June 2025, pp. 1076-85, https://izlik.org/JA73US22HX.

Vancouver

1.Mohamed Malik Mahdjoub, Nourelimane Benzitoune, Yacine Maiz, Nour El Houda Aouadi, Mustapha Mounir Bouhenna, Nabil Kadri. HPLC-DAD Screening and Antioxidant Activity of Phenolic Compounds of Salvia balansae de Noé Leaves Extract. J. Res. Pharm. [Internet]. 2025 Jun. 1;27(3):1076-85. Available from: https://izlik.org/JA73US22HX